Expression of connexin 30 and connexin 32 in hippocampus of rat during epileptogenesis in a kindling model of epilepsy

Neuroscience Bulletin

Volumn 28, Issue 6, 2012, Pages 729-736

  Akbarpour, Bijan ^a,b   Sayyah, Mohammad ^a   Babapour, Vahab ^c   Mahdian, Reza ^a   Beheshti, Siamak ^d   Kamyab, Ahmad Reza ^a  

^a PASTEUR INSTITUTE OF IRAN   (Iran)

^b ISLAMIC AZAD UNIVERSITY   (Iran)

^c UNIVERSITY OF TEHRAN   (Iran)

^d UNIVERSITY OF ISFAHAN   (Iran)

Author keywords

Connexin 30; Connexin 32; Hippocampus; Kindling

Indexed keywords

CONNEXIN 30; CONNEXIN 32; MESSENGER RNA; COMPLEMENTARY DNA; CONNEXIN 30, RAT; GAP JUNCTION PROTEIN; RNA;

AFTERDISCHARGE; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BASOLATERAL AMYGDALA; CONTROLLED STUDY; DOWN REGULATION; EPILEPSY; EPILEPTOGENESIS; FOCAL EPILEPSY; GENE EXPRESSION ASSAY; HIPPOCAMPUS; KINDLING; MALE; NONHUMAN; PROTEIN EXPRESSION; RAT; REAL TIME POLYMERASE CHAIN REACTION; STEREOTAXIC SURGERY; STEREOTAXIS; TONIC CLONIC SEIZURE; UPREGULATION; WESTERN BLOTTING; ANIMAL; BIOSYNTHESIS; ELECTROSTIMULATION; GAP JUNCTION; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PHYSIOLOGY; SEIZURE; STEREOTACTIC PROCEDURE; WISTAR RAT;

ANIMALS; BLOTTING, WESTERN; CONNEXINS; DNA, COMPLEMENTARY; ELECTRIC STIMULATION; EPILEPSY; GAP JUNCTIONS; GENE EXPRESSION REGULATION; HIPPOCAMPUS; KINDLING, NEUROLOGIC; MALE; RATS; RATS, WISTAR; REAL-TIME POLYMERASE CHAIN REACTION; RNA; SEIZURES; STEREOTAXIC TECHNIQUES;

EID: 84904524462     PISSN: 16737067     EISSN: 19958218     Source Type: Journal    
DOI: 10.1007/s12264-012-1279-6     Document Type: Article

References (32)

1. Vezzani M, French J, Bartfai T, Baram TZ. The role of inflammation in epilepsy. Nat Rev Neurol 2011, 7: 31-40.
2. Perez-Velazquez JL, Carlen PL. Gap junctions, synchrony and seizure. Trends Neurosci 2000, 23: 68-74.
3. Jin MM, Chen Z. Role of gap junctions in epilepsy. Neurosci Bull 2011, 27: 389-406.
4. Li J, Shen H, Naus CC, Zhang L, Carlen PL. Upregulation of gap junction connexin 32 with epileptiform activity in the isolated mouse hippocampus. Neuroscience 2001, 105: 589-598.
5. Rouach N, Avignone E, Meme W, Koulakoff A, Venance L, Blomstrand F, et al. Gap junctions and connexin expression in the normal and pathological central nervous system. Biol Cell 2002, 94: 451-475.
6. Beheshti S, Sayyah M, Golkar M, Sepehri H, Babaie J, Vaziri B. Changes in hippocampal connexin 36 mRNA and protein levels during epileptogenesis in the kindling model of epilepsy. Prog Neuropsychopharmacol Biol Psychiatry 2010, 34: 510-515.
7. Elisevich K, Rempel SA, Smith B, Allar N. Connexin 43 mRNA expression in two experimental models of epilepsy. Mol Chem Neuropathol 1997, 32: 75-88.
8. Söhl G, Maxeiner S, Willecke K. Expression and functions of neuronal gap junctions. Nat Rev Neurosci 2005, 6: 191-200.
9. McCracken CB, Roberts DCS. A single evoked afterdischarge produces rapid time-dependent changes in connexin 36 protein expression in adult rat dorsal hippocampus. Neurosci Lett 2006, 405: 84-88.
10. Pannasch U, Vargová L, Reingrube J, Ezan P, Holcman D, Giaume C, et al. Astroglial networks scale synaptic activity and plasticity. Proc Natl Acad Sci U S A 2011, 108: 8467-8472.
11. Garbelli R, Frassoni C, Condorelli DF, Trovato Salinaro A, Musso N, Medici V, et al. Expression of connexin 43 in the human epileptic and drug-resistant cerebral cortex. Neurology 2011, 76: 895-902.
12. Condorelli DF, Mudò G, Trovato-Salinaro A, Mirone MB, Amato G, Belluardo N. Connexin-30 mRNA is up-regulated in astrocytes and expressed in apoptotic neuronal cells of rat brain following kainateinduced seizures. Mol Cell Neurosci 2002, 21: 94-113.
13. Condorelli DF, Trovato-Salinaro A, Mudo G, Mirone MB, Belluardo N. Cellular expression of connexins in the rat brain: neuronal localization, effects of kainate-induced seizures and expression in apoptotic neuronal cells. Eur J Neurosci 2003; 18: 1807-1827.
14. Szente M, Gajda Z, Said Ali K, Hermesz E. Involvement of electrical coupling in the in vivo ictal epileptiform activity induced by 4-Aminopyridine in the neocortex. Neuroscience 2002, 115: 1067-1078.
15. Collignon F, Wetjen NM, Cohen-Gadol AA, Cascino GD, Parisi J, Meyer FB, et al. Altered expression of connexin subtypes in mesial temporal lobe epilepsy in humans. J Neurosurg 2006, 105: 77-87.
16. Magnotti LM, Goodenough DA, Paul DL. Deletion of oligodendrocyte Cx32 and astrocyte Cx43 causes white matter vacuolation, astrocyte loss and early mortality. Glia 2011, 59: 1064-1074.
17. Yao LF, Wang ZK, Wang ZG, Sui D, Zhang LM. Expression and function of Cx32 and Cx43 junctions in medically intractable temporal lobe epilepsy in human. Natl Med J Chin 2009, 89: 3058-3060.
18. Gajda Z, Gyengesi E, Hermesz E, Ali KS, Szente M. Involvement of gap junctions in the manifestation and control of the duration of seizures in rats in vivo. Epilepsia 2003, 44: 1596-1600.
19. Zhang Y, Shan W, Zhang G. The relations between electrical synapse and the mechanism of epilepsy in genetically epilepsy-prone rat. Ntal Med J Chin 1998, 78: 311-313.
20. Racine RJ. Modification of seizure activity by electrical stimulation. II. Motor seizure. Electroencephalogr Clin Neurophysiol 1972, 32: 281-294.
21. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, et al. Short Protocols in Molecular Biology. 5th ed. New York: Wiley, 2002: 235.
22. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C (T)) Method. Methods 2001, 25: 402-408.
23. Vaerman JL, Saussoy P, Ingargiola I. Evaluation of real-time PCR data. J Biol Regul Homeost Agents 2004, 18: 212-214.
24. Kielian T. Glial connexins and gap junctions in CNS inflammation and disease. J Neurochem 2008, 106: 1000-1016.
25. Temme A, Traub O, Willecke K. Downregulation of connexin32 protein and gap-junctional intercellular communication by cytokine-mediated acute-phase response in immortalized mouse hepatocyte. Cell Tissue Res 1998, 294: 345-350.
26. Rouach N, Calvo CF, Glowinski J, Giaume C. Brain macrophages inhibit gap junctional communication and downregulate connexin 43 expression in cultured astrocytes. Eur J Neurosci 2002, 15: 403-447.
27. Nagy JI, Patel D, Ochalski PAY, Stelmack GL. Connexin 30 in rodent, cat and human brain: selective expression in gray matter astrocytes, co-localization with connexin43 at gap junctions and late developmental appearance. Neuroscience 1999, 88: 447-468.
28. Rouach N, Koulakoff A, Abudara V, Willecke K, Giuame C. Astroglial metabolic networks sustain synaptic transmission. Science 2008, 322: 1551-1555.
29. Gosejacob D, Dublin P, Bedner P. Role of astroglial connexin30 in hippocampal gap junction coupling. Glia 2011, 59: 511-519.
30. Wallraff A, Köhling R, Hinemann U, Theis M, Willecke K, Steinhauser C. The impact of astrocytic gap junctional coupling on potassium buffering in the hippocampus. J Neurosci 2006, 26: 5438-5447.
31. Bennet SAL, Arnold JM, Chen J, Stenger J, Paul DL, Roberts DCS. Long-term changes in connexin32 gap junction protein and mRNA expression following cocaine self-administration in rats. Eur J Neurosci 1999, 11: 3329-3338.
32. Oguro K, Jover T, Tanaka H, Lin Y, Kojima T, Oguro N, Grooms SY, Bennet MVL, Zukin RS. Global ischemia-induced increases in the gap junctional proteins connexin32 (Cx32) and Cx36 in hippocampus and enhanced vulnerability of Cx32 knock-out mice. J Neurosci 2001, 21: 7534-7542.