Sequencing of N-linked oligosaccharides directly from protein gels: In- gel deglycosylation followed by matrix-assisted laser desorption/ionization mass spectrometry and normal-phase high-performance liquid chromatography

Analytical Biochemistry

Volumn 250, Issue 1, 1997, Pages 82-101

  Küster, Bernhard ^a   Wheeler, Susan F ^a   Hunter, Ann P ^a   Dwek, Raymond A ^a   Harvey, David J ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DESORPTION; ELECTROPHORESIS; ESTERIFICATION; GELS; GLYCOPROTEINS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MAMMALS; MASS SPECTROMETRY; POLYSACCHARIDES; PROTEINS; PURIFICATION; SODIUM DODECYL SULFATE; VIRUSES;

ALPHA-1-ACID GLYCOPROTEIN; CHINESE HAMSTER OVARY CELLS; FLUORESCENT LABELING; HEAVY AND LIGHT CHAINS; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNOGLOBULIN G; MATRIX-ASSISTED LASER DESORPTION/IONIZATION MASS SPECTROMETRY; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS;

OLIGOSACCHARIDES;

ASPARAGINE LINKED OLIGOSACCHARIDE; ENZYME INHIBITOR; FETUIN; GLYCAN; GLYCOPROTEIN; GLYCOPROTEIN GP 120; IMMUNOGLOBULIN G; MIGLUSTAT; OROSOMUCOID; OVALBUMIN; RIBONUCLEASE;

ANALYTIC METHOD; ARTICLE; DESORPTION; ENZYME INHIBITION; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; IONIZATION; LASER; MASS SPECTROMETRY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN STRUCTURE; STRUCTURE ACTIVITY RELATION;

BOVINAE; CRICETINAE; CRICETULUS GRISEUS; GALLUS GALLUS; HUMAN IMMUNODEFICIENCY VIRUS;

EID: 0031571138     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1997.2199     Document Type: Article

References (41)

1. Dwek R. A. Chem. Rev. 96:1996;683-720.
2. Dwek R. A. Biochem. Soc. Trans. 23:1995;1-25.
3. Varki A. Glycobiology. 3:1993;97-130.
4. Takasaki S., Mizuochi T., Kobata A. Methods Enzymol. 83:1982;263-268.
5. Patel P., Bruce J., Merry A., Bigge C., Wormald M., Jaques A., Parekh R. Biochemistry. 32:1993;679-693.
6. Tarentino A. L., Gomez C. M., Plummer T. H. Jr. Biochemistry. 24:1985;4665-4671.
7. VanHalbeek H. Methods Enzymol. 230:1994;132-168.
8. Harvey D. J., Rudd P. M., Bateman R. H., Bordoli R. S., Howes K., Hoyes J. B., Vickers R. G. Org. Mass Spectrom. 29:1994;753-765.
9. Guile G. R., Rudd P. M., Wing D. R., Prime S. B., Dwek R. A. Anal. Biochem. 240:1996;210-226.
10. Tretter V., Altmann F., März L. Eur. J. Biochem. 199:1991;647-652.
11. Wilm M., Shevchenko A., Houthaeve T., Breit S., Schweigerer L., Fotsis T., Mann M. Nature. 379:1996;466-469.
12. Weitzhandler M., Kadlacek D., Avdalovic N., Forte J. G., Chow D., Townsend R. R. J. Biol. Chem. 268:1993;5121-5130.
13. Andersen D. C., Goochee C. F., Cooper G., Weitzhandler M. Glycobiology. 4:1994;459-467.
14. Mortz E., Sareneva T., Haebel S., Julkunen I., Roepstorff P. Electrophoresis. 17:1996;925-931.
15. Wilm M., Neubauer G., Mann M. Anal. Chem. 68:1996;527-533.
16. Köhler G. Hybridoma Techniques. 1980;Cold Spring Harbor Laboratories, Cold Spring Harbor.
17. Lee C., Levin A., Branton D. Anal. Biochem. 166:1987;308-312.
18. R. S. Annan, D. McNulty, S. A. Carr, May 1996, Proc, 44th ASMS Conference on Mass Spectrometry and Allied Topics, 12, 16, Portland, Oregon.
19. Powell A. K., Harvey D. J. Rapid Commun. Mass Spectrom. 10:1996;1027-1032.
20. P. Roepstorff, H. R. Nielsen, M. R. Larsen, S. Haebel, C. Jensen, L. Palm, T. Krogh, E. Mirgorodskaya, E. Nordhoff, M. Kussmann, May 1996, Proc, 44th ASMS Conference on Mass Spectrometry and Allied Topics, 12, 16, Portland, Oregon.
21. Bigge J. C., Patel T. P., Bruce A., Goulding P. N., Charles S. W. M., Parekh R. B. Anal. Biochem. 230:1995;229-238.
22. Mock K. K., Davey M., Cottrell J. S. Biochem. Biophys. Res. Commun. 177:1991;644-651.
23. Mock K. K., Sutton C. W., Cottrell J. S. Rapid Commun. Mass Spectrom. 6:1992;233-238.
24. Küster B., Harvey D. J. Glycobiology. 7:1997;vii-ix.
25. Naven T. J. P., Harvey D. J. Rapid Commun. Mass Spectrom. 10:1996;1361-1366.
26. Harvey D. J. Rapid Commun. Mass Spectrom. 7:1993;614-619.
27. Vestal M. L., Juhasz P., Martin S. A. Rapid Commun. Mass Spectrom. 9:1995;1044-1050.
28. Rudd P. M., Scragg I. G., Coghill E., Dwek R. A. Glycoconjugate J. 9:1992;86-91.
29. Küster B., Naven T. J. P., Harvey D. J. J. Mass Spectrom. 31:1996;1131-1140.
30. Green E. D., Adelt G., Baenziger J. E., Wilson S., vanHalbeek H. J. Biol. Chem. 263:1988;18253-18268.
31. Nashabeh W., Rassi Z. J. Chromatogr. 536:1991;31-42.
32. Hunter A. P., Games D. E. Rapid Commun. Mass Spectrom. 9:1995;42-56.
33. Shevchenko A., Wilm M., Vorm O., Mann M. Anal. Chem. 68:1996;850-858.
34. Mizuochi T., Spellman M. W., Larkin M., Solomon J., Basa L. J., Feizi T. Biochem. J. 254:1988;599-903.
35. Mizuochi T., Spellman M. W., Larkin M., Solomon J., Basa L. J., Feizi T. Biomed. Chromatogr. 2:1988;260-270.
36. Stanley P., Shantha T., Bhaumik M. Glycobiology. 6:1996;695-699.
37. Fleet G. W. J., Karpas A., Dwek R. A., Fellows L. E., Tyms A. S., Petursson S., Namgoon S. K., Ramsden N. G., Smith P. W., Son J. C., Wilson F., Witty D. R., Jacob G. S., Rademacher T. W. FEBS Lett. 237:1988;128-132.
38. Karpas A., Fleet G. W. J., Dwek R. A., Petursson S., Namgoon S. K., Ramsden N. G., Jacobs G. S., Rademacher T. W. Proc. Natl. Acad. Sci. USA. 85:1988;9229-9233.
39. Fischer P. B., Collin M., Karlsson G. B., James W., Butters T. D., Davis S. J., Gordon S., Dwek R. A., Platt F. M. J. Virol. 69:1995;5791-5797.
40. Karlsson G. B., Butters T. D., Dwek R. A., Platt F. M. J. Biol. Chem. 268:1993;570-576.
41. Lubas W. A., Spiro R. G. J. Biol. Chem. 263:1988;3990-3998.