Genome sequencing of West Nile Virus from human cases in Greece, 2012

Viruses

Volumn 5, Issue 9, 2013, Pages 2311-2319

  Barzon, Luisa ^a,b   Papa, Anna ^c   Pacenti, Monia ^b   Franchin, Elisa ^a,b   Lavezzo, Enrico ^a   Squarzon, Laura ^a   Masi, Giulia ^a   Martello, Thomas ^a   Testa, Theodolinta ^c   Cusinato, Riccardo ^b   Palù, Giorgio ^a,b  

^a UNIVERSITY OF PADOVA   (Italy)

^b UNIVERSITY HOSPITAL OF PADOVA   (Italy)

^c ARISTOTLE UNIVERSITY OF THESSALONIKI   (Greece)

Author keywords

Genome sequencing; Greece; Human surveillance; Neuroinvasive disease; Phylogenetic analysis; West Nile virus

Indexed keywords

VIRUS RNA;

AGED; AMINO ACID SUBSTITUTION; ARTICLE; BLOOD SAMPLING; CLINICAL ARTICLE; CONTROLLED STUDY; EPIDEMIC; FEMALE; GENETIC ANALYSIS; GENOME ANALYSIS; GREECE; HUMAN; MOLECULAR PHYLOGENY; NEUROLOGIC DISEASE; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; NUCLEOTIDE SEQUENCE; RETROSPECTIVE STUDY; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; STRAIN DIFFERENCE; URINALYSIS; VIRUS DETECTION; VIRUS GENOME; VIRUS STRAIN; VIRUS VIRULENCE; WEST NILE FEVER; WEST NILE FLAVIVIRUS;

AGED; DISEASE OUTBREAKS; FEMALE; GENOME, VIRAL; GREECE; HUMANS; MOLECULAR SEQUENCE DATA; PHYLOGENY; VIRAL PROTEINS; WEST NILE FEVER; WEST NILE VIRUS;

EID: 84884632075     PISSN: 19994915     EISSN: None     Source Type: Journal    
DOI: 10.3390/v5092311     Document Type: Article

References (39)

1. Ulbert, S. West Nile virus: The complex biology of an emerging pathogen. Intervirology 2011, 54, 171-184.
2. Zeller, H.G.; Schuffenecker, I. West Nile Virus: An overview of its spread in Europe and the Mediterranean basin in contrast to its spread in the Americas. Eur. J. Clin. Microbiol. Infect. Dis. 2004, 23, 147-156.
3. Tsai, T.F.; Popovici, F.; Cernescu, C.; Campbell, G.L.; Nedelcu, N.I. West Nile encephalitis epidemic in southeastern Romania. Lancet 1998, 352, 767-771.
4. Lvov, D.K.; Butenko, A.M.; Gromashevsky, V.L.; Larichev, V.P.; Gaidamovich, S.Y.; Vyshemirsky, O.I.; Zhukov, A.N.; Lazorenko, V.V.; Salko, V.N.; Kovtunov, A.I.; et al. Isolation of two strains of West Nile virus during an outbreak in southern Russia, 1999. Emerg. Infect. Dis. 2000, 6, 373-376.
5. Platonov, A.E.; Shipulin, G.A.; Shipulina, O.Y.; Tyutyunnik, E.N.; Frolochkina, T.I.; Lanciotti, R.S.; Yazyshina, S.; Platonova, O.V.; Obukhov, I.L.; Zhukov, A.N.; et al. Outbreak of West Nile virus infection, Volgograd Region, Russia, 1999. Emerg. Infect. Dis. 2001, 7, 128-132.
6. Lanciotti, R.S.; Roehrig, J.T.; Deubel, V.; Smith, J.; Parker, M.; Steele, K.; Crise, B.; Volpe, K.E.; Crabtree, M.B.; Scherret, J.H.; et al. Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United States. Science 1999, 286, 2333-2337.
7. Petersen, L.R.; Brault, A.C.; Nasci, R.S. West Nile virus: Review of the literature. JAMA 2013, 310, 308-315.
8. Sambri, V.; Capobianchi, M.; Charrel, R.; Fyodorova, M.; Gaibani, P.; Gould, E.; Niedrig, M.; Papa, A.; Pierro, A.; Rossini, G.; et al. West Nile virus in Europe: emergence, epidemiology, diagnosis, treatment, and prevention. Clin. Microbiol. Infect. 2013, 19, 699-704.
9. Hellenic Centre for Disease Control and Prevention (HCDCP): West Nile virus Revised Epidemic Report; 2010. Available at: http://www.keelpno.gr/ (accessed on 1 September 2013).
10. Danis, K.; Papa, A.; Theocharopoulos, G.; Dougas, G.; Athanasiou, M.; Detsis, M.; Baka, A.; Lytras, T.; Mellou, K.; Bonovas, S.; Panagiotopoulos, T. Outbreak of West Nile virus infection in Greece, 2010. Emerg. Infect. Dis. 2011, 17, 1868-1872.
11. Papa, A. West Nile virus infections in humans-Focus on Greece. J. Clin. Virol. pii: S1386-6532(13)00081-4, doi:10.1016/j.jcv.2013.02.020.
12. Anastasiadou, A.; Economopoulou, A.; Kakoulidis, I.; Zilidou, R.; Butel, D.; Zorpidou, D.; Ferentinos, G.; Markou, P.; Kougas, E.; Papa, A. Non-neuroinvasive West Nile virus infections during the outbreak in Greece. Clin. Microbiol. Infect. 2011, 17, 1681-1683.
13. Danis, K.; Papa, A.; Papanikolaou, E.; Dougas, G.; Terzaki, I; Baka, A.; Vrioni, G.; Kapsimali, V.; Tsakris, A.; Kansouzidou, A.; et al. Ongoing outbreak of West Nile virus infection in humans, Greece, July to August 2011. Euro Surveill. 2011, 16, pii: 19951.
14. Papa, A.; Bakonyi, T.; Xanthopoulou, K.; Vázquez, A.; Tenorio, A.; Nowotny, N. Genetic characterization of West Nile virus lineage 2, Greece, 2010. Emerg. Infect. Dis. 2011, 17, 920-922.
15. Bakonyi, T.; Ivanics, E.; Erdelyi, K.; Ursu, K.; Ferenczi, E.; Weissenböck, H.; Nowotny, N. Lineage 1 and 2 strains of encephalitic West Nile virus, central Europe. Emerg. Infect. Dis. 2006, 12, 618-623.
16. Botha, E.M.; Markotter, W.; Wolfaardt, M.; Paweska, J.T.; Swanepoel, R.; Palacios, G.; Nel, L.H.; Venter, M. Genetic determinants of virulence in pathogenic lineage 2 West Nile virus strains. Emerg. Infect. Dis. 2008, 14, 222-230.
17. Venter, M.; Swanepoel, R. West Nile virus lineage 2 as a cause of zoonotic neurological disease in human and horses in southern Africa. Vector Borne Zoonotic Dis. 2010, 10, 659-664.
18. Papa, A.; Xanthopoulou, K.; Gewehr, S.; Mourelatos, S. Detection of West Nile virus lineage 2 in mosquitoes during a human outbreak in Greece. Clin. Microbiol. Infect. 2011, 17, 1176-1180.
19. Papa, A.; Papadopoulou, E.; Gavana, E.; Kalaitzopoulou, S.; Mourelatos, S. Detection of West Nile virus lineage 2 in Culex mosquitoes, Greece, 2012. Vector Borne Zoonotic Dis. 2013, doi:10.1089/vbz.2012.1212.
20. Chaskopoulou, A.; Dovas, C.I.; Chaintoutis, S.C.; Kashefi, J.; Koehler, P.; Papanastassopoulou, M. Detection and early warning of West Nile cirus circulation in Central Macedonia, Greece, using sentinel chickens and mosquitoes. Vector Borne Zoonotic Dis. 2013, doi:10.1089/vbz.2012.1176.
21. Papa, A.; Xanthopoulou, K.; Tsioka, A.; Kalaitzopoulou, S.; Mourelatos, S. West Nile virus in mosquitoes in Greece. Parasitol. Res. 2013, 112, 1551-1555.
22. Cnops, L.; Papa, A.; Lagra, F.; Weyers, P.; Meersman, K.; Patsouros, N.; Van Esbroeck, M. West Nile virus infection in Belgian traveler returning from Greece. Emerg. Infect. Dis. 2013, 19, 684-685.
23. Valiakos, G.; Touloudi, A.; Athanasiou, L.V.; Giannakopoulos, A.; Iacovakis, C.; Birtsas, P.; Spyrou, V.; Dalabiras, Z.; Petrovska, L.; Billinis, C. Serological and molecular investigation into the role of wild birds in the epidemiology of West Nile virus in Greece. Virol. J. 2012, 9, 266.
24. Valiakos, G.; Touloudi, A.; Iacovakis, C.; Athanasiou, L.; Birtsas, P.; Spyrou, V.; Billinis, C. Molecular detection and phylogenetic analysis of West Nile virus lineage 2 in sedentary wild birds (Eurasian magpie), Greece, 2010. Euro Surveill. 2011, 16, pii: 19862.
25. Chaskopoulou, A.; Dovas, C.; Chaintoutis, S.; Bouzalas, I.; Ara, G.; Papanastassopoulou, M. Evidence of enzootic circulation of West Nile virus (Nea Santa-Greece-2010, lineage 2), Greece, May to July 2011. Euro Surveill. 2011, 16, pii: 19933.
26. Chaintoutis, S.C.; Chaskopoulou, A.; Chassalevris, T.; Koehler, P.G.; Papanastassopoulou, M.; Dovas, C.I. West Nile virus lineage 2 strain in Greece, 2012. Emerg. Infect. Dis. 19, 827-829.
27. Ciccozzi, M.; Peletto, S.; Cella, E.; Giovanetti, M.; Lai, A.; Gabanelli, E.; Acutis, P.L.; Modesto, P.; Rezza, G.; Platonov, A.E.; Lo Presti, A.; Zehender, G. Epidemiological history and phylogeography of West Nile virus lineage 2. Infect. Genet. Evol. 2013, 17, 46-50.
28. McMullen, A.R.; Albayrak, H.; May, F.J.; Davis, C.T.; Beasley, D.W.; Barrett, A.D. Molecular evolution of lineage 2 West Nile virus. J. Gen. Virol. 2013, 94, 318-325.
29. Brault, A.C.; Huang, C.Y.; Langevin, S.A.; Kinney, R.M.; Bowen, R.A.; Ramey, W.N.; Panella, N.A.; Holmes, E.C.; Powers, A.M.; Miller, B.R. A single positively selected West Nile viral mutation confers increased virogenesis in American crows. Nat. Genet. 2007, 39, 1162-1166.
30. Kantzanou, M.N.; Moschidis, Z.M.; Kremastinou, G.; Levidiotou, S.; Karafoulidou, A.; Politis, C.; Marantidou, O.; Kavallierou, L.; Kaperoni, A.; Veneti, C.; et al. Searching for West Nile virus (WNV) in Greece. Transfus Med 2010, 20, 113-117.
31. Dimou, V.; Gerou, S.; Papa, A. The epidemic West Nile virus strain in Greece was a recent introduction. Vector Borne Zoonotic Dis 2013, doi:10.1089/vbz.2013.1318.
32. Kimura, M. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol.1980, 16, 111-120.
33. Felsenstein, J. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 1985, 39, 783-791.
34. Tamura, K.; Peterson, D.; Peterson, N.; Stecher, G.; Nei, M.; Kumar, S. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 2011, 28, 2731-2739.
35. Barzon, L.; Pacenti, M.; Franchin, E.; Pagni, S.; Martello, T.; Cattai, M.; Cusinato, R.; Palù, G. Excretion of West Nile virus in urine during acute infection. J. Infect. Dis. 2013, doi:10.1093/infdis/jit290.
36. Lanciotti, R.S.; Kerst, A.J.; Nasci, R.S.; Godsey, M.S.; Mitchell, C.J.; Savage, H.M.; Komar, N.; Panella, N.A.; Allen, B.C.; Volpe, K.E.; Davis, B.S.; Roehrig, J.T. Rapid detection of West Nile virus from human clinical specimens, field-collected mosquitoes, and avian samples by a TaqMan reverse transcriptase-PCR assay. J. Clin. Microbiol. 2000, 38, 4066-4071.
37. Linke, S.; Ellerbrok, H.; Niedrig, M.; Nitsche, A.; Pauli, G. Detection of West Nile virus lineages 1 and 2 by real-time PCR. J. Virol. Methods. 2007, 146, 355-358.
38. Scaramozzino, N.; Crance, J.M.; Jouan, A.; DeBriel, D.A.; Stoll, F.; Garin, D. Comparison of flavivirus universal primer pairs and development of a rapid, highly sensitive heminested reverse transcription-PCR assay for detection of flaviviruses targeted to a conserved region of the NS5 gene sequences. J.Clin. Microbiol. 2001, 39, 1922-1927.
39. Barzon, L.; Pacenti, M.; Cusinato, R.; Cattai, M.; Franchin, E.; Pagni, S.; Martello, T.; Bressan, S.; Squarzon, L.; Cattelan, A.; et al. Human cases of West Nile Virus Infection in north-eastern Italy, 15 June to 15 November 2010. Euro Surveill. 2011, 16, pii: 19949.