A simple identification method for vaginal secretions using relative quantification of Lactobacillus DNA

Forensic Science International: Genetics

Volumn 12, Issue , 2014, Pages 93-99

  Doi, Masanori ^a   Gamo, Shinsuke ^a   Okiura, Tatsuyuki ^a,b   Nishimukai, Hiroaki ^b   Asano, Migiwa ^b  

^a Forensic Science Laboratory Ehime Prefectural Police Headquarters   (Japan)

^b EHIME UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

Author keywords

Body fluid identification; Human DNA; Lactobacillus; Microflora; Real time PCR; Vaginal secretions

Indexed keywords

BACTERIAL DNA; BACTERIAL RNA; RNA 16S; PRIMER DNA;

ARTICLE; BODY FLUID; CONTROLLED STUDY; DNA DETERMINATION; FEMALE; FORENSIC IDENTIFICATION; GENE SEQUENCE; HUMAN; LACTOBACILLUS; LACTOBACILLUS CRISPATUS; LACTOBACILLUS GASSERI; LACTOBACILLUS INERS; LACTOBACILLUS JENSENII; MALE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; ROOM TEMPERATURE; SALIVA; SKIN SURFACE; SPECIES IDENTIFICATION; SPERM; STORAGE TEMPERATURE; VAGINAL SECRETION; GENETICS; MICROBIOLOGY; NUCLEOTIDE SEQUENCE; SECRETION (PROCESS); VAGINA;

BASE SEQUENCE; DNA PRIMERS; DNA, BACTERIAL; FEMALE; HUMANS; LACTOBACILLUS; REAL-TIME POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; VAGINA;

EID: 84901976189     PISSN: 18724973     EISSN: 18780326     Source Type: Journal    
DOI: 10.1016/j.fsigen.2014.05.005     Document Type: Article

References (39)

1. J. Juusola, and J. Ballantyne Messenger RNA profiling: a prototype method to supplant conventional methods for body fluid identification Forensic Sci. Int. 135 2003 85 96 (Pubitemid 36960213)
2. C. Nussbaumer, E. Gharehbaghi-Schnell, and I. Korschineck Messenger RNA profiling: a novel method for body fluid identification by real-time PCR Forensic Sci. Int. 157 2006 181 186 (Pubitemid 43243177)
3. J. Juusola, and J. Ballantyne mRNA profiling for body fluid identification by multiplex quantitative RT-PCR J. Forensic Sci. 52 2007 1252 1262 (Pubitemid 350243424)
4. C. Haas, B. Klesser, C. Maake, W. Bar, and A. Kratzer mRNA profiling for body fluid identification by reverse transcription endpoint PCR and real time PCR Forensic Sci. Int. Genet. 3 2009 80 88
5. B. Liu, J.R. Lague, D.P. Nunes, P. Toselli, F.G. Oppenheim, and R.V. Soares et al. Expression of membrane-associated mucins MUC1 and MUC4 in major human salivary glands J. Histochem. Cytochem. 50 2002 811 820 (Pubitemid 34721449)
6. Y. Abiko, M. Nishimura, and T. Kaku Defensins in saliva and the salivary glands Med. Electron Microsc. 36 2003 247 252 (Pubitemid 38145543)
7. M.L. Richard, K.A. Harper, R.L. Craig, A.J. Onorato, J.M. Robertson, and J. Donfack Evaluation of mRNA marker specificity for the identification of five human body fluids by capillary electrophoresis Forensic Sci. Int. Genet. 6 2012 452 460
8. S.M. Park, S.Y. Park, J.H. Kim, T.W. Kang, J.L. Park, and K.M. Woo Genome-wide mRNA profiling and multiplex quantitative RT-PCR for forensic body fluid identification Forensic Sci. Int. Genet. 7 2013 143 150
9. H. Nakanishi, A. Kido, T. Ohmori, A. Takada, M. Hara, and N. Adachi et al. A novel method for the identification of saliva by detecting oral streptococci using PCR Forensic Sci. Int. 183 2009 20 23
10. H. Nakanishi, H. Shojo, T. Ohmori, M. Hara, A. Takada, and N. Adachi et al. Identification of feces by detection of Bacteroides genes Forensic Sci. Int. Genet. 7 2013 176 179
11. R.I. Fleming, and S. Harbison The use of bacteria for the identification of vaginal secretions Forensic Sci. Int. Genet. 4 2010 311 315
12. T. Akutsu, H. Motani, K. Watanabe, H. Iwase, and K. Sakurada Detection of bacterial 16S ribosomal RNA genes for forensic identification of vaginal fluid Leg. Med. (Tokyo) 14 2012 160 162
13. S. Giampaoli, A. Berti, F. Valeriani, G. Gianfranceschi, A. Piccolella, and L. Buggiotti et al. Molecular identification of vaginal fluid by microbial signature Forensic Sci. Int. Genet. 6 2012 559 564
14. V. Redondo-Lopez, R.L. Cook, and J.D. Sobel Emerging role of lactobacilli in the control and maintenance of the vaginal bacterial microflora Rev. Infect. Dis. 12 1990 856 872 (Pubitemid 20349968)
15. S.I. Pavlova, A.O. Kilic, S.S. Kilic, J.S. So, M.E. Nader-Macias, and J.A. Simoes et al. Genetic diversity of vaginal lactobacilli from women in different countries based on 16S rRNA gene sequences J. Appl. Microbiol. 92 2002 451 459 (Pubitemid 34227136)
16. X. Zhou, M.A. Hansmann, C.C. Davis, H. Suzuki, C.J. Brown, and U. Schutte et al. The vaginal bacterial communities of Japanese women resemble those of women in other racial groups FEMS Immunol. Med. Microbiol. 58 2010 169 181
17. V. Jespers, J. Menten, H. Smet, S. Poradosu, S. Abdellati, and R. Verhelst et al. Quantification of bacterial species of the vaginal microbiome in different groups of women, using nucleic acid amplification tests BMC Microbiol. 12 2012 83
18. R. Byun, M.A. Nadkarni, K.L. Chhour, F.E. Martin, N.A. Jacques, and N. Hunter Quantitative analysis of diverse Lactobacillus species present in advanced dental caries J. Clin. Microbiol. 42 2004 3128 3136 (Pubitemid 38938069)
19. I. Sakamoto, M. Igarashi, K. Kimura, A. Takagi, T. Miwa, and Y. Koga Suppressive effect of Lactobacillus gasseri OLL 2716 (LG21) on Helicobacter pylori infection in humans J. Antimicrob. Chemother. 47 2001 709 710 (Pubitemid 32447667)
20. C.C. Benschop, F.C. Quaak, M.E. Boon, T. Sijen, and I. Kuiper Vaginal microbial flora analysis by next generation sequencing and microarrays; can microbes indicate vaginal origin in a forensic context? Int. J. Leg. Med. 126 2012 303 310
21. K. Tamura, J. Dudley, M. Nei, and S. Kumar MEGA4. Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0 Mol. Biol. Evol. 24 2007 1596 1599 (Pubitemid 47236692)
22. J. Rozas, J.C. Sanchez-DelBarrio, X. Messeguer, and R. Rozas DnaSP, DNA polymorphism analyses by the coalescent and other methods Bioinformatics 19 2003 2496 2497 (Pubitemid 38016658)
23. H. Nakahara, K. Fujii, N. Mizuno, K. Yoshida, and K. Kasai Evaluations of DNA quantification methods for forensic biological samples Jpn. J. Forensic Sci. Technol. 12 2007 13 26
24. H. Nakahara, N. Mizuno, K. Fujii, and K. Sekiguchi Human DNA specific detection from forensic biological samples Rep. Natl. Res. Inst. Police Sci. 58 2007 66 74
25. P.S. Walsh, J. Varlaro, and R. Reynolds A rapid chemiluminescent method for quantitation of human DNA Nucleic Acids Res. 20 1992 5061 5065
26. T. Nakazono, S. Kashimura, Y. Hayashiba, K. Hara, and A. Miyoshi Successful DNA typing of urine stains using a DNA purification kit following dialfiltration J. Forensic Sci. 50 2005 860 864 (Pubitemid 40917801)
27. X. Zhou, S.J. Bent, M.G. Schneider, C.C. Davis, M.R. Islam, and L.J. Forney Characterization of vaginal microbial communities in adult healthy women using cultivation-independent methods Microbiology 150 2004 2565 2573 (Pubitemid 39173262)
28. S.S. Witkin, I.M. Linhares, and P. Giraldo Bacterial flora of the female genital tract: function and immune regulation Best Pract. Res. Clin. Obstet. Gynaecol. 21 2007 347 354 (Pubitemid 46825481)
29. C. Haas, E. Hanson, W. Bar, R. Banemann, A.M. Bento, and A. Berti et al. mRNA profiling for the identification of blood - results of a collaborative EDNAP exercise Forensic Sci. Int. Genet. 5 2011 21 26
30. C. Haas, E. Hanson, M.J. Anjos, W. Bar, R. Banemann, and A. Berti et al. RNA/DNA co-analysis from blood stains - results of a second collaborative EDNAP exercise Forensic Sci. Int. Genet. 6 2012 70 80
31. C. Haas, E. Hanson, M.J. Anjos, R. Banemann, A. Berti, and E. Borges et al. RNA/DNA co-analysis from human saliva and semen stains - results of a third collaborative EDNAP exercise Forensic Sci. Int. Genet. 7 2013 230 239
32. M.J. Claesson, D. van Sinderen, and P.W. O'Toole The genus Lactobacillus - a genomic basis for understanding its diversity FEMS Microbiol. Lett. 269 2007 22 28 (Pubitemid 46354133)
33. C.J. Priestley, B.M. Jones, J. Dhar, and L. Goodwin What is normal vaginal flora? Genitourin. Med. 73 1997 23 28 (Pubitemid 27076083)
34. D.A. Eschenbach, S.S. Thwin, D.L. Patton, T.M. Hooton, A.E. Stapleton, and K. Agnew et al. Influence of the normal menstrual cycle on vaginal tissue, discharge, and microflora Clin. Infect. Dis. 30 2000 901 907 (Pubitemid 32250327)
35. J.R. Schwebke, C.M. Richey, and H.L. Weiss2 Correlation of behaviors with microbiological changes in vaginal flora J. Infect. Dis. 180 1999 1632 1636 (Pubitemid 29506468)
36. J. Jakubowska, A. Maciejewska, R. Pawlowski, and K.P. Bielawski mRNA profiling for vaginal fluid and menstrual blood identification Forensic Sci. Int. Genet. 7 2013 272 278
37. A. Choi, K.J. Shin, W.I. Yang, and H.Y. Lee Body fluid identification by integrated analysis of DNA methylation and body fluid-specific microbial DNA Int. J. Leg. Med. 128 2014 33 41
38. E.K. Hanson, and J. Ballantyne Highly specific mRNA biomarkers for the identification of vaginal secretions in sexual assault investigations Sci. Justice 53 2013 14 22
39. C. Haas, E. Hanson, M.J. Anjos, K.N. Ballantyne, R. Banemann, and B. Bhoelai et al. RNA/DNA co-analysis from human menstrual blood and vaginal secretion stains: results of a fourth and fifth collaborative EDNAP exercise Forensic Sci. Int. Genet. 8 2014 203 212