Engineered Cyanobacteria: Research and Application in Bioenergy

Bioenergy Research: Advances and Applications

Volumn , Issue , 2014, Pages 389-406

  Leite, Gustavo B ^a   Hallenbeck, Patrick C ^a  

^a UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

Biofuel; Carbon dioxide; Cyanobacteria; Photosynthesis

Indexed keywords

BIOREMEDIATION; CARBON DIOXIDE; CHEMICAL COMPOUNDS; CHEMICAL INDUSTRY; CRUDE OIL; EFFLUENTS; FUELS; GEOTHERMAL ENERGY; HEATING; METABOLIC ENGINEERING; MOLECULES; OILS AND FATS; PHOTOSYNTHESIS; WASTEWATER TREATMENT;

CYANOBACTERIA; ENERGY PRODUCTIONS; GENETIC MANIPULATIONS; NATURAL PRODUCTIONS; PRODUCTION SYSTEM; RESEARCH AND APPLICATION; SUSTAINABLE ENERGY TECHNOLOGY; WASTEWATER TREATMENT PLANTS;

BIOFUELS;

EID: 84903103720     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1016/B978-0-444-59561-4.00022-X     Document Type: Chapter

References (119)

1. Abeles A.L. Biochemical pathway of stress-induced ethylene. Plant Physiol. 1972, 50:496-498.
2. Ananyev G.M., Skizim N.J., Dismukes G.C. Enhancing biological hydrogen production from cyanobacteria by removal of excreted products. J. Biotechnol. 2012, 162:97-104.
3. Angermayr S.A., Hellingwerf K.J., Lindblad P., Teixeira de Mattos M.J. Energy biotechnology with cyanobacteria. Curr. Opin. Biotechnol. 2009, 20:257-263. NCBI:. http://www.ncbi.nlm.nih.gov.
4. 2 production. Appl. Environ. Microbiol. 2013, 79:1070-1077.
5. Atsumi S., Higashide W., Liao J.C. Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde. Nat. Biotechnol. 2009, 27:1177-1180.
6. Ball P. Burning Water and Other Myths 2007, http://news@nature/.
7. 2 production by a cyanobacterium under aerobic conditions. Nat. Commun. 2010, 1:139-147.
8. Benemann J.R., Weare N.M. Hydrogen evolution by nitrogen-fixing Anabaena cylindrical cultures. Science 1974, 184:174-175.
9. Berberoglu H. Effect of nutrient media on photobiological hydrogen production by Anabaena variabilis ATCC 29413. Int. J. Hydrogen Energy 2008, 33:1172-1184.
10. Berman-Frank I. Segregation of nitrogen fixation and oxygenic photosynthesis in the marine cyanobacterium Trichodesmium. Science 2001, 294:1534-1537.
11. Bothe H., Schmitz O., Yates M.G., Newton W.E. Nitrogen fixation and hydrogen metabolism in cyanobacteria. Microbiol. Mol. Biol. Rev. 2010, 74:529-551.
12. BP, 2012. BP Statistical Review of World Energy June 2012. BP. p. 48. http://bp.com/statisticalreview.
13. Das D., Eser B.E., Han J., Sciore A., Marsh E.N.G. Oxygen-Independent Decarbonylation of Aldehydes by Cyanobacterial Aldehyde Decarbonylase: A New Reaction of Diiron Enzymes. Angewandte Chemie International Edition 2011, 50(31):7148-7152.
14. Deng M.D., Coleman J.R. Ethanol synthesis by genetic engineering in cyanobacteria. Appl. Environ. Microbiol. 1999, 65:523-528.
15. Dexter J., Fu P. Metabolic engineering of cyanobacteria for ethanol production. Energy Environ. Sci. 2009, 2:857.
16. Ducat D.C., Way J.C., Silver P.A. Engineering cyanobacteria to generate high-value products. Trends. Biotechnol. 2011, 29:95-103.
17. Dutheil J., Saenkham P., Sakr S., Leplat C., Ortega-Ramos M., Bottin H., Cournac L., Cassier-Chauvat C., Chauvat F. The AbrB2 autorepressor, expressed from an atypical promoter, represses the hydrogenase operon to regulate hydrogen production in Synechocystis strain PCC6803. J. Bacteriol. 2012, 194:5423-5433.
18. EIADOE Primary energy consumption by source and sector, 2011 384(null). EIA - US Energy Information Administration 2012, vol. 1. http://www.eia.gov/aer.
19. Elhai J., Vepritskiy A., MuroPastor A.M., Flores E., Wolk C.P. Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120. J. Bacteriol. 1997, 179:1998-2005.
20. Field C.B., Behrenfeld M.J., Randerson J.T., Falkowski P. Primary production of the biosphere: integrating terrestrial and oceanic components. Science 1998, 281:237-240.
21. Flores E., Muro-Pastor A.M., Meeks J.C. Gene transfer to cyanobacteria in the laboratory and in nature. The Cyanobacteria: Molecular Biology, Genomics and Evolution 2008, Caister Academic Press. A. Herrero, E. Flores (Eds.).
22. Freebairn H.T., Buddenhagen I.W. Ethylene production by Pseudomonas solanacearum. Nature 1964, 202:313-314.
23. Fukuda H., Ogawa T., Ishihara K., Fujii T., Nagahama K., Omata T., Inoue Y., Tanase S., Morino Y. Molecular-cloning in Escherichia coli, expression, and Nucleotide-sequence of the gene for the ethylene-forming enzyme of Pseudomonas syringae pv phaseolicola-Pk2. Biochem. Biophys. Res. Commun. 1992, 188:826-832.
24. Fukuda H., Ogawa T., Tazaki M., Nagahama K., Fujii T., Tanase S., Morino Y. Two reactions are simultaneously catalyzed by a single enzyme: the arginine-dependent simultaneous formation of two products, ethylene and succinate, from 2-oxoglutarate by an enzyme from Pseudomonas syringae. Biochem. Biophys. Res. Commun. 1992, 188:483-489.
25. Fukuda H., Sakai M., Nagahama K., Fujii T., Matsuoka M., Inoue Y., Ogawa T. Heterologous expression of the gene for the ethylene-forming enzyme from Pseudomonas syringae in the cyanobacterium Synechococcus. Biotechnol. Lett. 1994, 16:1-6.
26. Gao Z., Zhao H., Li Z., Tan X., Lu X. Photosynthetic production of ethanol from carbon dioxide in genetically engineered cyanobacteria. Energy Environ. Sci. 2012, 5:9857.
27. Gartner K., Lechno-Yossef S., Cornish A.J., Wolk C.P., Hegg E.L. Expression of Shewanella oneidensis MR-1 [FeFe]-Hydrogenase genes in Anabaena sp. Strain PCC 7120. Appl. Environ. Microbiol. 2012, 78:8579-8586.
28. Ghirardi M.L., Posewitz M.C., Maness P.-C., Dubini A., Yu J., Seibert M. Hydrogenases and hydrogen photoproduction in oxygenic photosynthetic organisms. Annu. Rev. Plant Biol. 2007, 58:71-91.
29. Golden S.S., Sherman L.A. A hybrid plasmid is a stable cloning vector for the cyanobacterium Anacystis nidulans R2. J. Bacteriol. 1983, 155:966-972.
30. Grigorieva G., Shestakov S. Transformation in the cyanobacterium Synechocystis sp. 6803. FEMS. Microbiol. Lett. 1982, 13:367-370.
31. Guiry, M.D., Guiry, G.M. Algaebase. National University of Ireland, Galway. http://www.algaebase.org.
32. Hallenbeck P.C., Benemann J.R. Characterization and partial purification of reversible hydrogenase of Anabaena cylindrica. FEBS Lett. 1978, 94:261-264.
33. Hallenbeck P.C., Benemann J.R. Biological hydrogen production; fundamentals and limiting processes. Int. J. Hydrogen. Energy. 2002, 27:1185-1193.
34. Hallenbeck P.C. Hydrogen production by cyanobacteria. Microbial Technologies in Advanced Biofuels Production 2011, 15-28. Springer US, Boston, MA. P.C. Hallenbeck (Ed.).
35. Han J., McCarthy E.D., Hoeven W.V., Calvin M., Bradley W.H. Organic geochemical studies, II. a preliminary report on the distribution of aliphatic hydrocarbons in algae, in bacteria, and in a recent lake sediment. Proc. Natl. Acad. Sci. USA. 1968, 59:29-33.
36. Hasegawa M., Nishizawa A., Tsuji K., Kimura S., Harada K.-I. Volatile organic compounds derived from 2-Keto-Acid decarboxylase in Microcystis aeruginosa. Microb. Environ. 2012, 27:525-528.
37. Heidorn T., Camsund D., Huang H.-H., Lindberg P., Oliveira P., Stensjö K., Lindblad P. Synthetic biology in cyanobacteria: engineering and analyzing novel functions. Synthetic Biology, Methods for Part/Device Characterization and Chassis Engineering, Part A 2011, vol. 497:539-579. Elsevier academic press inc, 525 B Street, suite 1900, San Diego, CA 92101-4495, USA. C. Voigt (Ed.).
38. Huesemann M.H., Hausmann T.S., Carter B.M., Gerschler J.J., Benemann J.R. Hydrogen generation through indirect biophotolysis in batch cultures of the nonheterocystous nitrogen-fixing cyanobacterium Plectonema boryanum. Appl. Biochem. Biotechnol. 2009, 162:208-220.
39. Ishihara K., Matsuoka M., Inoue Y., Tanase S., Ogawa T., Fukuda H. Overexpression and in-vitro reconstitution of the ethylene-forming enzyme from Pseudomonas syringae. J. Ferment. Bioeng. 1995, 79:205-211.
40. Ishihara K., Matsuoka M., Ogawa T., Fukuda H. Ethylene production using a broad-host-range plasmid in Pseudomonas syringae and Pseudomonas putida. J. Ferment. Bioeng. 1996, 82:509-511.
41. Izumikawa M., Khan S.T., Takagi M., Shin-ya K. Sponge-derived Streptomyces producing isoprenoids via the mevalonate pathway. J. Nat. Prod. 2010, 73:208-212.
42. Julsing M.K., Rijpkema M., Woerdenbag H.J., Quax W.J., Kayser O. Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis. Appl. Microbiol. Biotechnol. 2007, 75:1377-1384.
43. Kilian O., Benemann C.S.E., Niyogi K.K., Vick B. High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp. Proc. Natl. Acad. Sci. USA. 2011, 108:21265-21269.
44. Knoll A. Cyanobacteria and earth history. The Cyanobacteria: Molecular Biology, Genomics and Evolution 2008, Caister Academic Press. A. Herrero, E. Flores (Eds.).
45. Koksharova O.A., Wolk C.P. Genetic tools for cyanobacteria. Appl. Microbiol. Biotechnol. 2002, 58:123-137.
46. Kothari A., Potrafka R., Garcia-Pichel F. Diversity in hydrogen evolution from bidirectional hydrogenases in cyanobacteria from terrestrial, freshwater and marine intertidal environments. J. Biotechnol. 2012, 162:105-114.
47. Kumar K., Mella-Herrera R.A., Golden J.W. Cyanobacterial heterocysts. Cold Spring Harbor Perspect. Biol. 2010, 2:a000315.
48. Kuzma J., Nemecek-Marshall M., Pollock W.H., Fall R. Bacteria produce the volatile hydrocarbon isoprene. Curr. Microbiol. 1995, 30:97-103.
49. Lan E.I., Liao J.C. Metabolic engineering of cyanobacteria for 1-butanol production from carbon dioxide. Metab. Eng. 2011, 13:353-363.
50. Leplat C., Champeimont R., Saenkham P., Cassier-Chauvat C., Jean-Christophe A., Chauvat F. Genome-wide transcriptome analysis of hydrogen production in the cyanobacterium Synechocystis: towards the identification of new players. Int. J. Hydrogen Energy 2013, 38:1866-1872.
51. Lindberg P., Park S., Melis A. Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism. Metab. Eng. 2010, 12:70-79.
52. Lindblad P., Lindberg P., Oliveira P., Stensjö K., Heidorn T. Design, engineering, and construction of photosynthetic microbial cell factories for renewable solar fuel production. Ambio 2012, 41:163-168.
53. Lopez-Igual R., Flores E., Herrero A. Inactivation of a heterocyst-specific invertase indicates a principal role of sucrose catabolism in heterocysts of Anabaena sp. J. Bacteriol. 2010, 192:5526-5533.
54. Ludwig M., Schulz-Friedrich R., Appel J. Occurrence of hydrogenases in cyanobacteria and anoxygenic photosynthetic bacteria: Implications for the phylogenetic origin of cyanobacterial and algal hydrogenases. J. Mol. Evol. 2006, 63:758-768.
55. Mariscal V., Flores E. Multicellularity in a Heterocyst-forming Cyanobacterium: Pathways for Intercellular Communication 2010, vol. 675:123-135. Springer-Verlag Berlin, Heidelberger Platz 3, D-14197, Berlin, Germany.
56. Marreiros B.C., Batista A.P., Duarte A.M.S., Pereira M.M. A missing link between complex I and group 4 membrane-bound [NiFe] hydrogenases. Biochim. Biophys. 2013, 1827:198-209.
57. McCoy M., Reisch M., Tullo A.H., Short P.L., Tremblay J- F. Production: growth is the norm. Chem. Eng. News Arch. 2006, 84:59-68.
58. McNeely K., Xu Y., Bennette N., Bryant D.A., Dismukes G.C. Redirecting reductant flux into hydrogen production via metabolic engineering of fermentative carbon metabolism in a cyanobacterium. Appl. Environ. Microbiol. 2010, 76:5032-5038.
59. 2 production driven by photosynthetic water splitting in a unicellular cyanobacterium. mBio 2012, 3:e00197-e00212.
60. Miller B., Oschinski C., Zimmer W. First isolation of an isoprene synthase gene from poplar and successful expression of the gene in Escherichia coli. Planta 2001, 213:483-487.
61. Min H., Sherman L.A. Hydrogen production by the unicellular, diazotrophic cyanobacterium Cyanothece sp. strain ATCC 51142 under conditions of continuous light. Appl. Environ. Microbiol. 2010, 76:4293-4301.
62. Miyamoto K., Hallenbeck P.C., Benemann J.R. Effects of nitrogen supply on hydrogen production by cultures of Anabaena cylindrica. Biotechnol. Bioeng. 1979, 21:1855-1860.
63. Miyamoto K., Hallenbeck P.C., Benemann J.R. Hydrogen production by the thermophilic alga Mastigocladus laminosus - effects of nitrogen, temperature, and inhibition of photosynthesis. Appl. Environ. Microbiol. 1979, 38:440-446.
64. Miyamoto K., Hallenbeck P.C., Benemann J.R. Nitrogen-fixation by thermophilic blue-green algae (cyanobacteria) - temperature characteristics and potential use in biophotolysis. Appl. Environ. Microbiol. 1979, 37:454-458.
65. Miyamoto K., Hallenbeck P.C., Benemann J.R. Solar energy conversion by nitrogen limited cultures of Anabaena cylindrical. J. Ferment. Technol. 1979, 57:287-293.
66. Murry M.A., Hallenbeck P.C., Benemann J.R. Immunochemical evidence that nitrogenase is restricted to the heterocysts in Anabaena cylindrica. Arch. Microbiol. 1984, 137:194-199.
67. Murry M.A., Hallenbeck P.C., Esteva D., Benemann J.R. Nitrogenase inactivation by oxygen and enzyme turnover in Anabaena cylindrica. Can. J. Microbiol. 1983, 29:1286-1294.
68. Nagahama K., Ogawa T., Fujii T., Fukuda H. Classification of ethylene-producing bacteria in terms of biosynthetic pathways to ethylene. J. Ferment. Bioeng. 1992, 73:1-5.
69. Nagahama K., Ogawa T., Fujii T., Tazaki M., Tanase S., Morino Y., Fukuda H. Purification and properties of an ethylene-forming enzyme from Pseudomonas syringae pv. phaseolicola PK2. J. Gen. Microbiol. 1991, 137:2281-2286.
70. Nakatsuka A., Murachi S., Okunishi H., Shiomi S., Nakano R., Kubo Y., Inaba A. Differential expression and internal feedback regulation of 1-aminocyclopropane-1-carboxylate synthase, 1-aminocyclopropane-1-carboxylate oxidase, and ethylene receptor genes in tomato fruit during development and ripening. Plant. Physiol. 1998, 118:1295-1305.
71. Nashawi I.S., Malallah A., Al-Bisharah M. Forecasting world crude oil production using multicyclic Hubbert model. Energy Fuels 2010, 24:1788-1800.
72. Ogawa T., Takahashi M., Fujii T., Tazaki M., Fukuda H. The role of NADH: Fe(III)EDTA oxidoreductase in ethylene formation from 2-Keto-4-methylthiobutyrate. J. Ferment. Bioeng. 1990, 69:287-291.
73. Oliveira P., Lindblad P. Transcriptional regulation of the cyanobacterial bidirectional Hox-hydrogenase. Dalton Trans. 2009, 9990.
74. Perovic S., Seack J., Gamulin V., Muller W., Schroder H.C. Modulation of intracellular calcium and proliferative activity of invertebrate and vertebrate cells by ethylene. BMC Cell Biol. 2001, 2. art. no.-7.
75. Prabaharan D., Kumar D.A., Uma L., Subramanian G. Dark hydrogen production in nitrogen atmosphere - an approach for sustainability by marine cyanobacterium Leptolyngbya valderiana BDU 20041. Int. J. Hydrogen Energy 2010, 35:10725-10730.
76. Ray D.K., Ramankutty N., Mueller N.D., West P.C., Foley J.A. Recent patterns of crop yield growth and stagnation. Nat. Commun. 2012, 3:1293.
77. Saini V., Sigman M.S. Palladium-catalyzed 1,1-difunctionalization of ethylene. J. Am. Chem. Soc. 2012, 134:11372-11375.
78. Sakai M., Ogawa T., Matsuoka M., Fukuda H. Photosynthetic conversion of carbon dioxide to ethylene by the recombinant cyanobacterium, Synechococcus sp. PCC 7942, which harbors a gene for the ethylene-forming enzyme of Pseudomonas syringae. J. Ferment. Bioeng. 1997, 84:434-443.
79. Sakurai H., Masukawa H. Promoting R & D in photobiological hydrogen production utilizing mariculture-raised cyanobacteria. Mar. Biotechnol. 2007, 9:128-145.
80. Schirmer A., Rude M.A., Li X., Popova E., del Cardayre S.B. Microbial biosynthesis of alkanes. Science 2010, 329:559-562.
81. Schmitz O., Boison G., Bothe H. Quantitative analysis of expression of two circadian clock-controlled gene clusters coding for the bidirectional hydrogenase in the cyanobacterium Synechococcus sp. PCC7942. Mol. Microbiol. 2001, 41:1409-1417.
82. Schwarz C., Poss Z., Hoffmann D., Appel J. Hydrogenases and Hydrogen Metabolism in Photosynthetic Prokaryotes 2010, vol. 675:305-348. SPRINGER-VERLAG BERLIN, Heidelberger Platz 3, D-14197, BERLIN, GERMANY.
83. Seabra R., Santos A., Pereira S., Moradas-Ferreira P., Tamagnini P. Immunolocalization of the uptake hydrogenase in the marine cyanobacterium Lyngbya majuscula CCAP 1446/4 and two Nostoc strains. FEMS Microbiol. Lett. 2009, 292:57-62.
84. Sharkey T.D. Emission of low molecular mass hydrocarbons from plants. Trends Plant Sci. 1996, 1:78-82.
85. Sharkey T.D., Chen X.Y., Yeh S. Isoprene increases thermotolerance of fosmidomycin-fed leaves. Plant Physiol. 2001, 125:2001-2006.
86. Sheehan J. Engineering direct conversion of CO2 to biofuel. Nat. Biotechnol. 2009, 27:1128-1129.
87. Shestako S., Khyen N.T. Evidence for genetic transformation in blue-green alga Anacystis nidulans. Mol. Gen. Genet. 1970, 107:372.
88. Silver G.M., Fall R. Enzymatic synthesis of isoprene from dimethylallyl diphosphate in aspen leaf extracts. Plant Physiol. 1991, 97:1588-1591.
89. Silver G.M., Fall R. Characterization of aspen isoprene synthase, an enzyme responsible for leaf isoprene emission to the atmosphere. J. Biol. Chem. 1995, 270:13010-13016.
90. Summers M.L., Wallis J.G., Campbell E.L., Meeks J.C. Genetic evidence of a major role for glucose-6-phosphate-dehydrogenase in nitrogen-fixation and dark growth of the cyanobacterium Nostoc sp strain Atcc-29133. J. Bacteriol. 1995, 177:6184-6194.
91. Taiz L., Zeiger E. Plant Physiology 2002, 672. Sinauer Associates, Sunderland, MA. third ed. L. Taiz (Ed.).
92. Takahama K., Matsuoka M., Nagahama K., Ogawa T. Construction and analysis of a recombinant cyanobacterium expressing a chromosomally inserted gene for an ethylene-forming enzyme at the psbAI locus. J. Biosci. Bioeng. 2003, 95:302-305.
93. Tamagnini P., Leitao E., Oliveira P., Ferreira D., Pinto F., Harris D.J., Heidorn T., Lindblad P. Cyanobacterial hydrogenases: diversity, regulation and applications. FEMS Microbiol. Rev. 2007, 31:692-720.
94. Tan X., Yao L., Gao Q., Wang W., Qi F., Lu X. Photosynthesis driven conversion of carbon dioxide to fatty alcohols and hydrocarbons in cyanobacteria. Metab. Eng. 2011, 13:169-176.
95. Tao L., Dong H.-J., Chen X., Chen S.-F., Wang T.-H. Expression of ethylene-forming enzyme (EFE) of Pseudomonas syringae pv. glycinea in Trichoderma viride. Appl. Microbiol. Biotechnol. 2008, 80:573-578.
96. Thiel T., Poo H. Transformation of a filamentous cyanobacterium by electroporation. J. Bacteriol. 1989, 171:5743-5746.
97. Troshina O., Serebryakova L., Sheremetieva M., Lindblad P. Production of H-2 by the unicellular cyanobacterium Gloeocapsa alpicola CALU 743 during fermentation. International Journal of Hydrogen Energy 2002, 27:1283-1289.
98. Tsinoremas N.F., Kutach A.K., Strayer C.A., Golden S.S. Efficient gene-transfer in Synechococcus sp strains Pcc-7942 and Pcc-6301 by Interspecies conjugation and chromosomal recombination. J. Bacteriol. 1994, 176:6764-6768.
99. Tsygankov A.A., Fedorov A.S., Kosourov S.N., Rao K.K. Hydrogen production by cyanobacteria in an automated outdoor photobioreactor under aerobic conditions. Biotechnol. Bioeng. 2002, 80:777-783.
100. 2 to ethylene in recombinant cyanobacterium Synechocystis 6803. Energy Environ. Sci. 2012, 5:8998-9006.
101. Verne, J. The Mysterious Island. Plain Label Books.
102. Vignais P.M., Billoud B. Occurrence, classification, and biological function of hydrogenases: an overview. Chem. Rev. 2007, 107:4206-4272.
103. Wang C.H., Persyn A., Krackov J. Role of the Krebs cycle in ethylene biosynthesis. Nature 1962, 195:1306-1308.
104. Wang K., Li H., Ecker J.R. Ethylene biosynthesis and signaling networks. Plant Cell 2002, 14:S131-S151.
105. Weingart H., Völksch B., Ullrich M.S. Comparison of ethylene production by Pseudomonas syringae and Ralstonia solanacearum. Phytopathology 1999, 89:360-365.
106. Weissman J.C., Benemann J.R. Hydrogen production by nitrogen-starved cultures of Anabaena cylindrica. Appl. Environ. Microbiol. 1977, 33:123-131.
107. Weyman P.D., Pratte B., Thiel T. Transcription of hupSL in Anabaena variabilis ATCC 29413 is regulated by NtcA and Not by Hydrogen. Appl. Environ. Microbiol. 2008, 74:2103-2110.
108. Winters K., Parker P.L., Vanbaale C. Hydrocarbons of blue-green algae - geochemical significance. Science 1969, 163:467.
109. Withers S.T., Gottlieb S.S., Lieu B., Newman J.D., Keasling J.D. Identification of isopentenol biosynthetic genes from Bacillus subtilis by a screening method based on isoprenoid precursor toxicity. App. Environ. Microbiol. 2007, 73:6277-6283.
110. Wolk C.P., Vonshak A., Kehoe P., Elhai J. Construction of shuttle vectors capable of conjugative transfer from Escherichia coli to nitrogen-fixing filamentous cyanobacteria. Proc. Natl. Acad. Sci. USA. 1984, 81:1561-1565.
111. Xue J., Ahring B.K. Enhancing isoprene production by genetic modification of the 1-Deoxy-D-Xylulose-5-Phosphate pathway in Bacillus subtilis. Appl. Environ. Microbiol. 2011, 77:2399-2405.
112. Yang J., Zhao G., Sun Y., Zheng Y., Jiang X., Liu W., Xian M. Bio-isoprene production using exogenous MVA pathway and isoprene synthase in Escherichia coli. Bioresour. Technol. 2012, 104:642-647.
113. Yoon H.S. A molecular timeline for the origin of photosynthetic eukaryotes. Mol. Biol. Evol. 2004, 21:809-818.
114. Yoon H.S., Haeshin J., Kim M., Junsim S., Park T. Evaluation of conversion efficiency of light to hydrogen energy by Anabaena variabilis. Int. J. Hydrogen Energy 2006, 31:721-727.
115. Yoshihara S., Geng X., Okamoto S., Yura K., Murata T., Go M., Ohmori M., Ikeuchi M. Mutational analysis of genes involved in pilus structure, motility and transformation competency in the unicellular motile cyanobacterium Synechocystis sp. PCC 6803. Plant Cell. Physiol. 2001, 42:63-73.
116. Yura K. Putative mechanism of natural transformation as deduced from genome data. DNA Res. 1999, 6:75-82.
117. Zang X., Liu B., Liu S., Arunakumara K.K.I.U., Zhang X. Optimum conditions for transformation of Synechocystis sp. PCC 6803. J. Microbiol. 2007, 45:241-245.
118. Zhang S., Bryant D.A. The Tricarboxylic Acid Cycle in Cyanobacteria. Science 2011, 334(6062):1551-1553.
119. Zhao Y., Yang J., Qin B., Li Y., Sun Y., Su S., Xian M. Biosynthesis of isoprene in Escherichia coli via methylerythritol phosphate (MEP) pathway. Appl. Microbiol. Biotechnol. 2011, 90:1915-1922.