Structure-switching signaling aptamers

Journal of the American Chemical Society

Volumn 125, Issue 16, 2003, Pages 4771-4778

  Nutiu, Razvan ^a   Li, Yingfu ^a  

^a MCMASTER UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

APTAMERS;

DISSOCIATION; FLUORESCENCE; QUENCHING; SYNTHESIS (CHEMICAL);

DNA;

APTAMER; NUCLEIC ACID; OLIGONUCLEOTIDE;

ARTICLE; CHEMICAL STRUCTURE; COMPLEX FORMATION; DNA BINDING; DNA SEQUENCE; FLUORESCENCE; PROTEIN TARGETING; SIGNAL TRANSDUCTION; STRUCTURE ANALYSIS;

ADENOSINE TRIPHOSPHATE; BIOSENSING TECHNIQUES; COLD; DNA; DNA, COMPLEMENTARY; FLUORESCENT DYES; OLIGONUCLEOTIDES; SPECTROMETRY, FLUORESCENCE; THROMBIN;

EID: 0037462083     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja028962o     Document Type: Article

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21. One possible explanation that we would like to offer is that the electronic environment of the 5′-end of adenosine and its phosphate analogues affects the binding affinity. More specifically, whereas the anti-ATP aptamer can tolerate one negative charge on the alpha-phosphate group of ATP and ADP, the two negative charges on the monophosphate group of AMP interferes the aptamer-target binding. Although interesting, this point is not pursued further as the focus of the current investigation is directed at the designs of structure-switching signaling aptamer.
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