Multicolor staining of globin subtypes reveals impaired globin switching during erythropoiesis in human pluripotent stem cells

Stem Cells Translational Medicine

Volumn 3, Issue 7, 2014, Pages 792-800

  Ochi, Kiyosumi ^a,b   Takayama, Naoya ^a   Hirose, Shoichi ^b,c   Nakahata, Tatsutoshi ^a   Nakauchi, Hiromitsu ^b   Eto, Koji ^a  

^a KYOTO UNIVERSITY   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

^c TERUMO CORPORATION   (Japan)

Author keywords

BCL11A; Embryonic stem cells; Erythrocyte; Globin switching; IPS cells

Indexed keywords

CD34 ANTIBODY; DOXYCYCLINE; GLOBIN; GREEN FLUORESCENT PROTEIN; HEMOGLOBIN; HEMOGLOBIN ALPHA CHAIN; HEMOGLOBIN BETA CHAIN; HEMOGLOBIN GAMMA CHAIN; LENTIVIRUS VECTOR; MEMBRANE ANTIGEN; MITOMYCIN; PROTEIN C MYB; TRANSCRIPTION FACTOR GATA 1; TRANSCRIPTION FACTOR SOX6;

ARTICLE; BCL11A GENE; CELL DIFFERENTIATION; CELL EXPANSION; DOWN REGULATION; ERYTHROPOIESIS; FLOW CYTOMETRY; GENE; GENE SILENCING; GENETIC TRANSFECTION; HEMATOPOIETIC STEM CELL; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; IMAGE ANALYSIS; IMMUNOFLUORESCENCE; PLURIPOTENT STEM CELL; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; UPREGULATION;

BCL11A; EMBRYONIC STEM CELLS; ERYTHROCYTE; GLOBIN SWITCHING; IPS CELLS;

ANTIGENS, CD34; BETA-GLOBINS; BIOLOGICAL MARKERS; CARRIER PROTEINS; CELL DIFFERENTIATION; COCULTURE TECHNIQUES; ERYTHROPOIESIS; FEEDER CELLS; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; GAMMA-GLOBINS; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENE SILENCING; HEMATOPOIETIC STEM CELLS; HEMOGLOBIN SUBUNITS; HUMANS; MOLECULAR PROBES; NUCLEAR PROTEINS; PLURIPOTENT STEM CELLS; TIME FACTORS; TRANSFECTION;

EID: 84903511243     PISSN: 21576564     EISSN: 21576580     Source Type: Journal    
DOI: 10.5966/sctm.2013-0216     Document Type: Article

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