Capture of human monoclonal antibodies from a clarified cell culture supernatant by phenyl boronate chromatography

Journal of Molecular Recognition

Volumn 23, Issue 6, 2010, Pages 569-576

  Azevedo, Ana M ^a   Gomes, A G ^a   Borlido, L ^a   Santos, I F S ^a   Prazeres, D M F ^a   Aires Barros, M R ^a  

^a Instituto de Biotecnologia e Bioengenharia Lisboa   (Portugal)

Author keywords

Affinity chromatography; Capture; Downstream processing; Monoclonal antibodies; Phenyl boronate; Purification

Indexed keywords

BENZENEBORONIC ACID; BORONIC ACID DERIVATIVE; MONOCLONAL ANTIBODY;

ADSORPTION; AFFINITY CHROMATOGRAPHY; ANIMAL; ANTIBODY AFFINITY; ARTICLE; CHEMISTRY; CHO CELL; CRICETULUS; CULTURE MEDIUM; CULTURE TECHNIQUE; ENZYME SPECIFICITY; EVALUATION; FEASIBILITY STUDY; HAMSTER; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PROTEIN BINDING;

ADSORPTION; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; BORONIC ACIDS; CELL CULTURE TECHNIQUES; CHO CELLS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRICETINAE; CRICETULUS; CULTURE MEDIA, CONDITIONED; FEASIBILITY STUDIES; HUMANS; PROTEIN BINDING; SUBSTRATE SPECIFICITY;

EID: 78049449358     PISSN: 09523499     EISSN: 10991352     Source Type: Journal    
DOI: 10.1002/jmr.1068     Document Type: Article

References (23)

1. Azevedo AM, Rosa PAJ, Ferreira IF, Aires-Barros MR. 2008. Integrated process for the purification of antibodies combining aqueous two-phase extraction, hydrophobic interaction chromatography and size-exclusion chromatography. J. Chromatogr. A 1213: 154-161.
2. Azevedo AM, Rosa PAJ, Ferreira IF, Aires-Barros MR. 2009. Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing. Trends Biotechnol. 27: 240-247.
3. Bergemann K, Eckermann C, Garidel P, Grammatikos S, Jacobi A, Kaufmann H, Kempken R, Pisch-Heberle S. 2007. Production and downstream processing. In Dübel S (ed.). Handbook of Therapeutic Antibodies, Wiley: Weinheim; 199-237.
4. Bosch LI, Fyles TM, James TD. 2004. Binary and ternary phenylboronic acid complexes with saccharides and Lewis bases. Tetrahedron 60: 11175-11190.
5. Brena BM, Batista-Viera F, Rydén L, Porath J. 1992. Selective adsorption of immunoglobulins and glucosylated proteins on phenylboronate-agarose. J. Chromatogr. A 604: 109-115.
6. Curling J. 2009. The development of antibody purification technologies. In Gottschalk U (ed.). Process Scale Purification of Antibodies, Wiley: New Jersey; 25-52.
7. Dolhofer R, Wieland OH. 1980. Increased glycosylation of serum albumin in diabetes mellitus. Diabetes 29: 417-422.
8. Field R. 2007. Recombinant human IgG production frommmyeloma and Chinese hamster ovary cells. In Butler M (ed.). Cell Culture and Upstream Processing, Taylor, Francis: New York; 57-78.
9. Ghose S, McNerney T, Hubbard B. 2007. Protein A affinity chromatography for capture and purification of monoclonal antibodies and Fc-fusion proteins: practical considerations for process development. In Shukla AA, Etzel MR, Gadam S (ed.). Process Scale Bioseparations for the Biopharmaceutical Industry, Taylor, Francis: Boca Raton; 463-489.
10. Gomes AG, Azevedo AM, Aires-Barros MR, Prazeres DMF. 2010. Clearance of host cell impurities from plasmid-containing lysates by boronate adsorption. J. Chromatogr. A 1217: 2262-2266.
11. Hall DG. 2005. Structure, properties, and preparation of boronic acid derivatives. Overview of their reactions and applications. In Hall DG (ed.). Boronic Acids, Wiley: Weinheim; 1-100.
12. Hober S, Nord K, Linhult M. 2007. Protein A chromatography for antibody purification. J. Chromatogr. B 848: 40-47.
13. Kelley B, Blank G, Lee A. 2009. Downstream processing of monoclonal antibodies: current practices and future opportunities. In Gottschalk U (ed.). Process Scale Purification of Antibodies, Wiley: New Jersey; 1-23.
14. Liu X-C. 2006. Boronic acids as ligands for affinity chromatography. Chin. J. Chromatogr. 24: 73-80.
15. Low D, O'Leary R, Pujar NS. 2007. Future of antibody purification. J. Chromatogr. B 848: 48-63.
16. Przybycien TM, Pujar NS, Steele LM. 2004. Alternative bioseparation operations: life beyond packed-bed chromatography. Curr. Opin. Biotechnol. 15: 469-478.
17. Ren L, Liu Z, Dong M, Ye M, Zou H. 2009. Synthesis and characterization of a new boronate affinity monolithic capillary for specific capture of cis-diol-containing compounds. J. Chromatogr. A 1216: 4768-4774.
18. Roque ACA, Lowe CR, Taipa MA. 2004. Antibodies and genetically engineered related molecules: production and purification. Biotechnol. Prog. 20: 639-654.
19. Roque ACA, Silva CSO, Taipa MA. 2007. Affinity-based methodologies and ligands for antibody purification: advances and perspectives. J. Chromatogr. A 1160: 44-55.
20. Shukla AA, Hubbard B, Tressel T, Guhan S, Low D. 2007. Downstream processing of monoclonal antibodies - application of platform approaches. J. Chromatogr. B 848: 28-39.
21. Springsteen G, Wang B. 2002. A detailed examination of boronic acid-diol complexation. Tetrahedron 58: 5291-5300.
22. Yan J, Springsteen G, Deeter S, Wang B. 2004. The relationship among pKa, pH, and binding constants in the interactions between boronic acids and diols - it is not as simple as it appears. Tetrahedron 60: 11205-11209.
23. Zhang B, Mathewson S, Chen H. 2009. Two-dimensional liquid chromatographic methods to examine phenylboronate interactions with recombinant antibodies. J. Chromatogr. A 1216: 5676-5686.