A homogeneous quenching resonance energy transfer assay for the kinetic analysis of the GTPase nucleotide exchange reaction

Analytical and Bioanalytical Chemistry

Volumn 406, Issue 17, 2014, Pages 4147-4156

  Kopra, Kari ^a   Ligabue, Alessio ^b   Wang, Qi ^a   Syrjänpää, Markku ^a   Blaževitš, Olga ^b   Veltel, Stefan ^c   Van Adrichem, Arjan J ^d   Hänninen, Pekka ^a   Abankwa, Daniel ^b   Härmä, Harri ^a  

^a UNIVERSITY OF TURKU   (Finland)

^b TURKU CENTRE FOR BIOTECHNOLOGY   (Finland)

^c UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

^d UNIVERSITY OF HELSINKI   (Finland)

Author keywords

Guanine nucleotide exchange factors; Quenching resonance energy transfer; Small GTPases; Time resolved luminescence

Indexed keywords

CATALYSIS; DISEASES; DISSOCIATION; ELECTROPHORESIS; ENERGY TRANSFER; EUROPIUM; KINETICS; NUCLEOTIDES; POSITIVE IONS; PROTEINS; QUENCHING; REACTION KINETICS; RESONANCE;

ASSOCIATION KINETICS; DISSOCIATION KINETICS; GTPASES; GUANINE NUCLEOTIDE EXCHANGE FACTORS; PROTEIN CONCENTRATIONS; RESONANCE ENERGY TRANSFER; SIGNAL-TO-BACKGROUND RATIO; TIME-RESOLVED LUMINESCENCE;

LOADING;

EUKARYOTA;

GUANOSINE TRIPHOSPHATASE; NUCLEOTIDE;

ANIMAL; BIOLUMINESCENCE RESONANCE ENERGY TRANSFER; CHEMISTRY; ENERGY TRANSFER; EVALUATION STUDY; HUMAN; PROCEDURES;

ANIMALS; BIOLUMINESCENCE RESONANCE ENERGY TRANSFER TECHNIQUES; ENERGY TRANSFER; GTP PHOSPHOHYDROLASES; HUMANS; NUCLEOTIDES;

EID: 84903776878     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-014-7795-7     Document Type: Article

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