Bufalin inhibited the growth of human osteosarcoma MG-63 cells via down-regulation of Bcl-2/Bax and triggering of the mitochondrial pathway

Tumor Biology

Volumn 35, Issue 5, 2014, Pages 4885-4890

  Wang, Dewei ^a   Bi, Zhenggang ^a  

^a THE FIRST AFFILIATED HOSPITAL OF HARBIN MEDICAL UNIVERSITY   (China)

Author keywords

Apoptosis; Bufalin; Mitochondrial transmembrane potential; Osteosarcoma

Indexed keywords

APOPTOTIC PROTEASE ACTIVATING FACTOR 1; BETA ACTIN; BUFALIN; CASPASE 3; CASPASE 7; CASPASE 9; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; PROTEIN BAX; PROTEIN BCL 2; REACTIVE OXYGEN METABOLITE; ANTINEOPLASTIC AGENT; BAX PROTEIN, HUMAN; BUFADIENOLIDE DERIVATIVE;

APOPTOSIS; ARTICLE; CANCER INHIBITION; CELL PROLIFERATION; CONTROLLED STUDY; DOSE TIME EFFECT RELATION; DOWN REGULATION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MITOCHONDRIAL MEMBRANE POTENTIAL; MITOCHONDRION; MTT ASSAY; OSTEOSARCOMA; OSTEOSARCOMA CELL LINE; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN INTERACTION; UPREGULATION; WESTERN BLOTTING; BONE TUMOR; DRUG ANTAGONISM; DRUG EFFECT; METABOLISM; PATHOLOGY; TUMOR CELL LINE;

ANTINEOPLASTIC AGENTS; APOPTOSIS; BCL-2-ASSOCIATED X PROTEIN; BONE NEOPLASMS; BUFANOLIDES; CELL LINE, TUMOR; CELL PROLIFERATION; DOWN-REGULATION; HUMANS; MEMBRANE POTENTIAL, MITOCHONDRIAL; MITOCHONDRIA; OSTEOSARCOMA; PROTO-ONCOGENE PROTEINS C-BCL-2; REACTIVE OXYGEN SPECIES;

EID: 84902974693     PISSN: 10104283     EISSN: 14230380     Source Type: Journal    
DOI: 10.1007/s13277-014-1640-7     Document Type: Article

References (25)

1. Thompson Jr RC, Cheng EY, Clohisy DR, Perentesis J, Manivel C, Le CT. Clin Orthop Relat Res. 2002;397:240-347.
2. Arndt CA, Crist WM. N Engl J Med. 1999;341:342-52.
3. Bacci G, Longhi A, Bertoni F, Bacchini P, Ruggeri P, Versari M, et al. Primary high-grade osteosarcoma: comparison between preadolescent and older patients. J Pediatr Hematol Oncol. 2005;27:129-34. (Pubitemid 40397241)
4. Yang C, Choy E, Hornicek FJ, Wood KB, Schwab JH, Liu X, et al. Histone deacetylase inhibitor (HDACI) PCI-24781 potentiates cytotoxic effects of doxorubicin in bone sarcoma cells. Cancer Chemother Pharmacol. 2011;67:439-46.
5. Wittenburg LA, Bisson L, Rose BJ, Korch C, Thamm DH. The histone deacetylase inhibitor valproic acid sensitizes human and canine osteosarcoma to doxorubicin. Cancer Chemother Pharmacol. 2011;67:83-92.
6. Qi F, Li A, Inagaki Y, Kokudo N, Tamura S, Nakata M, et al. Antitumor activity of extracts and compounds from the skin of the toad Bufo bufo gargarizans Cantor. Int Immunopharmacol. 2011;11:342-9.
7. Qi F, Inagaki Y, Gao B, Cui X, Xu H, Kokudo N, et al. Bufalin and cinobufagin induce apoptosis of human hepatocellular carcinoma cells via Fas- and mitochondria-mediated pathways. Cancer Sci. 2011;102:951-8.
8. Li H, Wang P, Gao Y, Zhu X, Liu L, Cohen L, et al. Na+/K+-ATPase α3 mediates sensitivity of hepatocellular carcinoma cells to bufalin. Oncol Rep. 2011;25:825-30.
9. Xie CM, Chan WY, Yu S, Zhao J, Cheng CH. Bufalin induces autophagy-mediated cell death in human colon cancer cells through reactive oxygen species generation and JNK activation. Free Radic Biol Med. 2011;51:1365-75.
10. Amano Y, Cho Y, Matsunawa M, Komiyama K, Makishima M. Increased nuclear expression and transactivation of vitamin D receptor by the cardiotonic steroid bufalin in human myeloid leukemia cells. J Steroid Biochem Mol Biol. 2009;114:144-51.
11. Li D, Qu X, Hou K, Zhang Y, Dong Q, Teng Y, et al. PI3K/Akt is involved in bufalin induced apoptosis in gastric cancer cells. Anticancer Drugs. 2009;20:59-64.
12. Yu CH, Kan SF, Pu HF, Jea Chien E, Wang PS. Apoptotic signaling in bufalin- and cinobufagin-treated and rogen-dependent and - independent human prostate cancer cells. Cancer Sci. 2008;99:2467-76.
13. Hsiao YP, Yu CS, Yu CC, Yang JS, Chiang JH, Lu CC, et al. Triggering apoptotic death of human malignant melanoma A375.S2 cells by bufalin: involvement of caspase cascade-dependent and independent mitochondrial signaling pathways. Evid Based Complement Alternat Med. 2012;2012:591241.
14. Jiang Y, Zhang Y, Luan J, Duan H, Zhang F, Yagasaki K, et al. Effects of bufalin on the proliferation of human lung cancer cells and its molecular mechanisms of action. Cytotechnology. 2010;62:573-83.
15. Zhu Z, Sun H, Ma G, Wang Z, Li E, Liu Y, et al. Bufalin induces lung cancer cell apotosis via the inhibition of PI3K/Akt pathway. Int J Mol Sci. 2012;13:2025-35.
16. Ek ET, Choong PF. The role of high-dose therapy and autologous stem cell transplantation for pediatric bone and soft tissue sarcomas. Expert Rev Anticancer Ther. 2006;6:225-37. (Pubitemid 44192934)
17. Yang C, Hornicek FJ, Wood KB, Schwab JH, Mankin H, Duan Z. RAIDD expression is impaired in multidrug resistant osteosarcoma cell lines. Cancer Chemother Pharmacol. 2009;64:607-14.
18. Sun SY, Hail Jr N, Lotan R. Apoptosis as a novel target for cancer chemoprevention. J Natl Cancer Inst. 2004;96:662-72. (Pubitemid 38660229)
19. Hirsch T, Marzo I, Kroemer G. Role of the mitochondrial permeability transition pore in apoptosis. Biosci Rep. 1997;17:67-76. (Pubitemid 27267593)
20. Iannolo G, Conticello C, Memeo L, DeMaria R. Apoptosis in normal and cancer stem cells. Crit Rev Oncol Hematol. 2008;66:42-51. (Pubitemid 351330196)
21. Mohamad N, Gutierrez A, Nunez M, Cocca C, Martín G, Cricco G, et al. Mitochondrial apoptotic pathways. Biocell. 2005;29:149-61. (Pubitemid 41297442)
22. Orrenius S, Gogvadze V, Zhivotovsky B. Mitochondrial oxidative stress: implications for cell death. Annu Rev Pharmacol Toxicol. 2007;47:143-83.
23. Yin XM. Signal transduction mediated by Bid, a pro-death Bcl-2 family proteins, connects the death receptor and mitochondria apoptosis pathways. Cell Res. 2000;10:161-7.
24. Takahashi A, Masuda A, Sun M, Centonze VE, Herman B. Oxidative stress-induced apoptosis is associated with alterations in mitochondrial caspase activity and Bcl-2-dependent alterations in mitochondrial pH (pHm). Brain Res Bull. 2004;62:497-504. (Pubitemid 38253035)
25. 2+ - calpain and caspase-mitochondriadependent pathways. J Biol Chem. 2000;275:39702-9.