Rapid molecular detection and genotyping of West Nile Virus lineages 1 and 2 by real time PCR and melting curve analysis

Journal of Virological Methods

Volumn 207, Issue , 2014, Pages 54-59

  Faggioni, Giovanni ^a   De Santis, Riccardo ^a   Pomponi, Alice ^a   Fantini, Massimo ^b   Savini, Giovanni ^c   Monaco, Federica ^c   Polci, Andrea ^c   Bei, Roberto ^b   Lista, Florigio ^a  

^a Army Medical Research Center   (Italy)

^b UNIVERSITY OF ROME TOR VERGATA   (Italy)

^c ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELL ABRUZZO E DEL MOLISE G CAPORALE   (Italy)

Author keywords

Flavivirus; Melting curve analysis; Molecular detection; Virus genotyping; WNV

Indexed keywords

ARTICLE; COMPUTER MODEL; CONSENSUS SEQUENCE; CONTROLLED STUDY; DENGUE VIRUS; GENE AMPLIFICATION; GENOTYPE; MELTING CURVE ANALYSIS; NONHUMAN; NUCLEIC ACID ANALYSIS; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; SENSITIVITY ANALYSIS; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; VIRUS DETECTION; VIRUS GENOME; WEST NILE FLAVIVIRUS; CLASSIFICATION; EUROPE; GENETICS; GENOTYPING TECHNIQUE; ISOLATION AND PURIFICATION; OLIGONUCLEOTIDE PROBE; PROCEDURES; TRANSITION TEMPERATURE; UNITED STATES; VIROLOGY; WEST NILE FEVER; WEST NILE VIRUS;

OLIGONUCLEOTIDE PROBE; PRIMER DNA; VIRUS RNA;

DNA PRIMERS; EUROPE; GENOME, VIRAL; GENOTYPING TECHNIQUES; OLIGONUCLEOTIDE PROBES; REAL-TIME POLYMERASE CHAIN REACTION; RNA, VIRAL; TRANSITION TEMPERATURE; UNITED STATES; WEST NILE FEVER; WEST NILE VIRUS;

EID: 84904269845     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2014.06.020     Document Type: Article

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