Effects of nutrient levels and average culture pH on the glycosylation pattern of camelid-humanized monoclonal antibody

Journal of Biotechnology

Volumn 186, Issue , 2014, Pages 98-109

  Aghamohseni, Hengameh ^a   Ohadi, Kaveh ^a   Spearman, Maureen ^b   Krahn, Natalie ^b   Moo Young, Murray ^a   Scharer, Jeno M ^a   Butler, Mike ^b   Budman, Hector M ^a  

^a UNIVERSITY OF WATERLOO   (Canada)

^b UNIVERSITY OF MANITOBA   (Canada)

Author keywords

Dynamic modeling; Glycosylation; Glycosylation index; Mammalian cell culture; Monoclonal antibody

Indexed keywords

AMINO ACIDS; CELL CULTURE; CELL GROWTH; DYNAMIC MODELS; ESTERIFICATION; GROWTH KINETICS; METABOLISM; MONOCLONAL ANTIBODIES; NUTRIENTS; PH EFFECTS; PRODUCTIVITY; GLYCOSYLATION;

CHINESE HAMSTER OVARY CELLS; GLUCOSE CONSUMPTION; HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHIES; INITIAL CONCENTRATION; MAMMALIAN CELL CULTURE; METABOLIC FLUX ANALYSIS; MONOCLONAL ANTIBODIES (MAB); OPERATING CONDITION; HUMANIZED MONOCLONAL ANTIBODY; NUTRIENT LEVELS;

GLYCOSYLATION; DYNAMIC MODELS;

AMMONIA; GLUCOSE; GLUTAMINE; GLYCAN; MONOCLONAL ANTIBODY; GLUTAMIC ACID; LACTIC ACID;

AMMONIA FORMATION; ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; ARTIODACTYLA; BIOMASS; CELL DENSITY; CELL GROWTH; CELL METABOLISM; CELL VIABILITY; CHEMICAL COMPOSITION; CHO CELL LINE; CITRIC ACID CYCLE; DEPLETION; FEMALE; GLUCOSE INTAKE; GLYCOLYSIS; GLYCOSYLATION; HYDROPHILIC INTERACTION CHROMATOGRAPHY; METABOLIC FLUX ANALYSIS; NONHUMAN; NUTRIENT; PH; PRIORITY JOURNAL; PRODUCTIVITY; SIALYLATION; ANIMAL; BIOLOGICAL MODEL; CHEMISTRY; CRICETULUS; CULTURE TECHNIQUE; EXTRACELLULAR SPACE; HAMSTER; METABOLISM; PROCEDURES; CELL COUNTING;

ANIMALS; ANTIBODIES, MONOCLONAL, HUMANIZED; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETINAE; CRICETULUS; EXTRACELLULAR SPACE; GLUCOSE; GLUTAMINE; GLYCOSYLATION; HYDROGEN-ION CONCENTRATION; MODELS, BIOLOGICAL;

EID: 84904490835     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2014.05.024     Document Type: Article

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