Decreasing the amount of trypsin in in-gel digestion leads to diminished chemical noise and improved protein identifications

Journal of Proteomics

Volumn 109, Issue , 2014, Pages 16-25

  Hu, Mo ^a   Liu, Yanhua ^a   Yu, Kaiwen ^a   Liu, Xiaoyun ^a  

^a PEKING UNIVERSITY   (China)

Author keywords

Chemical noise; In gel digestion; Mass spectrometry; Proteomics; Trypsin autolysis

Indexed keywords

DEFERIPRONE; KERATIN; TRYPSIN; BACTERIAL PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; AUTOLYSIS; CELL LYSATE; CHEMICAL NOISE; COLLISIONALLY ACTIVATED DISSOCIATION; CONTAMINATION; COST; DIGESTION; GEL; GEL ELECTROPHORESIS; IN GEL DIGESTION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METHYLATION; NOISE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEOMICS; SALMONELLA TYPHIMURIUM; STATIC ELECTRICITY; TANDEM MASS SPECTROMETRY; WORK ENVIRONMENT; CHEMISTRY; METABOLISM; PROCEDURES; PROTEIN DEGRADATION; SALMONELLA ENTERICA SEROVAR TYPHIMURIUM; SIGNAL NOISE RATIO; TWO DIMENSIONAL GEL ELECTROPHORESIS; DIFFUSION;

BACTERIAL PROTEINS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; PROTEOLYSIS; PROTEOMICS; SALMONELLA TYPHIMURIUM; SIGNAL-TO-NOISE RATIO; TRYPSIN;

EID: 84904089086     PISSN: 18743919     EISSN: 18767737     Source Type: Journal    
DOI: 10.1016/j.jprot.2014.06.017     Document Type: Article

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