Phenylboronic acid as a multi-modal ligand for the capture of monoclonal antibodies: Development and optimization of a washing step

Journal of Chromatography A

Volumn 1355, Issue , 2014, Pages 115-124

  dos Santos, Raquel ^a   Rosa, Sara A S L ^a   Aires Barros, M Raquel ^a   Tover, Andres ^b   Azevedo, Ana M ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

^b Icosagen Cell Factory ^*  (Estonia)

Author keywords

Capture step; MAbs purification; Multimodal chromatography; Phenylboronate ligands; Wash and elution studies

Indexed keywords

ALCOHOLS; BODY FLUIDS; CELL CULTURE; CHROMATOGRAPHY; LIGANDS; MONOCLONAL ANTIBODIES; ORGANIC ACIDS; PROTEINS; WASHING;

CAPTURE STEP; CHINESE HAMSTER OVARY CELLS; CHO CELL CULTURES; ISO-ELECTRIC POINTS; MULTIMODAL CHROMATOGRAPHIES; PHENYLBORONIC ACIDS; PROTEIN A CHROMATOGRAPHY; PURIFICATION FACTORS;

PURIFICATION;

BENZENEBORONIC ACID; IMMUNOGLOBULIN G; MATRIX PROTEIN; MONOCLONAL ANTIBODY; PROTEIN A; BORONIC ACID DERIVATIVE; LIGAND; STAPHYLOCOCCUS PROTEIN A;

ANIMAL CELL; ANTIBODY COMBINING SITE; ARTICLE; CELL CULTURE; CHROMATOGRAPHY; CONTROLLED STUDY; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN A CHROMATOGRAPHY; PROTEIN ANALYSIS; PROTEIN INTERACTION; PROTEIN PURIFICATION; WASHING STEP; AFFINITY CHROMATOGRAPHY; ANIMAL; CHEMISTRY; CHO CELL LINE; CRICETULUS; DEVICES; HAMSTER; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES;

CRICETULUS GRISEUS;

ANIMALS; ANTIBODIES, MONOCLONAL; BORONIC ACIDS; CHO CELLS; CHROMATOGRAPHY, AFFINITY; CRICETINAE; CRICETULUS; HUMANS; IMMUNOGLOBULIN G; LIGANDS; STAPHYLOCOCCAL PROTEIN A;

EID: 84904311566     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2014.06.001     Document Type: Article

References (33)

1. Chon J.H., Zarbis-Papastoitsis G. Advances in the production and downstream processing of antibodies. New Biotechnol. 2011, 28:458-463. 10.1016/j.nbt.2011.03.015.
2. Jain E., Kumar A. Upstream processes in antibody production: evaluation of critical parameters. Biotechnol. Adv. 2008, 26:46-72. 10.1016/j.biotechadv.2007.09.004.
3. Chu L., Robinson D.K. Industrial choices for protein production by large-scale cell culture. Curr. Opin. Biotechnol. 2001, 12:180-187. 10.1016/S0958-1669(00)00197-X.
4. Curling J. The development of antibody purifi{ligature}cation technologies. Process Scale Purifi{ligature}cation of Antibodies 2009, 25-52. Wiley, New Jersey. U. Gottschalk (Ed.).
5. Kelley B., Blank G., Lee A. Downstream processing of monoclonal antibodies: current practices and future opportunities. Process Scale Purification of Antibodies 2009, 1-23. Wiley, New York. U. Gottschalk (Ed.).
6. Liu H.F., Ma J., Winter C., Bayer R. Recovery and purification process development for monoclonal antibody production. mAbs 2010, 2:480-499. 10.4161/mabs.2.5.12645.
7. Shukla A.A., Thömmes J. Recent advances in large-scale production of monoclonal antibodies and related proteins. Trends Biotechnol. 2010, 28:253-261. 10.1016/j.tibtech.2010.02.001.
8. Azevedo A.M., Rosa P.A.J., Ferreira I.F., Aires-Barros M.R. Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing. Trends Biotechnol. 2009, 27:240-247. 10.1016/j.tibtech.2009.01.004.
9. Shukla A.A., Hubbard B., Tressel T., Guhan S., Low D. Downstream processing of monoclonal antibodies--application of platform approaches. J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 2007, 848:28-39. 10.1016/j.jchromb.2006.09.026.
10. Pakiman N., Abdulla N., Hassan M.A.A., Abdullah N., Walter J.K. Comparison of binding capacity and affinity of monoclonal antibody towards different affinity resins using high-throughput chromatography method. J. Appl. Sci. 2012, 12:1136-1141.
11. Liu Y., Lu Y., Liu Z. Restricted access boronate affinity porous monolith as a protein A mimetic for the specific capture of immunoglobulin G. Chem. Sci. 2012, 3:1467-1471. 10.1039/c2sc20125a.
12. Roque A.C.A., Lowe C.R., Taipa M.A. Antibodies and genetically engineered related molecules: production and purification. Biotechnol. Prog. 2004, 20:639-654. 10.1021/bp030070k.
13. Azevedo A.M., Gomes A.G., Borlido L., Santos I.F.S., Prazeres D.M.F., Aires-Barros M.R. Capture of human monoclonal antibodies from a clarified cell culture supernatant by phenyl boronate chromatography. J. Mol. Recognit. 2010, 23:569-576. 10.1002/jmr.1068.
14. Ren L., Liu Z., Dong M., Ye M., Zou H. Synthesis and characterization of a new boronate affinity monolithic capillary for specific capture of cis-diol-containing compounds. J. Chromatogr. A 2009, 1216:4768-4774. 10.1016/j.chroma.2009.04.036.
15. Borlido L., Azevedo A.M., Roque A.C.A., Aires-Barros M.R. Potential of boronic acid functionalized magnetic particles in the adsorption of human antibodies under mammalian cell culture conditions. J. Chromatogr. A 2011, 1218:7821-7827. 10.1016/j.chroma.2011.08.084.
16. Gomes A.G., Azevedo A.M., Aires-Barros M.R., Prazeres D.M.F. Studies on the adsorption of cell impurities from plasmid-containing lysates to phenyl boronic acid chromatographic beads. J. Chromatogr. A 2011, 1218:8629-8637. 10.1016/j.chroma.2011.10.004.
17. X.C. Liu, W.H. Scouten, Boronate affinity chromatography, in: P. Bailon, G.K. Ehrlich, W.J. Fung, W. Berthold (Eds.), Methods in Molecular Biology, Vol. 147: Affinity Chromatography: Methods and Protocols, Humana Press Inc., Totowa, NJ, 2000, pp. 119-128, doi:10.1385/1-59259-041-1:119.
18. Zhang B., Mathewson S., Chen H. Two-dimensional liquid chromatographic methods to examine phenylboronate interactions with recombinant antibodies. J. Chromatogr. A 2009, 1216:5676-5686. 10.1016/j.chroma.2009.05.084.
19. Bosch L.I., Fyles T.M., James T.D. Binary and ternary phenylboronic acid complexes with saccharides and Lewis bases. Tetrahedron 2004, 60:11175-11190. 10.1016/j.tet.2004.08.046.
20. D.G. Hall, Boronic Acids, in: D.G. Hall (Ed.), Boronic Acids: Preparation and Applications in Organic Synthesis, Medicine and Materials, second ed., Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany, 2011, pp. 1-9.
21. Brena B.M., Batista-Viera F., Rydén L., Porath J. Selective adsorption of immunoglobulins and glucosylated proteins on phenylboronate-agarose. J. Chromatogr 1992, 604:109-115. 10.1016/0021-9673(92)85535-2.
22. Silla T., Hääl I., Geimanen J., Janikson K., Abroi A., Ustav E., et al. Episomal maintenance of plasmids with hybrid origins in mouse cells. J. Virol. 2005, 79:15277-15288. 10.1128/JVI.79.24. 15277-15288.2005.
23. Azevedo A.M., Rosa P.A.J., Ferreira I.F., Aires-Barros M.R. Integrated process for the purification of antibodies combining aqueous two-phase extraction, hydrophobic interaction chromatography and size-exclusion chromatography. J. Chromatogr. A 2008, 1213:154-161. 10.1016/j.chroma.2008.09.115.
24. Total Protein Assays: Protein-to-protein variations, in: Pierce Protein Assay: Technical Handbook, 2012, p. 10.
25. Merk Millipore, ProSep® Ultra Plus Chromatography Media, 2012.
26. Springsteen G., Wang B. A detailed examination of boronic acid-diol complexation. Tetrahedron 2002, 58:5291-5300. 10.1016/S0040-4020(02)00489-1.
27. Li Y., Jeppsson J.-O., Jörntén-Karlsson M., Linné Larsson E., Jungvid H., Galaev I.Y., et al. Application of shielding boronate affinity chromatography in the study of the glycation pattern of haemoglobin. J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 2002, 776:149-160. 10.1016/S1570-0232(02)00162-9.
28. Quan C., Alcala E., Petkovska I., Matthews D., Canova-Davis E., Taticek R., et al. A study in glycation of a therapeutic recombinant humanized monoclonal antibody: where it is, how it got there, and how it affects charge-based behavior. Anal. Biochem. 2008, 373:179-191. 10.1016/j.ab.2007.09.027.
29. Ugwu S.O., Apte S.P. The effect of buffers on protein conformational stability. Pharm. Technol. 2004, 28:86-113.
30. Maury M., Murphy K., Kumar S., Mauerer A., Lee G. Spray-drying of proteins: effects of sorbitol and trehalose on aggregation and FT-IR amide I spectrum of an immunoglobulin G. Eur. J. Pharm. Biopharm. 2005, 59:251-261.
31. Rauch P., Dankbar N., Specht C., Sperling D. Optimisation of assys: Interference of immunoassays recognize and avoid. LABORWELT 2005, 6:2-7.
32. Cromwell M.E.M., Hilario E., Jacobson F. Protein aggregation and bioprocessing. Am. Assoc. Pharm. Sci. J. 2006, 8:572-579. 10.1208/aapsj080366.
33. Zanette D., Soffientini A., Sottani C., Sarubbi E. Evaluation of phenylboronate agarose for industrial-scale purification of erythropoietin from mammalian cell cultures. J. Biotechnol. 2003, 101:275-287. 10.1016/S0168-1656(02)00357-7.