Comparison of propidium monoazide-quantitative PCR and reverse transcription quantitative PCR for viability detection of fresh Cryptosporidium oocysts following disinfection and after long-term storage in water samples

Water Research

Volumn 46, Issue 18, 2012, Pages 5941-5953

  Liang, Zhanbei ^a   Keeley, Ann ^b  

^a National Research Council   (United States)

^b U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

Author keywords

Cryptosporidium parvum; Disinfection; Long term storage; MRNA; PMA; Water concentrate

Indexed keywords

AMMONIA; DISINFECTION; DNA; HYDROGEN PEROXIDE; OXIDATION; PEROXIDES; SURFACE WATERS; TRANSCRIPTION; WATER TREATMENT;

CRYPTOSPORIDIUM OOCYSTS; CRYPTOSPORIDIUM PARVUM; HYDROGEN PEROXIDE TREATMENT; LONG-TERM STORAGE; MRNA; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION QUANTITATIVE-PCR; VIABILITY EVALUATION;

POLYMERASE CHAIN REACTION;

AMMONIA; GENOMIC DNA; HEAT SHOCK PROTEIN 70; HYDROGEN PEROXIDE; MESSENGER RNA; PROPIDIUM MONOAZIDE; REAGENT; SURFACE WATER; UNCLASSIFIED DRUG;

DETECTION METHOD; DISINFECTION; FLOW CYTOMETRY; HYDROGEN PEROXIDE; MEMBRANE; POLYMERASE CHAIN REACTION; PROTOZOAN; QUANTITATIVE ANALYSIS; SURFACE WATER; SUSPENDED LOAD;

ARTICLE; CELL VIABILITY; COMPARATIVE STUDY; CRYPTOSPORIDIUM PARVUM; DISINFECTION; DNA EXTRACTION; FLOW CYTOMETRY; GENE TARGETING; NONHUMAN; OOCYST; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STORAGE; WATER SAMPLING; WATER STANDARD; WATER TREATMENT;

CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM;

EID: 84867143685     PISSN: 00431354     EISSN: 18792448     Source Type: Journal    
DOI: 10.1016/j.watres.2012.08.014     Document Type: Article

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