Molecular identification of vaginal fluid by microbial signature

Forensic Science International: Genetics

Volumn 6, Issue 5, 2012, Pages 559-564

  Giampaoli, Saverio ^a   Berti, Andrea ^b   Valeriani, Federica ^a   Gianfranceschi, Gianluca ^a   Piccolella, Antonio ^a   Buggiotti, Laura ^a   Rapone, Cesare ^b   Valentini, Alessio ^c   Ripani, Luigi ^b   Romano Spica, Vincenzo ^a  

^a UNIVERSITY OF ROME FORO ITALICO   (Italy)

^b Reparto Investigazioni Scientifiche di Roma   (Italy)

^c UNIVERSITY OF TUSCIA   (Italy)

Author keywords

Bacteria; Forensic; mfDNA; Multiplex PCR; Vaginal fluid

Indexed keywords

BACTERIAL DNA; OLIGONUCLEOTIDE;

ADULT; AGED; ARTICLE; BACTERIAL GENOME; CONTROLLED STUDY; DNA EXTRACTION; ENTEROCOCCACEAE; FECES ANALYSIS; FEMALE; FEMALE GENITAL SYSTEM; GENE TARGETING; HUMAN; MICROFLORA; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SALIVA ANALYSIS; VAGINA DISCHARGE;

BASE SEQUENCE; BODY FLUIDS; DNA PRIMERS; DNA, BACTERIAL; FEMALE; HUMANS; MICROSATELLITE REPEATS; REAL-TIME POLYMERASE CHAIN REACTION; VAGINA;

EID: 84863780398     PISSN: 18724973     EISSN: 18780326     Source Type: Journal    
DOI: 10.1016/j.fsigen.2012.01.005     Document Type: Article

References (33)

1. L. Quarino, Q. Dang, J. Hartmann, N. Moynihan, An ELISA method for the identification of salivary amylase, J. Forensic Sci. 50 (2005) 873-876. (Pubitemid 40917803)
2. H. Tsutsumi, K. Higashide, Y. Mizuno, K. Tamaki, Y. Katsumata, Identification of saliva stains by determination of the specific activity of amylase, Forensic Sci. Int. 50 (1991) 37-42.
3. B.C. Pang, B.K. Cheung, Identification of human semenogelin in membrane strip test as an alternative method for the detection of semen, Forensic Sci. Int. 169 (2007) 27-31. (Pubitemid 46702437)
4. E. Marvez-Valls, D. Travis, T. Savadore, M. Haydel, OneStep ABAcard p30 test for the identification of semen in sexual assault victims, Acad. Emerg. Med. 10 (2003) 564.
5. A. Castello, F. Frances, F. Verdú, An alternative to the human hemoglobin test in the investigation of bloodstains treated with active oxygen: the human glycophorin a test, Sci. World J. 11 (2011) 907-916.
6. S. Johnston, J. Newman, R. Frappier, Validation study of the Abacus Diagnostics ABAcard® Hematrace® membrane test for the forensic identification of human blood, Can. Soc. Forensic Sci. J. 36 (2003) 173-183. (Pubitemid 38198537)
7. T. Akutsu, H. Ikegaya, K. Watanabe, H. Fukushima, H. Motani, H. Iwase, K. Sakurada, Evaluation of Tamm-Horsfall protein and uroplakin III for forensic identification of urine, J. Forensic Sci. 55 (2010) 742-746.
8. A.J. Peabody, R.M. Burgess, R.E. Stockdale, Re-examination of the Lugol's Iodine Test, Home Office Central Research Establishment, 1981 (Report no. 412).
9. T.J. Rothwell, K.J. Harvey, The limitations of the Lugol's iodine staining technique for the identification of vaginal epithelial cells, J. Forensic Sci. Soc. 18 (1978) 181-184. (Pubitemid 9233512)
10. R. Hausmann, B. Schellmann, Forensic value of the Lugol's staining method: further studies on glycogenated epithelium in the male urinary tract, Int. J. Legal Med. 107 (1994) 147-151. (Pubitemid 24380851)
11. C. Haas, E. Hanson, M.J. Anjos, W. Bär, R. Banemann, A. Berti, E. Borges, C. Bouakaze, A. Carracedo,M. Carvalho, V. Castella, A. Choma, G. De Cock, M. Dötsch, P. Hoff-Olsen, P. Johansen, F. Kohlmeier, P.A. Lindenbergh, B. Ludes, O. Maroñas, D. Moore, M.L. Morerod, N. Morling, H. Niederstätter, F. Noel,W. Parson, G. Patel, C. Popielarz, E. Salata, P.M. Schneider, T. Sijen, B. Sviežena, M. Turanská, L. Zatkalíková, J. Ballantyne, RNA/DNA co-analysis from blood stains-results of a second collaborative EDNAP exercise, Forensic Sci. Int. Genet. (2011), doi:10.1016/j.fsigen.2011.02.004.
12. C. Nussbaumer, E. Gharehbaghi-Schnell, I. Korschineck, Messenger RNA profiling: a novel method for body fluid identification by real-time PCR, Forensic Sci. Int. 157 (2006) 181-186. (Pubitemid 43243177)
13. J. Juusola, J. Ballantyne, mRNA profiling for body fluid identification by multiplex quantitative RT-PCR, J. Forensic Sci. 52 (2007) 1252-1262. (Pubitemid 350243424)
14. M. Setzer, J. Juusola, J. Ballantyne, Recovery and stability of RNA in vaginal swabs and blood, semen, and saliva stains, J. Forensic Sci. 53 (2008) 296-305. (Pubitemid 351432610)
15. D. Zubakov, M. Kokshoorn, A. Kloosterman, M. Kayser, New markers for old stains: stable mRNA markers for blood and saliva identification from up to 16-year-old stains, Int. J. Legal Med. 123 (2009) 71-74.
16. F. Kohlmeier, P.M. Schneider, Successful mRNA profiling of 23 years old blood stains, Forensic Sci. Int. Genet. (2011), doi:10.1016/j.fsigen.2011.04. 007.
17. M. Kemp, K.H. Jensen, R. Dargis, J.J. Christensen, Routine ribosomal PCR and DNA sequencing for detection and identification of bacteria, Future Microbiol. 5 (2010) 1101-1107.
18. E. Frahm, U. Obst, Application of the fluorogenic probe technique (TaqMan PCR) to the detection of Enterococcus spp. and Escherichia coli in water samples, J. Microbiol. Methods 52 (2003) 123-131. (Pubitemid 35238057)
19. W.E. Kloos, M.S. Musselwhite, Distribution and persistence of Staphylococcus and Micrococcus species and other aerobic bacteria on human skin, Appl. Microbiol. 30 (1975) 381-385.
20. N. Fierer, C.L. Lauber, N. Zhou, D. McDonald, E.K. Costello, R. Knight, Forensic identification using skin bacterial communities, PNAS 107 (2010) 6477-6481.
21. K. Kupferschmidt, Epidemiology. Outbreak detectives embrace the genome era, Science 333 (2011) 1818-1819.
22. R.I. Fleming, S.A. Harbison, The use of bacteria for the identification of vaginal secretions, J. Forensic Sci. 4 (2010) 311-315.
23. H. Nakanishi, A. Kido, T. Ohmori, A. Takada, M. Hara, N. Adachi, K. Saito, A novel method for the identification of saliva by detecting oral Streptococci using PCR, Forensic Sci. Int. 183 (2009) 20-23.
24. H. Nakanishi, T. Ohmori, M. Hara, A. Takada, H. Shojo, N. Adachi, K. Saito, A simple identification method of saliva by detecting Streptococcus salivarius using loop-mediated isothermal amplification, J. Forensic Sci. 56 (2011) S158-S161.
25. J. Brosius, T.J. Dull, D.D. Sleeter, H.F. Noller, Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli, J. Mol. Biol. 148 (1981) 107-127. (Pubitemid 11084759)
26. S. Juretschko, G. Timmermann, M. Schmid, K.H. Schleifer, A. Pommerening-Röser, H.P. Koops, M. Wagner, Combined molecular and conventional analyses of nitrifying bacterium diversity in activated sludge: Nitrosococcus mobilis and Nitrospira-like bacteria as dominant populations, Appl. Environ. Microbiol. 64 (1998) 3042-3051. (Pubitemid 28363182)
27. S. Rozen, H.J. Skaletsky, Primer3 on the WWWfor general users and for biologist programmers, in: S. Krawetz, S. Misener (Eds.), Bioinformatics Methods and Protocols: Methods in Molecular Biology, Humana Press, Totowa, NJ, 2000, pp. 365-386.
28. F. Barni, A. Berti, A. Pianese, A. Boccellino, M.P. Miller, A. Caperna, G. Lago, Allele frequencies of 15 autosomal STR loci in the Iraq population with comparisons to other populations from the middle-eastern region, Forensic Sci. Int. 167 (2007) 87-92. (Pubitemid 46420633)
29. J. Ravel, P. Gajer, Z. Abdo, G.M. Schneider, S.S. Koenig, S.L. McCulle, S. Karlebach, R. Gorle, J. Russell, C.O. Tacket, R.M. Brotman, C.C. Davis, K. Ault, L. Peralta, L.J. Forney, Vaginal microbiome of reproductive-age women, PNAS 108 (2011) 4680-4687.
30. Association of Forensic Science Providers, Standards for the formulation of evaluative forensic science expert opinion, Sci. Justice 49 (2009) 161-164.
31. N.A. El Aila, I. Tency, G. Claeys, H. Verstraelen, B. Saerens, G.L. Santiago, E. De Backer, P. Cools, M. Temmerman, R. Verhelst, M. Vaneechoutte, Identification and genotyping of bacteria from paired vaginal and rectal samples from pregnant women indicates similarity between vaginal and rectal microflora, BMC Infect. Dis. 9 (2009) 167.
32. L. Deng, X. Pan, Y. Wang, L. Wang, X.E. Zhou, M. Li, Y. Feng, Q. Wu, B. Wang, N. Huang, Hemoglobin and its derived peptides may play a role in the antibacterial mechanism of the vagina, Hum. Reprod. 24 (2009) 211-218.
33. S. Srinivasan, C. Liu, C.M. Mitchell, T.L. Fiedler, K.K. Thomas, K.J. Agnew, J.M. Marrazzo, D.N. Fredricks, Temporal variability of human vaginal bacteria and relationship with bacterial vaginosis, PLoS ONE 5 (2010) e10197.