Usage of electrostatic eliminator reduces human keratin contamination significantly in gel-based proteomics analysis

Journal of Proteomics

Volumn 74, Issue 7, 2011, Pages 1022-1029

  Xu, Bo ^a   Zhang, Ying ^a   Zhao, Zongjiang ^a   Yoshida, Yutaka ^a   Magdeldin, Sameh ^a,b   Fujinaka, Hidehiko ^a,c   Ismail, Tamer Ahmed ^a,d   Yaoita, Eishin ^a   Yamamoto, Tadashi ^a  

^a NIIGATA UNIVERSITY GRADUATE SCHOOL OF MEDICAL AND DENTAL SCIENCES   (Japan)

^b Suez Canal University   (Egypt)

^c Niigata National Hospital   (Japan)

^d Zagazig University   (Egypt)

Author keywords

In gel digestion; Keratin contamination; Mass spectrometry; Static electricity eliminator

Indexed keywords

KERATIN;

ARTICLE; CONTROLLED STUDY; GEL ELECTROPHORESIS; HUMAN; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEOMICS; RAT; STATIC ELECTRICITY;

ANIMALS; DRUG CONTAMINATION; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HUMANS; KERATINS; PROTEINS; PROTEOMICS; RATS; STAINING AND LABELING; STATIC ELECTRICITY;

EID: 79957668546     PISSN: 18743919     EISSN: 18767737     Source Type: Journal    
DOI: 10.1016/j.jprot.2011.03.001     Document Type: Article

References (12)

1. Capelo J.L., Carreira R., Diniz M., Fernandes L., Galesio M., Lodeiro C., Santos H.M., Vale G. Overview on modern approaches to speed up protein identification workflows relying on enzymatic cleavage and mass spectrometry-based techniques. Anal Chim Acta 2009 Sep 21, 650(2):151-159.
2. Granvogl B., PlÖscher M., Eichacker L.A. Sample preparation by in-gel digestion for mass spectrometry-based proteomics. Anal Bioanal Chem 2007 Oct, 389(4):991-1002.
3. Ding Q., Xiao L., Xiong S., Jia Y., Que H., Guo Y., Liu S. Unmatched masses in peptide mass fingerprints caused by cross-contamination: an updated statistical result. Proteomics 2003 Jul, 3(7):1313-1317.
4. Thurgood L.A., Ryall R.L. Proteomic analysis of proteins selectively associated with hydroxyapatite, brushite, and uric acid crystals precipitated from human urine. J Proteome Res 2010 Oct 1, 9(10):5402-5412.
5. Stamenkovic I., Skalli O., Gabbiani G. Distribution of intermediate filament proteins in normal and diseased human glomeruli. Am J Pathol 1986 Dec, 125(3):465-475.
6. Fleming S., Symes C.E. The distribution of cytokeratin antigens in the kidney and in renal tumours. Histopathology 1987 Feb, 11(2):157-170.
7. Tsugita A., Kawakami T., Uchida T., Sakai T., Kamo M., Matsui T., Watanabe Y., Morimasa T., Hosokawa K., Toda T. Proteome analysis of mouse brain: two-dimensional electrophoresis profiles of tissue proteins during the course of aging. Electrophoresis 2000 May, 21(9):1853-1871.
8. Zhang Y., Yoshida Y., Nameta M., Xu B., Taguchi I., Ikeda T., Fujinaka H., Mohamed S.M., Tsukaguchi H., Harita Y., Yaoita E., Yamamoto T. Glomerular proteins related to slit diaphragm and matrix adhesion in the foot processes are highly tyrosine phosphorylated in the normal rat kidney. Nephrol Dial Transplant 2010 Jun, 25(6):1785-1795.
9. Xu B., Yoshida Y., Zhang Y., Yaoita E., et al. Two-dimensional electrophoretic profiling of normal human kidney: differential protein expression in glomerulus, cortex and medulla. J Electrophoresis 2005, 49(1):5-13.
10. Jin H., Ma K.D., Hu R., Chen Y., Yang F., Yao J., Li X.T., Yang P.Y. Analysis of expression and comparative profile of normal placental tissue proteins and those in preeclampsia patients using proteomic approaches. Anal Chim Acta 2008 Nov 23, 629(1-2):158-164.
11. Griffin N.M., Yu J., Long F., Oh P., Shore S., Li Y., Koziol J.A., Schnitzer J.E. Label-free, normalized quantification of complex mass spectrometry data for proteomic analysis. Nat Biotechnol 2010 Jan, 28(1):83-89. Epub 2009 Dec 13.
12. Alvarado R., Tran D., Ching B., Phinney B.S. A comparative study of in-gel digestions using microwave and pressure-accelerated technologies. J Biomol Tech 2010 Sep, 21(3):148-155.