Aptamer binding assays for proteins: The thrombin example-A review

Analytica Chimica Acta

Volumn 837, Issue , 2014, Pages 1-15

  Deng, Bin ^a   Lin, Yanwen ^a   Wang, Chuan ^a   Li, Feng ^a   Wang, Zhixin ^a   Zhang, Hongquan ^a   Li, Xing Fang ^a   Le, X Chris ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Affinity separation; Aptamers; Binding induced DNA assembly; Catalytic amplification; Homogeneous binding assays; Thrombin

Indexed keywords

DNA;

AFFINITY SEPARATION; APTAMERS; BINDING ASSAYS; DNA ASSEMBLIES; THROMBIN;

ENZYMES;

APTAMER; NANOMATERIAL; PLATINUM NANOPARTICLE; QUANTUM DOT; RIBOZYME; THROMBIN; PROTEIN BINDING;

AFFINITY CHROMATOGRAPHY; BINDING ASSAY; BIOSENSOR; CAPILLARY ELECTROPHORESIS; CHROMATOGRAPHY; DNA CLEAVAGE; DNA SEQUENCE; ELECTROCHEMICAL APTASENSOR; ELECTROCHEMISTRY; ENZYME ACTIVITY; ENZYME SPECIFICITY; FLUORESCENCE RESONANCE ENERGY TRANSFER; LIGAND BINDING; LIMIT OF DETECTION; MICROFLUIDICS; PHOTOLUMINESCENCE; PRIORITY JOURNAL; PROTEIN BINDING; REVIEW; RNA BINDING; RNA SEQUENCE; SEPARATION TECHNIQUE; SURFACE PROPERTY; CHEMISTRY; ELECTROCHEMICAL ANALYSIS; FRACTIONATION; PROCEDURES;

APTAMERS, NUCLEOTIDE; CHEMICAL FRACTIONATION; ELECTROCHEMICAL TECHNIQUES; PROTEIN BINDING; THROMBIN;

EID: 84903819956     PISSN: 00032670     EISSN: 18734324     Source Type: Journal    
DOI: 10.1016/j.aca.2014.04.055     Document Type: Review

References (120)

1. Ellington A.D., Szostak J.W. In vitro selection of RNA molecules that bind specific ligands. Nature 1990, 346:818-822.
2. Tuerk C., Gold L. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science 1990, 249:505-510.
3. Hamula C.L.A., Guthrie J., Zhang H., Li X.-F., Le X.C. Selection and analytical applications of aptamers. Trend. Anal. Chem. 2006, 25:681-691.
4. Mayer G. The chemical biology of aptamers. Angew. Chem. Int. Ed. 2009, 48:2672-2689.
5. Stoltenburg R., Reinemann C., Strehlitz B. SELEX - a (r)evolutionary method to generate high-affinity nucleic acid ligands. Biomol. Eng. 2007, 24:381-403.
6. Shamah S.M., Healy J.M., Cload S.T. Complex target SELEX. Acc. Chem. Res. 2008, 41:130-138.
7. Fang X., Tan W. Aptamers generated from cell-SELEX for molecular medicine: A chemical biology approach. Acc. Chem. Res. 2010, 43:48-57.
8. Keefe A.D., Pai S., Ellington A. Aptamers as therapeutics. Nat. Rev. Drug Discov. 2010, 9:537-550.
9. Hamula C.L.A., Zhang H., Li F., Wang Z., Le X.C., Li X.-F. Selection and analytical applications of aptamers binding microbial pathogens. Trend. Anal. Chem. 2011, 30:1587-1597.
10. McKeague M., Derosa M.C. Challenges and opportunities for small molecule aptamer development. J. Nucleic Acids 2012, 2012:1-21.
11. Jayasena S.D. Aptamers: an emerging class of molecules that rival antibodies in diagnostics. Clin. Chem. 1999, 45:1628-1650.
12. Huntington J.A. Molecular recognition mechanisms of thrombin. J. Thromb. Haemost. 2005, 3:1861-1872.
13. Davie E.W., Kulman J.D. An overview of the structure and function of thrombin. Semin. Thromb. Hemost. 2006, 32(Suppl. 1):3-15.
14. Crawley J.T., Zanardelli S., Chion C.K., Lane D.A. The central role of thrombin in hemostasis. J. Thromb. Haemost. 2007, 5(Suppl. 1):95-101.
15. Di Cera E. Thrombin. Mol. Aspects Med. 2008, 29:203-254.
16. Sokolova E., Reiser G. Prothrombin/thrombin and the thrombin receptors PAR-1 and PAR-4 in the brain: localization, expression and participation in neurodegenerative diseases. Thromb. Haemost. 2008, 100:576-581.
17. Franchini M., Mannucci P.M. Thrombin and cancer: from molecular basis to therapeutic implications. Semin. Thromb. Hemost. 2012, 38:95-101.
18. Lawson J.H. The clinical use and immunologic impact of thrombin in surgery. Semin. Thromb. Hemost. 2006, 32(Suppl. 1):98-110.
19. Shuman M.A., Majerus P.W. The measurement of thrombin in clotting blood by radioimmunoassay. J. Clin. Invest. 1976, 58:1249-1258.
20. Brummel-Ziedins K.E., Vossen C.Y., Butenas S., Mann K.G., Rosendaal F.R. Thrombin generation profiles in deep venous thrombosis. J. Thromb. Haemost. 2005, 3:2497-2505.
21. Macfarlane R.G., Biggs R. A thrombin generation test; the application in haemophilia and thrombocytopenia. J. Clin. Pathol. 1953, 6:3-8.
22. Luddington R., Baglin T. Clinical measurement of thrombin generation by calibrated automated thrombography requires contact factor inhibition. J. Thromb. Haemost. 2004, 2:1954-1959.
23. Rand M.D., Lock J.B., van't Veer C., Gaffney D.P., Mann K.G. Blood clotting in minimally altered whole blood. Blood 1996, 88:3432-3445.
24. Soslau G., Goldenberg S.J., Class R., Jameson B. Differential activation and inhibition of human platelet thrombin receptors by structurally distinct alpha-, beta- and gamma-thrombin. Platelets 2004, 15:155-166.
25. Lundblad R.L., Nesheim M.E., Straight D.L., Sailor S., Bowie J., Jenzano J.W., Roberts J.D., Mann K.G. Bovine alpha- and beta-thrombin. Reduced fibrinogen-clotting activity of beta-thrombin is not a consequence of reduced affinity for fibrinogen. J. Biol. Chem. 1984, 259:6991-6995.
26. Takahashi K., Aiken M., Fenton J.W., Walz D.A. Thrombospondin fragmentation by alpha-thrombin and resistance to gamma-thrombin. Biochem. J. 1984, 224:673-676.
27. Bock L.C., Griffin C., Vermaas E.H., Toole J.J. Selection of single-stranded DNA molecules that bind and inhibit human thrombin. Nature 1992, 355:564-566.
28. Macaya R.F., Schultze P., Smith F.W., Roe J.A., Feigon J. Thrombin-binding DNA aptamer forms a unimolecular quadruplex structure in solution. Proc. Natl. Aca. Sci. U. S. A. 1993, 90:3745-3749.
29. Tasset D.M., Kubik M.F., Steiner W. Oligonucleotide inhibitors of human thrombin that bind distinct epitopes. J. Mol. Biol. 1997, 272:688-698.
30. Kong D.M., Xu J., Shen H.X. Positive effects of ATP on G-quadruplex-hemin DNAzyme-mediated reactions. Anal. Chem. 2010, 82:6148-6153.
31. Tyagi S., Kramer F.R. Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol. 1996, 14:303-308.
32. Li J.J., Fang X., Schuster S.M., Tan W. Molecular beacons: a novel approach to detect protein-DNA interactions. Angew. Chem.-Int. Ed. 2000, 39:1049-1052.
33. Hamaguchi N., Ellington A., Stanton M. Aptamer beacons for the direct detection of proteins. Anal. Biochem. 2001, 294:126-131.
34. Li J.J., Fang X., Tan W. Molecular aptamer beacons for real-time protein recognition. Biochem. Biophys. Res. Commun. 2002, 292:31-40.
35. Nutiu R., Li Y. Structure-switching signaling aptamers. J. Am. Chem. Soc. 2003, 125:4771-4778.
36. Levy M., Cater S.F., Ellington A.D. Quantum-dot aptamer beacons for the detection of proteins. Chembiochem: A Eur. J. Chem. Biol. 2005, 6:2163-2166.
37. Wang W., Chen C., Qian M., Zhao X.S. Aptamer biosensor for protein detection using gold nanoparticles. Anal. Biochem. 2008, 373:213-219.
38. Li N., Ho C.M. Aptamer-based optical probes with separated molecular recognition and signal transduction modules. J. Am. Chem. Soc. 2008, 130:2380-2381.
39. Li T., Wang E., Dong S. G-quadruplex-based DNAzyme for facile colorimetric detection of thrombin. Chem. Commun. 2008, 31:3654-3656.
40. Zhang L., Zhu J., Li T., Wang E. Bifunctional colorimetric oligonucleotide probe based on a G-quadruplex DNAzyme molecular beacon. Anal. Chem. 2011, 83:8871-8876.
41. Zhang Y., Li B., Jin Y. Label-free fluorescent detection of thrombin using G-quadruplex-based DNAzyme as sensing platform. Analyst 2011, 136:3268-3273.
42. Liu X., Freeman R., Golub E., Willner I. Chemiluminescence and chemiluminescence resonance energy transfer (CRET) aptamer sensors using catalytic hemin/G-quadruplexes. ACS Nano 2011, 5:7648-7655.
43. Najafi-Shoushtari S.H., Famulok M. DNA aptamer-mediated regulation of the hairpin ribozyme by human alpha-thrombin. Blood Cells Mol. Dis. 2007, 38:19-24.
44. Cui L., Ke G., Wang C., Yang C.J. A cyclic enzymatic amplification method for sensitive and selective detection of nucleic acids. Analyst 2010, 135:2069-2073.
45. Li J., Fu H.E., Wu L.J., Zheng A.X., Chen G.N., Yang H.H. General colorimetric detection of proteins and small molecules based on cyclic enzymatic signal amplification and hairpin aptamer probe. Anal. Chem. 2012, 84:5309-5315.
46. Xue L., Zhou X., Xing D. Sensitive and homogeneous protein detection based on target-triggered aptamer hairpin switch and nicking enzyme assisted fluorescence signal amplification. Anal. Chem. 2012, 84:3507-3513.
47. Xue L., Zhou X., Xing D. Highly sensitive protein detection based on aptamer probe and isothermal nicking enzyme assisted fluorescence signal amplification. Chem. Commun. 2010, 46:7373-7375.
48. Zheng D., Zou R., Lou X. Label-free fluorescent detection of ions, proteins, and small molecules using structure-switching aptamers, SYBR Gold, and exonuclease I. Anal. Chem. 2012, 84:3554-3560.
49. Wang X.L., Li F., Su Y.H., Sun X., Li X.B., Schluesener H.J., Tang F., Xu S.Q. Ultrasensitive detection of protein using an aptamer-based exonuclease protection assay. Anal. Chem. 2004, 76:5605-5610.
50. Liu X., Freeman R., Willner I. Amplified fluorescence aptamer-based sensors using exonuclease III for the regeneration of the analyte. Chemistry 2012, 18:2207-2211.
51. Yang R., Tang Z., Yan J., Kang H., Kim Y., Zhu Z., Tan W. Noncovalent assembly of carbon nanotubes and single-stranded DNA: an effective sensing platform for probing biomolecular interactions. Anal. Chem. 2008, 80:7408-7413.
52. Chang H., Tang L., Wang Y., Jiang J., Li J. Graphene fluorescence resonance energy transfer aptasensor for the thrombin detection. Anal. Chem. 2010, 82:2341-2346.
53. Zhang Y., Liu S., Sun X. Mesoporous carbon microparticles as a novel fluorescent sensing platform for thrombin detection. Biosens. Bioelectron. 2011, 26:3876-3880.
54. Zhang Y., Sun X. A novel fluorescent aptasensor for thrombin detection: using poly(m-phenylenediamine) rods as an effective sensing platform. Chem. Commun. 2011, 47:3927-3929.
55. Liu J., Li J., Jiang Y., Yang S., Tan W., Yang R. Combination of π-π stacking and electrostatic repulsion between carboxylic carbon nanoparticles and fluorescent oligonucleotides for rapid and sensitive detection of thrombin. Chem. Commun. 2011, 47:11321-11323.
56. Li H., Zhang Y., Luo Y., Sun X. Nano-C(60): a novel, effective, fluorescent sensing platform for biomolecular detection. Small 2011, 7:1562-1568.
57. Gill R., Polsky R., Willner I. Pt nanoparticles functionalized with nucleic acid act as catalytic labels for the chemiluminescent detection of DNA and proteins. Small 2006, 2:1037-1041.
58. Higuchi A., Siao Y.D., Yang S.T., Hsieh P.V., Fukushima H., Chang Y., Ruaan R.C., Chen W.Y. Preparation of a DNA aptamer-Pt complex and its use in the colorimetric sensing of thrombin and anti-thrombin antibodies. Anal. Chem. 2008, 80:6580-6586.
59. Choi J.H., Chen K.H., Strano M.S. Aptamer-capped nanocrystal quantum dots: a new method for label-free protein detection. J. Am. Chem. Soc. 2006, 128:15584-15585.
60. Gao L., Zhuang J., Nie L., Zhang J., Zhang Y., Gu N., Wang T., Feng J., Yang D., Perrett S., Yan X. Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nat. Nanotechnol. 2007, 2:577-583.
61. Hu P., Han L., Zhu C., Dong S.J. Nanoreactors: a novel biosensing platform for protein assay. Chem. Commun. 2013, 49:1705-1707.
62. Fredriksson S., Gullberg M., Jarvius J., Olsson C., Pietras K., Gustafsdottir S.M., Ostman A., Landegren U. Protein detection using proximity-dependent DNA ligation assays. Nat. Biotechnol. 2002, 20:473-477.
63. Heyduk E., Heyduk T. Nucleic acid-based fluorescence sensors for detecting proteins. Anal. Chem. 2005, 77:1147-1156.
64. Di Giusto D.A., Wlassoff W.A., Gooding J.J., Messerle B.A., King G.C. Proximity extension of circular DNA aptamers with real-time protein detection. Nucleic Acids Res. 2005, 33:e64.
65. X.C. Le, H. Zhang, X.-F. Li, United States patent application 61/238,368, Publication No. US 2012/0156678A1 (2012).
66. Zhang H., Li X.-F., Le X.C. Binding-induced DNA assembly and its application to yoctomole detection of proteins. Anal. Chem. 2012, 84:877-884.
67. Zhang H., Li F., Dever B., Wang C., Li X.-F., Le X.C. Assembling DNA through affinity binding to achieve ultrasensitive protein detection. Angew. Chem.-Int. Ed. 2013, 52:10698-10705.
68. Li F., Zhang H., Lai C., Li X.-F., Le X.C. A molecular translator that acts by binding-induced DNA strand displacement for a homogeneous protein assay. Angew. Chem.-Int. Ed. 2012, 51:9317-9320.
69. Li F., Zhang H., Wang Z., Li X., Li X.-F., Le X.C. Dynamic DNA assemblies mediated by binding-induced DNA strand displacement. J. Am. Chem. Soc. 2013, 135:2443-2446.
70. Zhang H., Li F., Li X.-F., Le X.C. Yoctomole detection of proteins using solid phase binding-induced DNA assembly. Methods 2013, 64:322-330.
71. Li J., Zhong X., Zhang H., Le X.C., Zhu J.J. Binding-induced fluorescence turn-on assay using aptamer-functionalized silver nanocluster DNA probes. Anal. Chem. 2012, 84:5170-5174.
72. Yeh H.C., Sharma J., Han J.J., Martinez J.S., Werner J.H. A DNA-silver nanocluster probe that fluoresces upon hybridization. Nano Lett. 2010, 10:3106-3110.
73. Willner I., Zayats M. Electronic aptamer-based sensors. Angew. Chem.-Int. Ed. 2007, 46:6408-6418.
74. Mir M., Vreeke M., Katakis I. Different strategies to develop an electrochemical thrombin aptasensor. Electrochem. Commun. 2006, 8:505-511.
75. Ikebukuro K., Kiyohara C., Sode K. Novel electrochemical sensor system for protein using the aptamers in sandwich manner. Biosens. Bioelectron. 2005, 20:2168-2172.
76. Polsky R., Gill R., Kaganovsky L., Willner I. Nucleic acid-functionalized Pt nanoparticles: catalytic labels for the amplified electrochemical detection of biomolecules. Anal. Chem. 2006, 78:2268-2271.
77. He P., Shen L., Cao Y., Li D. Ultrasensitive electrochemical detection of proteins by amplification of aptamer-nanoparticle bio bar codes. Anal. Chem. 2007, 79:8024-8029.
78. Deng C., Chen J., Nie Z., Wang M., Chu X., Chen X., Xiao X., Lei C., Yao S. Impedimetric aptasensor with femtomolar sensitivity based on the enlargement of surface-charged gold nanoparticles. Anal. Chem. 2009, 81:739-745.
79. Numnuam A., Chumbimuni-Torres K.Y., Xiang Y., Bash R., Thavarungkul P., Kanatharana P., Pretsch E., Wang J., Bakker E. Aptamer-based potentiometric measurements of proteins using ion-selective microelectrodes. Anal. Chem. 2008, 80:707-712.
80. Bai L., Yuan R., Chai Y., Yuan Y., Wang Y., Xie S. Direct electrochemistry and electrocatalysis of a glucose oxidase-functionalized bioconjugate as a trace label for ultrasensitive detection of thrombin. Chem. Commun. 2012, 48:10972-10974.
81. Bai L., Yuan R., Chai Y., Zhuo Y., Yuan Y., Wang Y. Simultaneous electrochemical detection of multiple analytes based on dual signal amplification of single-walled carbon nanotubes and multi-labeled graphene sheets. Biomaterials 2012, 33:1090-1096.
82. Xiao Y., Lubin A.A., Heeger A.J., Plaxco K.W. Label-free electronic detection of thrombin in blood serum by using an aptamer-based sensor. Angew. Chem.-Int. Ed. 2005, 44:5456-5459.
83. Xiao Y., Piorek B.D., Plaxco K.W., Heeger A.J. A reagentless signal-on architecture for electronic, aptamer-based sensors via target-induced strand displacement. J. Am. Chem. Soc. 2005, 127:17990-17991.
84. Das J., Cederquist K.B., Zaragoza A.A., Lee P.E., Sargent E.H., Kelley S.O. An ultrasensitive universal detector based on neutralizer displacement. Nat. Chem. 2012, 4:642-648.
85. Hu J., Wang T., Kim J., Shannon C., Easley C.J. Quantitation of femtomolar protein levels via direct readout with the electrochemical proximity assay. J. Am. Chem. Soc. 2012, 134:7066-7072.
86. Giese B. Long-distance electron transfer through DNA. Ann. Rev. Biochem. 2002, 71:51-70.
87. Huang Y.C., Ge B., Sen D., Yu H.Z. Immobilized DNA switches as electronic sensors for picomolar detection of plasma proteins. J. Am. Chem. Soc. 2008, 130:8023-8029.
88. Zhao Q., Li X.-F., Le X.C. Aptamer-modified monolithic capillary chromatography for protein separation and detection. Anal. Chem. 2008, 80:3915-3920.
89. Zhao Q., Le X.C. Recent advances in aptamer affinity chromatography. Chin. J. Chromatogr. 2009, 27:556-565.
90. Deng N., Liang Z., Liang Y., Sui Z., Zhang L., Wu Q., Yang K., Zhang L., Zhang Y. Aptamer modified organic-inorganic hybrid silica monolithic capillary columns for highly selective recognition of thrombin. Anal. Chem 2012, 84:10186-10190.
91. Zhao Q., Li X.-F., Shao Y., Le X.C. Aptamer-based affinity chromatographic assays for thrombin. Anal. Chem. 2008, 80:7586-7593.
92. German I., Buchanan D.D., Kennedy R.T. Aptamers as ligands in affinity probe capillary electrophoresis. Anal. Chem. 1998, 70:4540-4545.
93. Berezovski M., Nutiu R., Li Y., Krylov S.N. Affinity analysis of a protein-aptamer complex using nonequilibrium capillary electrophoresis of equilibrium mixtures. Anal. Chem. 2003, 75:1382-1386.
94. Pavski V., Le X.C. Ultrasensitive protein-DNA binding assays. Curr. Opin. Biotech. 2003, 14:65-73.
95. Le X.C., Wan Q.H., Lam M.T. Fluorescence polarization detection for affinity capillary electrophoresis. Electrophoresis 2002, 23:903-908.
96. Ostergaard J., Heegaard N.H. Bioanalytical interaction studies executed by preincubation affinity capillary electrophoresis. Electrophoresis 2006, 27:2590-2608.
97. Zhang H., Wang Z., Li X.-F., Le X.C. Ultrasensitive detection of proteins by amplification of affinity aptamers. Angew. Chem.-Int. Ed. 2006, 45:1576-1580.
98. Zhang H., Li X.-F., Le X.C. Tunable aptamer capillary electrophoresis and its application to protein analysis. J. Am. Chem. Soc. 2008, 130:34-35.
99. Li Y., Guo L., Zhang F., Zhang Z., Tang J., Xie J. High-sensitive determination of human alpha-thrombin by its 29-mer aptamer in affinity probe capillary electrophoresis. Electrophoresis 2008, 29:2570-2577.
100. Zhang Y., Song M., Li T., Sai D., Wang H. Determination of human thrombin by an aptamer based on affinity capillary electrophoresis-laser induced fluorescence detection. Chin. J. Chromatogr. 2009, 27:333-336.
101. Wan Q.H., Le X.C. Capillary electrophoresis coupled with laser-induced fluorescence polarization as a hybrid approach to ultrasensitive immunoassays. J. Chromatogr. A 1999, 853:555-562.
102. Le X.C., Wan Q.H., Lam M.T. Fluorescence polarization detection for affinity capillary electrophoresis. Electrophoresis 2002, 23:903-908.
103. Song M., Zhang Y., Li T., Wang Z., Yin J., Wang H. Highly sensitive detection of human thrombin in serum by affinity capillary electrophoresis/laser-induced fluorescence polarization using aptamers as probes. J. Chromatogr. A 2009, 1216:873-878.
104. Obubuafo A., Balamurugan S., Shadpour H., Spivak D., McCarley R.L., Soper S.A. Poly(methyl methacrylate) microchip affinity capillary gel electrophoresis of aptamer-protein complexes for the analysis of thrombin in plasma. Electrophoresis 2008, 29:3436-3445.
105. Gong M., Nikcevic I., Wehmeyer K.R., Limbach P.A., Heineman W.R. Protein-aptamer binding studies using microchip affinity capillary electrophoresis. Electrophoresis 2008, 29:1415-1422.
106. Kim Y., Cha M., Choi Y., Joo H., Lee J. Electrokinetic separation of biomolecules through multiple nano-pores on membrane. Chem. Phys. Lett. 2013, 561, 562:63-67.
107. Wang H., Liu Y., Liu C., Huang J., Yang P., Liu B. Microfluidic chip-based aptasensor for amplified electrochemical detection of human thrombin. Electrochem. Commun. 2010, 12:258-261.
108. Srinivas R.L., Chapin S.C., Doyle P.S. Aptamer-functionalized microgel particles for protein detection. Anal. Chem. 2011, 83:9138-9145.
109. Tennico Y.H., Hutanu D., Koesdjojo M.T., Bartel C.M., Remcho V.T. On-chip aptamer-based sandwich assay for thrombin detection employing magnetic beads and quantum dots. Anal. Chem. 2010, 82:5591-5597.
110. Chen Y., Nakamoto K., Niwa O., Corn R.M. On-chip synthesis of RNA aptamer microarrays for multiplexed protein biosensing with SPR imaging measurements. Langmuir 2012, 28:8281-8285.
111. Jung A., Gronewold T.M.A., Tewes M., Quandt E., Berlin P. Biofunctional structural design of SAW sensor chip surfaces in a microfluidic sensor system. Sensor. Actuat. B: Chem. 2007, 124:46-52.
112. Li W., Li J., Qiang W., Xu J., Xu D. Enzyme-free colorimetric bioassay based on gold nanoparticle-catalyzed dye decolorization. Analyst 2013, 138:760-766.
113. Szymanski M., Noble J., Knight A., Porter R., Worsley G. Aptamer-mediated detection of thrombin using silver nanoparticle signal enhancement. Anal. Methods 2013, 5:187-191.
114. Wang X., Zhao Q. A fluorescent sandwich assay for thrombin using aptamer modified magnetic beads and quantum dots. Microchim. Acta. 2012, 178:349-355.
115. Centi S., Tombelli S., Minunni M., Mascini M. Aptamer-based detection of plasma proteins by an electrochemical assay coupled to magnetic beads. Anal. Chem. 2007, 79:1466-1473.
116. Wang Y., He X., Wang K., Ni X., Su J., Chen Z. Electrochemical detection of thrombin based on aptamer and ferrocenylhexanethiol loaded silica nanocapsules. Biosens. Bioelectron. 2011, 26:3536-3541.
117. Zhao Q., Wang X. An aptamer-capture based chromogenic assay for thrombin. Biosens. Bioelectron. 2012, 34:232-237.
118. Zhao Q., Li X.-F., Le X.C. Aptamer capturing of enzymes on magnetic beads to enhance assay specificity and sensitivity. Anal. Chem. 2011, 83:9234-9236.
119. Centi S., Messina G., Tombelli S., Palchetti I., Mascini M. Different approaches for the detection of thrombin by an electrochemical aptamer-based assay coupled to magnetic beads. Biosens. Bioelectron. 2008, 23:1602-1609.
120. Hecht A., Kumar A.A., Kopelman R. Label-acquired magnetorotation as a signal transduction method for protein detection: aptamer-based detection of thrombin. Anal. Chem. 2011, 83:7123-7128.