Studies on the adsorption of cell impurities from plasmid-containing lysates to phenyl boronic acid chromatographic beads

Journal of Chromatography A

Volumn 1218, Issue 48, 2011, Pages 8629-8637

  Gomes, A Gabriela ^a   Azevedo, Ana M ^a   Aires Barros, M Raquel ^a   Prazeres, D Miguel F ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

Author keywords

Affinity chromatography; Charge transfer interactions; Genomic DNA; Phenyl boronic acid; Plasmid DNA; RNA

Indexed keywords

ACIDIC PH; BORON ATOM; BORONATE; BORONIC ACID; BUFFER COMPOSITION; CHARGE TRANSFER INTERACTION; CHARGE TRANSFER INTERACTIONS; CIS-DIOL; CONTROLLED PORE GLASS; ELECTRON DONORS; ESCHERICHIA COLI (E. COLI); GENOMIC DNA; ISO-PROPANOLS; LIPOPOLYSACCHARIDES; LYSATES; MATRIX; OLIGORIBONUCLEOTIDES; PLASMID DNA; PLASMID DNA (PDNA); POTASSIUM IONS; PRE-TREATMENT; PROTEIN REMOVAL; REMOVAL OF IMPURITIES; RNASE A; SALT CONTENT; SAMPLE PRETREATMENT; SECONDARY INTERACTIONS;

ADSORPTION; AFFINITY CHROMATOGRAPHY; ALKALINITY; BIOMOLECULES; BORON; BORON COMPOUNDS; CHARGE TRANSFER; ESCHERICHIA COLI; GENES; ION EXCHANGE; PRECIPITATION (CHEMICAL); PROTEINS; REMOVAL; RNA;

DNA;

2 PROPANOL; ACETIC ACID; BENZENEBORONIC ACID; LIPOPOLYSACCHARIDE; PLASMID DNA; POTASSIUM ION; PROTEIN; RIBONUCLEASE; RNA; SODIUM CHLORIDE;

ADSORPTION; ALKALINITY; ARTICLE; CELL LYSATE; CHEMICAL COMPOSITION; CHEMICAL INTERACTION; CHROMATOGRAPHY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; COVALENT BOND; DNA PURIFICATION; ESCHERICHIA COLI; NONHUMAN; PH; PLASMID; PRECIPITATION; PRIORITY JOURNAL;

ADSORPTION; BORONIC ACIDS; CELLS, CULTURED; CHROMATOGRAPHY, AFFINITY; DNA; GLASS; HYDROGEN-ION CONCENTRATION; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; PLASMIDS;

ESCHERICHIA COLI;

EID: 80455131119     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2011.10.004     Document Type: Article

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