Evaluation of real-time PCR data

Journal of Biological Regulators and Homeostatic Agents

Volumn 18, Issue 2, 2004, Pages 212-214

  Vaerman, J L ^a   Saussoy, P ^a   Ingargiola, I ^a  

^a CLINIQUES UNIVERSITAIRES SAINT LUC   (Belgium)

Author keywords

Amplication; PCR; Quantification; Real time PCR; Validation

Indexed keywords

ACCURACY; ARTICLE; DATA ANALYSIS; MOLECULAR BIOLOGY; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; SENSITIVITY AND SPECIFICITY;

EID: 5744232826     PISSN: 0393974X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (9)

1. Higuchi R, Dollinger G, Walsh PS, et al. Simultaneous amplification and detection of specific DNA sequences. Biotechnology 1992; 10: 413-7.
2. Bustin SA. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 2000; 25: 169-93.
3. Ct method. Methods 2001; 25: 402-8.
4. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 2001; 29: 2002-7.
5. Vandesompele J, De Preter K, Pattyn F, et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 2002; 3: 0034.
6. Ramakers C, Ruijter JM, Deprez RH, Moorman AF. Assumption-free analysis of quantitative real-time PCR data. Neurosci Lett 2003; 339: 62-6.
7. Rasmussen R, Morrison T, Herrman M, et al. Quantitative PCR by continuous fluorescence monitoring of a double strand DNA-specific bibding dye. Biochemica 1998; 2: 8-11.
8. Holland PM, Abramson RD, Watson R, et al. Detection of specific polymerase chain reaction product by utizing the 5′ to 3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc Natl Acad Sci USA 1991; 88: 7276-80.
9. Saussoy P, Vaerman JL, Straetmans N, et al. Differentiation of acute myeloid leukemia from B- and T-lineage acute lymphoid leukemias by real-time quantitative reverse transcription-PCR of lineage marker mRNA. Clin Chem 2004; 50: 1165-73.