메뉴 건너뛰기




Volumn 113, Issue 7, 2014, Pages 2671-2678

Quantification of viable Giardia cysts and Cryptosporidium oocysts in wastewater using propidium monoazide quantitative real-time PCR

Author keywords

Cryptosporidium; Giardia; PMA; qPCR; Viable; Wastewater

Indexed keywords

BETA GIARDIN; GLUTAMATE DEHYDROGENASE; PHOSPHATE BUFFERED SALINE; PROPIDIUM IODIDE; PROPIDIUM MONOAZIDE; PROTEIN; TRIOSEPHOSPHATE ISOMERASE; UNCLASSIFIED DRUG;

EID: 84903775611     PISSN: 09320113     EISSN: 14321955     Source Type: Journal    
DOI: 10.1007/s00436-014-3922-9     Document Type: Article
Times cited : (40)

References (27)
  • 1
    • 84890212606 scopus 로고    scopus 로고
    • Quantification of relative proportions of intact cells in microbiological samples using the example of Cryptosporidium parvum oocysts
    • Agulló-Barceló M, Moss JA, Green J, Gillespie S, Codony F, Lucena F, Nocker A (2014) Quantification of relative proportions of intact cells in microbiological samples using the example of Cryptosporidium parvum oocysts. Lett Appl Microbiol 58:70-78
    • (2014) Lett Appl Microbiol , vol.58 , pp. 70-78
    • Agulló-Barceló, M.1    Moss, J.A.2    Green, J.3    Gillespie, S.4    Codony, F.5    Lucena, F.6    Nocker, A.7
  • 2
    • 77956518398 scopus 로고    scopus 로고
    • Viability determination of Helicobacter pylori using propidium monoazide quantitative PCR
    • Agusti G, Codony F, Fittipaldi M, Adrados B, Morató J (2010) Viability determination of Helicobacter pylori using propidium monoazide quantitative PCR. Helicobacter 15:473-476
    • (2010) Helicobacter , vol.15 , pp. 473-476
    • Agusti, G.1    Codony, F.2    Fittipaldi, M.3    Adrados, B.4    Morató, J.5
  • 3
    • 77749303992 scopus 로고    scopus 로고
    • Genotyping of Giardia duodenalis cysts by new real-time PCR assays for detection of mixed infections in human samples
    • Almeida A, Pozio E, Cacciò SM (2010) Genotyping of Giardia duodenalis cysts by new real-time PCR assays for detection of mixed infections in human samples. Appl Environ Microbiol 76:1895-1901
    • (2010) Appl Environ Microbiol , vol.76 , pp. 1895-1901
    • Almeida, A.1    Pozio, E.2    Cacciò, S.M.3
  • 4
    • 79952534928 scopus 로고    scopus 로고
    • Development and evaluation of a real-time PCR assay for quantification of Giardia and Cryptosporidium in sewage samples
    • Alonso JL, Amorós I, Cañigral I (2011) Development and evaluation of a real-time PCR assay for quantification of Giardia and Cryptosporidium in sewage samples. Appl Microbiol Biotechnol 89:1203-1211
    • (2011) Appl Microbiol Biotechnol , vol.89 , pp. 1203-1211
    • Alonso, J.L.1    Amorós, I.2    Cañigral, I.3
  • 5
    • 81355154310 scopus 로고    scopus 로고
    • Waterborne transmission of protozoan parasites: A review of worldwide outbreaks - an update 2004-2010
    • Baldursson S, Karanis P (2011) Waterborne transmission of protozoan parasites: a review of worldwide outbreaks - an update 2004-2010. Water Res 45:6603-6614
    • (2011) Water Res , vol.45 , pp. 6603-6614
    • Baldursson, S.1    Karanis, P.2
  • 6
    • 79955456565 scopus 로고    scopus 로고
    • A real-time RT-PCR method to detect viable Giardia lamblia cysts in environmental waters
    • Baque RH, Gilliam AO, Robles LD, Jakubowski W, Slifko TR (2011) A real-time RT-PCR method to detect viable Giardia lamblia cysts in environmental waters. Water Res 45:3175-3184
    • (2011) Water Res , vol.45 , pp. 3175-3184
    • Baque, R.H.1    Gilliam, A.O.2    Robles, L.D.3    Jakubowski, W.4    Slifko, T.R.5
  • 8
    • 70350503115 scopus 로고    scopus 로고
    • Cryptosporidium propidium monoazide-PCR, a molecular biology-based technique for genotyping of viable Cryptosporidium oocysts
    • Brescia CC, Griffin SM, Ware MW, Varughese EA, Egorov AI, Villegas EN (2009) Cryptosporidium propidium monoazide-PCR, a molecular biology-based technique for genotyping of viable Cryptosporidium oocysts. Appl Environ Microbiol 75:6856-6863
    • (2009) Appl Environ Microbiol , vol.75 , pp. 6856-6863
    • Brescia, C.C.1    Griffin, S.M.2    Ware, M.W.3    Varughese, E.A.4    Egorov, A.I.5    Villegas, E.N.6
  • 10
    • 80052362316 scopus 로고    scopus 로고
    • Effect of PCR amplicon length on suppressing signals from membrane-compromised cells by propidium monoazide treatment
    • Contreras PJ, Urrutia H, Sossa K, Nocker A (2011) Effect of PCR amplicon length on suppressing signals from membrane-compromised cells by propidium monoazide treatment. J Microbiol Methods 87:89-95
    • (2011) J Microbiol Methods , vol.87 , pp. 89-95
    • Contreras, P.J.1    Urrutia, H.2    Sossa, K.3    Nocker, A.4
  • 12
    • 79960449862 scopus 로고    scopus 로고
    • Discrimination of viable Acanthamoeba castellani trophozoites and cysts by propidium monoazide real-time polymerase chain reaction
    • Fittipaldi M, Rodriguez NJ, Adrados B, Agustí G, Peñuela G, Morató J, Codony F (2011) Discrimination of viable Acanthamoeba castellani trophozoites and cysts by propidium monoazide real-time polymerase chain reaction. J Eukaryot Microbiol 58:359-364
    • (2011) J Eukaryot Microbiol , vol.58 , pp. 359-364
    • Fittipaldi, M.1    Rodriguez, N.J.2    Adrados, B.3    Agustí, G.4    Peñuela, G.5    Morató, J.6    Codony, F.7
  • 13
    • 84867754639 scopus 로고    scopus 로고
    • Progress in understanding preferential detection of live cells using viability dyes in combination with DNA amplification
    • Fittipaldi M, Nocker A, Codony F (2012) Progress in understanding preferential detection of live cells using viability dyes in combination with DNA amplification. J Microbiol Methods 91:276-289
    • (2012) J Microbiol Methods , vol.91 , pp. 276-289
    • Fittipaldi, M.1    Nocker, A.2    Codony, F.3
  • 14
    • 0141482157 scopus 로고    scopus 로고
    • Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage
    • DOI 10.1128/AEM.69.9.5178-5185.2003
    • Guy RA, Payment P, Krull UJ, Horgen PA (2003) Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage. Appl Environ Microbiol 69:5178-5185 (Pubitemid 37150736)
    • (2003) Applied and Environmental Microbiology , vol.69 , Issue.9 , pp. 5178-5185
    • Guy, R.A.1    Payment, P.2    Krull, U.J.3    Horgen, P.A.4
  • 15
    • 33745086594 scopus 로고    scopus 로고
    • A rapid molecular-based assay for direct quantification of viable bacteria in slaughterhouses
    • Guy RA, Kapoor A, Holicka J, Shepherd D, Horgen PA (2006) A rapid molecular-based assay for direct quantification of viable bacteria in slaughterhouses. J Food Protect 69:1265-1272 (Pubitemid 43887064)
    • (2006) Journal of Food Protection , vol.69 , Issue.6 , pp. 1265-1272
    • Guy, R.A.1    Kapoor, A.2    Holicka, J.3    Shepherd, D.4    Horgen, P.A.5
  • 16
    • 84867143685 scopus 로고    scopus 로고
    • Comparison of propidium monoazide-quantitative PCR and reverse transcription quantitative PCR for viability detection of fresh Cryptosporidium oocysts following disinfection and after long-term storage in water samples
    • Liang Z, Keeley A (2012) Comparison of propidium monoazide-quantitative PCR and reverse transcription quantitative PCR for viability detection of fresh Cryptosporidium oocysts following disinfection and after long-term storage in water samples. Water Res 46:5941-5953
    • (2012) Water Res , vol.46 , pp. 5941-5953
    • Liang, Z.1    Keeley, A.2
  • 17
    • 80052325467 scopus 로고    scopus 로고
    • Molecular identification and distribution of Cryptosporidium and Giardia duodenalis in raw urban wastewater in Harbin, China
    • Liu A, Ji H, Wang E, Liu J, Xiao L, Shen Y, Li Y, Zhang W, Ling H (2011) Molecular identification and distribution of Cryptosporidium and Giardia duodenalis in raw urban wastewater in Harbin, China. Parasitol Res 109:913-918
    • (2011) Parasitol Res , vol.109 , pp. 913-918
    • Liu, A.1    Ji, H.2    Wang, E.3    Liu, J.4    Xiao, L.5    Shen, Y.6    Li, Y.7    Zhang, W.8    Ling, H.9
  • 18
    • 58449118979 scopus 로고    scopus 로고
    • Novel approaches toward preferential detection of viable cells using nucleic acid amplification techniques
    • Nocker A, Camper AK (2009) Novel approaches toward preferential detection of viable cells using nucleic acid amplification techniques. FEMS Microbiol Lett 291:137-142
    • (2009) FEMS Microbiol Lett , vol.291 , pp. 137-142
    • Nocker, A.1    Camper, A.K.2
  • 19
    • 33748746842 scopus 로고    scopus 로고
    • Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells
    • DOI 10.1016/j.mimet.2006.04.015, PII S0167701206001254
    • Nocker A, Cheung C-Y, Camper AK (2006) Comparison of propidium monoazide with ethidium monoazide for determination of live vs. dead bacteria by selective removal of DNA from dead cells. J Microbiol Methods 67:310-320 (Pubitemid 44402748)
    • (2006) Journal of Microbiological Methods , vol.67 , Issue.2 , pp. 310-320
    • Nocker, A.1    Cheung, C.-Y.2    Camper, A.K.3
  • 20
    • 74249111651 scopus 로고    scopus 로고
    • A study of PCR inhibition mechanisms using real time PCR
    • Opel KL, Chun D, McCord BR (2010) A study of PCR inhibition mechanisms using real time PCR. J Forensic Sci 55:25-33
    • (2010) J Forensic Sci , vol.55 , pp. 25-33
    • Opel, K.L.1    Chun, D.2    McCord, B.R.3
  • 21
    • 38449083968 scopus 로고    scopus 로고
    • Risk of waterborne illness via drinking water in the United States
    • Reynolds KA, Mena KD, Gerba CP (2008) Risk of waterborne illness via drinking water in the United States. Rev Environ Contam Toxicol 192:117-158
    • (2008) Rev Environ Contam Toxicol , vol.192 , pp. 117-158
    • Reynolds, K.A.1    Mena, K.D.2    Gerba, C.P.3
  • 22
    • 84888312262 scopus 로고    scopus 로고
    • Drying of Cryptosporidium oocysts and Giardia cysts to slides abrogates use of vital dyes for viability staining
    • Robertson LJ, Casaert S, Valdez-Nava Y, Ehsan MA, Claerebout E (2014) Drying of Cryptosporidium oocysts and Giardia cysts to slides abrogates use of vital dyes for viability staining. J Microbiol Methods 96:68-69
    • (2014) J Microbiol Methods , vol.96 , pp. 68-69
    • Robertson, L.J.1    Casaert, S.2    Valdez-Nava, Y.3    Ehsan, M.A.4    Claerebout, E.5
  • 23
    • 0024835357 scopus 로고
    • A comparison of fluorescein diacetate and propidium iodide staining and in vitro excystation for determining Giardia intestinalis cyst viability
    • Smith AL, Smith HV (1989) A comparison of fluorescein diacetate and propidium iodide staining and in vitro excystation for determining Giardia intestinalis cyst viability. Parasitology 99:329-331 (Pubitemid 20064087)
    • (1989) Parasitology , vol.99 , Issue.3 , pp. 329-331
    • Smith, A.L.1    Smith, H.V.2
  • 24
    • 34548614605 scopus 로고    scopus 로고
    • Cryptosporidium and Giardia as foodborne zoonoses
    • DOI 10.1016/j.vetpar.2007.07.015, PII S0304401707003536, Food-Borne Parasitic Zoonosis Papers presented at the Fourth Annual Meeting of the European Veterinary Parasitology College (EVPC)
    • Smith HV, Cacciò SM, Cook N, Nichols RAB, Tait A (2007) Cryptosporidium and Giardia as foodborne zoonoses. Vet Parasitol 149:29-40 (Pubitemid 47398932)
    • (2007) Veterinary Parasitology , vol.149 , Issue.1-2 , pp. 29-40
    • Smith, H.V.1    Caccio, S.M.2    Cook, N.3    Nichols, R.A.B.4    Tait, A.5
  • 26
    • 2942595718 scopus 로고    scopus 로고
    • Distribution of Giardia duodenalis genotypes and subgenotypes in raw urban wastewater in Milwaukee, Wisconsin
    • DOI 10.1128/AEM.70.6.3776-3780.2004
    • Sulaiman IM, Jiang J, Singh A, Xiao L (2004) Distribution of Giardia duodenalis genotypes and subgenotypes in raw urban wastewater in Milwaukee, Wisconsin. Appl Environ Microbiol 70:3776-3780 (Pubitemid 38745935)
    • (2004) Applied and Environmental Microbiology , vol.70 , Issue.6 , pp. 3776-3780
    • Sulaiman, I.M.1    Jiang, J.2    Singh, A.3    Xiao, L.4
  • 27
    • 38049016343 scopus 로고    scopus 로고
    • Quantifying fungal viability in air and water samples using quantitative PCR after treatment with propidium monoazide (PMA)
    • Vesper S, McKinstry C, Hartmann C, Neace M, Yoder S, Vesper A (2008) Quantifying fungal viability in air and water samples using quantitative PCR after treatment with propidium monoazide (PMA). J Microbiol Methods 72:180-184
    • (2008) J Microbiol Methods , vol.72 , pp. 180-184
    • Vesper, S.1    McKinstry, C.2    Hartmann, C.3    Neace, M.4    Yoder, S.5    Vesper, A.6


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.