Comparison of binding capacity and affinity of monoclonal antibody towards different affinity resins using high-throughput chromatography method

Journal of Applied Sciences

Volumn 12, Issue 11, 2012, Pages 1136-1141

  Pakiman, N ^a,b   Isa, N H ^a   Abol Hassan, M A ^a   Walter, J K ^a   Abdullah, N ^b  

^a Inno Biologics Sdn Bhd ^*  (Malaysia)

^b UNIVERSITY PUTRA MALAYSIA   (Malaysia)

Author keywords

Adsorption isotherm; Affinity resin; High throughput chromatography; Monoclonal antibody

Indexed keywords

ADSORPTION ISOTHERMS; AFFINITY CHROMATOGRAPHY; COLUMN CHROMATOGRAPHY; LIGANDS; MONOCLONAL ANTIBODIES; THROUGHPUT;

AFFINITY LIGANDS; AFFINITY RESINS; ALKALINE RESISTANCE; BINDING CAPACITIES; DYNAMIC BINDING CAPACITY; HIGH THROUGHPUT; PROSEP-VA ULTRA; PROTEIN CONCENTRATIONS;

RESINS;

EID: 84863999864     PISSN: 18125654     EISSN: 18125662     Source Type: Journal    
DOI: 10.3923/jas.2012.1136.1141     Document Type: Article

References (12)

1. Arnold, M., H. Bittermann, B. Kalbfuss-Zimmermann, T. Neumann and K. Schmidt et al., 2011. Antibody purification by affinity chromatography based on small molecule affinity ligands identified by SPR-based screening of chemical microarrays. J. Chromatogr. A, 1218: 4649-4659.
2. Arunakumari, A. and J. Wang, 2009. Purification of Human Monoclonal Antibodies: Non-Protein A Strategies. In: Process Scale Purification of Antibodies, Gottschalk, U. (Ed.). John Wiley and Sons, New York, ISBN: 978-0-470-20962-2.
3. Coffman, J.L., J.F. Kramarczyk and B.D. Kelley, 2008. High-throughput screening of chromatographic separations: 1. Method development and column modelling. Biotechnol. Bioeng., 100: 605-618.
4. GE Healthcare, 2009. Adsorption equilibrium isotherm studies using a high-throughput method. Application note (28-9403-62 AA), High-throughput Process Development. http://www.gelifesciences.com/aptrix/upp00919.nsf/Content/E08E8523054FCEECC1257628001D5795/$file/28940362AA.pdf.
5. Hahn, R., K. Shimahara, F. Steindl and A. Jungbauer, 2006. Comparison of affinity sorbents: III. Life time study. J. Chromatogr. A, 1102: 224-231.
6. Hahn, R., P. Bauerhansl, K. Shimahara, C. Wizniewski, A. Tscheliessnig and A. Jungbauer, 2005. Comparison of affinity sorbents: II. Mass transfer properties. J. Chromatogr. A, 1093: 98-110.
7. Hahn, R., R. Schlegel and A. Jungbauer, 2003. Comparison of protein A affinity sorbent. J. Chromatogr. B, 790: 35-51.
8. Hober, S., K. Nord and M. Linhult, 2007. Protein-A chromatography for antibody purification. J. Chromatogr. B, 848: 40-47.
9. Lacki, K.M. and E. Brekkan, 2011. High Throughput Screening Techniques in Protein Purification. In: Protein Purification Principles, High Resolution Methods and Applications, Janson, J.C. (Ed.). John Wiley and Sons, New York, ISBN: 978-0-471-74661-4.
10. Shukla, A.A., B. Hubbard, T. Tressel, S. Guhan and D. Low, 2007. Downstream processing of monoclonal antibodies-application of platform approaches. J. Chromatogr. B, 848: 28-39.
11. Swinnen, K., A. Krul, I. van Goidsenhoven, N. van Tichelt, A. Roosen and K. van Houdt, 2007. Performance comparison of protein a affinity resins for the purification of monoclonal antibodies. J. Chromatogr. B, 848: 97-107.
12. Vunnum, S., G. Vedantham and B. Hubbard, 2009. Protein-A Based Affinity Chromatography. In: Process Scale Purification of Antibodies, Gottschalk, U. (Ed.). John Wiley and Sons, New York.