Construction and analysis of a recombinant cyanobacterium expressing a chromosomally inserted gene for an ethylene-forming enzyme at the psbAI locus

Journal of Bioscience and Bioengineering

Volumn 95, Issue 3, 2003, Pages 302-305

  Takahama, Kazutaka ^a   Matsuoka, Masayoshi ^a   Nagahama, Kazuhiro ^a   Ogawa, Takahira ^a  

^a SOJO UNIVERSITY   (Japan)

Author keywords

2 oxoglutarate; Ethylene; Gene replacement; Metabolic stress; rps12; Synechococcus elongatus

Indexed keywords

CHROMOSOMES; ETHYLENE; GENES;

CYANOBACTERIUM;

ENZYMES;

BACTERIAL ENZYME; ETHYLENE;

ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL METABOLISM; BACTERIAL STRAIN; BACTERIUM MUTANT; CONTROLLED STUDY; CYANOBACTERIUM; GENE CONSTRUCT; GENE INSERTION; GENE LOCUS; GENE SEQUENCE; GENE TRANSFER; GENETIC CODE; GENETIC RECOMBINATION; GRAM NEGATIVE BACTERIUM; GROWTH RATE; NONHUMAN; PHENOTYPE; PSBAI GENE; PSEUDOMONAS SYRINGAE; STRESS;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); INSERTION SEQUENCES; NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS SYRINGAE; SYNECHOCOCCUS; SYNECHOCOCCUS ELONGATUS; SYNECHOCOCCUS ELONGATUS PCC 7942; SYNECHOCOCCUS SP. PCC 6301; UNCULTURED ACTINOMYCETE;

EID: 0037265743     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.95.302     Document Type: Article

References (13)

1. Fukuda, H., Ogawa, T., Tazaki, M., Nagahama, K., Fujii, T., Tanase, S., and Morino, Y.: Two reactions are simultaneously catalyzed by a single enzyme: the arginine-dependent simultaneous formation of two products, ethylene and succinate, from 2-oxoglutarate by an enzyme from Pseudomonas syringae. Biochem. Biophys. Res. Commun., 188, 483-489 (1992).
2. Nagahama, K., Ogawa, T., Fujii, T., Tazaki, M., Tanase, S., Morino, Y., and Fukuda, H.: Purification and properties of an ethylene-forming enzyme from Pseudomonas syringae pv. phaseolicola PK2. J. Gen. Microbiol., 137, 2281-2286 (1991).
3. Fukuda, H., Ogawa, T., Ishihara, K., Fujii, T., Nagahama, K., Omata, T., Inoue, Y., Tanase, S., and Morino, Y.: Molecular cloning in Escherichia coli, expression, and nucleotide sequence of the gene for the ethylene-forming enzyme of Pseudomonas syringae pv. phaseolicola PK2. Biochem. Biophys. Res. Commun., 188, 826-832 (1992).
4. Kuhlemeier, C. J., Thomas, A. A. M., van der Ende, A., van Leen, R. W., Borrias, W. E., van den Hondel, C. A. M. J. J., and van Arkel, G. A.: A host-vector system for gene cloning in the cyanobacterium Anacystis nidulans R2. Plasmid, 10, 156-163 (1983).
5. Fukuda, H., Sakai, M., Nagahama, K., Fujii, T., Matsuoka, M., Inoue, Y., and Ogawa, T.: Heterologous expression of the gene for the ethylene-forming enzyme from Pseudomonas syringae in the cyanobacterium Synechococcus. Biotechnol. Lett., 16, 1-6 (1994).
6. Golden, S. S., Brusslan, J., and Haselkorn, R.: Expression of a family of psbA genes encoding a photosystem 11 polypeptide in the cyanobacterium Anacystis nidulans R2. EMBO J., 5, 2789-2798 (1986).
7. Nair, U., Thomas, C., and Golden, S. S.: Functional elements of the strong psbAI promoter of Synechococcus elongates PCC 7942. J. Bacteriol., 183, 1740-1747 (2001).
8. Sakai, M., Ogawa, T., Matsuoka, M., and Fukuda, H.: Photosynthetic conversion of carbon dioxide to ethylene by the recombinant cyanobacterium, Synechococcus sp. PCC 7942, which harbors a gene for the ethylene-forming enzyme of Pseudomonas syringae. J. Ferment. Bioeng., 84, 434-443 (1997).
9. Matsuoka, M., Takahama, K., and Ogawa, T.: Gene replacement in cyanobacteria mediated by a dominant streptomycin-sensitive rps12 gene that allows selection of mutants free from drug resistance markers. Microbiology, 147, 2077-2087 (2001).
10. Rippka, R.: Isolation and purification of cyanobacteria. Methods Enzymol., 167, 3-28 (1988).
11. van der Plas, J., Oosterhoff-Teertstra, R., Borrias, M., and Weisbeek, P.: Identification of replication and stability functions in the complete nucleotide sequence of plasmid pUH24 from the cyanobacterium Synechococcus sp. PCC 7942. Mol. Microbiol., 6, 653-664 (1992).
12. Wang, J., Araki, T., Ogawa, T., Matsuoka, M., and Fukuda, H.: A method of graphically analyzing substrate-inhibition kinetics. Biotechnol. Bioeng., 62, 402-411 (1999).
13. Grimm, B., Bull, A., and Breu, V.: Structural genes of glutamate 1-semialdehyde aminotransferase for porphyrin synthesis in a cyanobacterium and Escherichia coli. Mol. Gen. Genet., 225, 1-10 (1991).