The neuroprotective efficacy of cell-penetrating peptides TAT, penetratin, Arg-9, and Pep-1 in glutamic acid, kainic acid, and in vitro ischemia injury models using primary cortical neuronal cultures

Cellular and Molecular Neurobiology

Volumn 34, Issue 2, 2014, Pages 173-181

  Meloni, Bruno P ^a,b   Craig, Amanda J ^a   Milech, Nadia ^c   Hopkins, Richard M ^c   Watt, Paul M ^c   Knuckey, Neville W ^a,b  

^a UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

^b SIR CHARLES GAIRDNER HOSPITAL   (Australia)

^c UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

Author keywords

Arg 9; Cell penetrating peptides; Excitotoxicity; In vitro ischemia; Penetratin; Pep 1; TAT

Indexed keywords

CELL PENETRATING PEPTIDE; CELL PENETRATING PEPTIDE ARG 9; CELL PENETRATING PEPTIDE PEP 1; GLUTAMIC ACID; KAINIC ACID; NEUROPROTECTIVE AGENT; PENETRATIN; TRANSACTIVATOR PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN CELL CULTURE; BRAIN ISCHEMIA; CONTROLLED STUDY; DRUG EFFICACY; IN VITRO STUDY; NEUROPROTECTION; NONHUMAN; PRIMARY CULTURE; PRIORITY JOURNAL; RAT;

AMINO ACID SEQUENCE; ANIMALS; CARRIER PROTEINS; CELL SURVIVAL; CELL-PENETRATING PEPTIDES; CELLS, CULTURED; CEREBRAL CORTEX; CYSTEAMINE; GENE PRODUCTS, TAT; GLUTAMIC ACID; INHIBITORY CONCENTRATION 50; ISCHEMIA; KAINIC ACID; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; NEURONS; NEUROPROTECTIVE AGENTS; NEUROTOXINS; OLIGOPEPTIDES; PEPTIDES; RATS; RATS, SPRAGUE-DAWLEY;

EID: 84903371710     PISSN: 02724340     EISSN: 15736830     Source Type: Journal    
DOI: 10.1007/s10571-013-9999-3     Document Type: Article

References (33)

1. Aarts M, Liu Y, Liu L, Besshoh S, Arundine M, Gurd JW, Wang YT, Salter MW, Tymianski M (2002) Treatment of ischemic brain damage by perturbing NMDA receptor-PSD-95 protein interactions. Science 298:846-850 (Pubitemid 35231541)
2. ARAMIS (2011) Project number: 8909.1. http://www.aramis.admin. ch. Accessed 2 Nov 2013
3. Arthur PG, Matich GP, Pang WW, Yu DY, Bogoyevitch MA (2007) Necrotic death of neurons following an excitotoxic insult is prevented by a peptide inhibitor of c-jun N-terminal kinase. J Neurochem 102:65-76 (Pubitemid 46906274)
4. Ashpole NM, Hudmon A (2011) Excitotoxic neuroprotection and vulnerability with CaMKII inhibition. Mol Cell Neurosci 46:720-730
5. Borsello T, Clarke PG, Hirt L, Vercelli A, Repici M, Schorderet DF, Bogousslavsky J, Bonny C (2003) A peptide inhibitor of c-Jun N-terminal kinase protects against excitotoxicity and cerebral ischemia. Nat Med 9:1180-1186 (Pubitemid 37173703)
6. Cardozo AK, Buchillier V, Mathieu M, Chen J, Ortis F, Ladrière L, Allaman-Pillet N, Poirot O, Kellenberger S, Beckmann JS, Eizirik DL, Bonny C, Maurer F (2007) Cell-permeable peptides induce dose-and length-dependent cytotoxic effects. Biochim Biophys Acta 1768:2222-2234 (Pubitemid 47379621)
7. Colombo A, Repici M, Pesaresi M, Santambrogio S, Forloni G, Borsello T (2007) The TAT-JNK inhibitor peptide interferes with beta amyloid protein stability. Cell Death Differ 14:1845-1848 (Pubitemid 47423652)
8. Craig AJ, Meloni BP, Watt PM, Knuckey NW (2011) Attenuation of neuronal death by peptide inhibitors of AP-1 activation in acute and delayed in vitro ischaemia (oxygen/glucose deprivation) models. Int J Pept Res Ther 17:1-6
9. Doeppner TR, Nagel F, Dietz GPH, Weise J, Tönges L, Schwarting S, Bähr M (2009) TAT-HSP70-mediated neuroprotection and increased survival of neuronal precursor cells after focal cerebral ischemia in mice. J Cereb Blood Flow Metab 29:1187-1196
10. Dolgin E (2012) To serve and neuroprotect. Nat Med 18:1003-1006
11. Ferrer-Montiel AV, Merino JM, Blondelle SE, Perez-Payà E, Houghten RA, Montal M (1998) Selected peptides targeted to the NMDA receptor channel protect neurons from excitotoxic death. Nat Biotechnol 16:286-291 (Pubitemid 28120110)
12. Frankel AD, Pabo CO (1988) Cellular uptake of the TAT protein from human immunodeficiency virus. Cell 55:1189-1193 (Pubitemid 19020071)
13. Green M, Loewenstein PM (1988) Autonomous functional domains of chemically synthesized human immunodeficiency virus TAT trans-activator protein. Cell 55:1179-1188 (Pubitemid 19020070)
14. Hirose H, Takeuchi T, Osakada H, Pujals S, Katayama S, Nakase I, Kobayashi S, Haraguchi T, Futaki S (2012) Transient focal membrane deformation induced by arginine-rich peptides leads to their direct penetration into cells. Mol Ther 20:984-993
15. Ho A, Schwarze SR, Mermelstein SJ, Waksman G, Dowdy SF (2001) Synthetic protein transduction domains: enhanced transduction potential in vitro and in vivo. Cancer Res 61:474-477 (Pubitemid 32128600)
16. Kacprzak MM, Peinado JR, Than ME, Appel J, Henrich S, Lipkind G, Houghten RA, Bode W, Lindberg I (2004) Inhibition of furin by polyarginine-containing peptides: nanomolar inhibition by nona-D-arginine. J Biol Chem 279:36788-36794 (Pubitemid 39129027)
17. Kilic Ü, Kilic E, Dietz GPH, Bähr M (2003) Intravenous TAT-GDNF is protective after focal cerebral ischemia in mice. Stroke 34:1304-1310
18. Lai Y, Du L, Dunsmore KE, Jenkins LW, Wong HR, Clark RS (2005) Selectively increasing inducible heat shock protein 70 via TATprotein transduction protects neurons from nitrosative stress and excitotoxicity. J Neurochem 94:360-366 (Pubitemid 40995252)
19. Li W, Huang Y, Reid R, Steiner J, Malpica-Llanos T, Darden TA, Shankar SK, Mahadevan A, Satishchandra P, Nath A (2008) NMDA receptor activation by HIV-Tat protein is clade dependent. J Neurosci 28:12190-12198
20. Liu XM, Pei DS, Guan QH, Sun YF, Wang XT, Zhang QX, Zhang GY (2006) Neuroprotection of TAT-GluR6-9c against neuronal death induced by kainate in rat hippocampus via nuclear and non-nuclear pathways. J Biol Chem 281:17432-17445
21. Meade AJ, Meloni BP, Mastaglia FL, Knuckey NW (2009) The application of cell penetrating peptides for the delivery of neuroprotective peptides/proteins in experimental cerebral ischemia studies. J Exp Stroke Transl Med 2:22-40
22. Meade AJ, Meloni BP, Mastaglia FL, Watt PM, Knuckey NW (2010a) AP-1 inhibitory peptides attenuate in vitro cortical neuronal cell death induced by kainic acid. Brain Res 1360:8-16
23. Meade AJ, Meloni BP, Cross J, Bakker AJ, Fear MW, Mastaglia FL, Watt PM, Knuckey NW (2010b) AP-1 inhibitory peptides are neuroprotective following acute glutamate excitotoxicity in primary cortical neuronal cultures. J Neurochem 112:258-270
24. Meloni BP, Majda BT, Knuckey NW (2001) Establishment of neuronal in vitro models of ischemia in 96-well microtiter strip-plates that result in acute, progressive and delayed neuronal death. Neuroscience 108:17-26 (Pubitemid 33152978)
25. Meloni BP, Meade AJ, Kitikomolsuk D, Knuckey NW (2011) Characterisation of neuronal cell death in acute and delayed in vitro ischemia (oxygen/glucose deprivation) models. J Neurosci Methods 195:67-74
26. Milletti F (2012) Cell-penetrating peptides: classes, origin, and current landscape. Drug Discov Today 17:850-860
27. Moschos SA, Jones SW, Perry MM, Williams AE, Erjefalt JS, Turner JJ, Barnes PJ, Sproat BS, Gait MJ, Lindsay MA (2007) Lung delivery studies using siRNA conjugated to TAT(48-60) and penetratin reveal peptide induced reduction in gene expression and induction of innate immunity. Bioconjug Chem 18:1450-1459 (Pubitemid 47464052)
28. Nagel F, Falkenburger BH, Tönges L, Kowsky S, Pöppelmeyer C, Schulz JB, Bähr M, Dietz GP (2008) TAT-HSP70 protects dopaminergic neurons in midbrain cultures and in the substantia nigra in models of Parkinson's disease. J Neurochem 105:853-864 (Pubitemid 351562740)
29. Nath A, Psooy K, Martin C, Knudsen B, Magnuson DS, Haughey N, Geiger JD (1996) Identification of a human immunodeficiency virus type 1 TAT epitope that is neuroexcitatory and neurotoxic. J Virol 70:1475-1480 (Pubitemid 26062905)
30. Palm-Apergi C, Lönn P, Dowdy SF (2012) Do cell-penetrating peptides actually "penetrate" cellular membranes? Mol Ther 20:695-697
31. Vaslin A, Rummel C, Clarke PG (2009) Unconjugated TAT carrier peptide protects against excitotoxicity. Neurotox Res 15:123-126
32. Xu W, Zhou M, Baudry M (2008) Neuroprotection by cell permeable TAT-mGluR1 peptide in ischemia: synergy between carrier and cargo sequences. Neuroscientist 14:409-414
33. Zhang S, Taghibiglou C, Girling K, Dong Z, Lin SZ, Lee W, Shyu WC, Wang YT (2013) Critical role of increased PTEN nuclear translocation in excitotoxic and ischemic neuronal injuries. J Neurosci 33:7997-8008