Quantification of bacterial species of the vaginal microbiome in different groups of women, using nucleic acid amplification tests

BMC Microbiology

Volumn 12, Issue , 2012, Pages

  Jespers, Vicky ^a   Menten, Joris ^a   Smet, Hilde ^a   Poradosú, Sabrina ^a   Abdellati, Sad ^a   Verhelst, Rita ^b   Hardy, Liselotte ^a   Buvé, Anne ^a   Crucitti, Tania ^a  

^a INSTITUTE OF TROPICAL MEDICINE   (Belgium)

^b GHENT UNIVERSITY   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; AFRICAN AMERICAN; ARTICLE; ASIAN; ATOPOBIUM VAGINAE; BACTERIUM; CAUCASIAN; CLINICAL ARTICLE; CONTROLLED STUDY; ETHNICITY; FEMALE; GARDNERELLA VAGINALIS; HUMAN; LACTOBACILLUS; LACTOBACILLUS CRISPATUS; LACTOBACILLUS GASSERI; LACTOBACILLUS INERS; LACTOBACILLUS JENSENII; MENSTRUAL CYCLE; MICROBIOME; NONHUMAN; NUCLEIC ACID AMPLIFICATION; POLYMERASE CHAIN REACTION; VAGINA;

ADOLESCENT; ADULT; BACTERIA; BACTERIAL LOAD; BIODIVERSITY; ETHNIC GROUPS; FEMALE; HUMANS; METAGENOME; NUCLEIC ACID AMPLIFICATION TECHNIQUES; VAGINA; YOUNG ADULT;

BACTERIA (MICROORGANISMS); LACTOBACILLUS; LACTOBACILLUS CRISPATUS; LACTOBACILLUS GASSERI; LACTOBACILLUS INERS; LACTOBACILLUS JENSENII; LACTOBACILLUS VAGINALIS;

EID: 84861556010     PISSN: None     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-12-83     Document Type: Article

References (31)

1. Intravaginal practices, bacterial vaginosis, and womens susceptibility to HIV infection: epidemiological evidence and biological mechanisms. Myer L, Kuhn L, Stein ZA, Wright TC, Denny L, Lancet Infect Dis 2005 5 786 794 10.1016/S1473-3099(05)70298-X 16310150
2. Bacterial vaginosis and disturbances of vaginal flora: association with increased acquisition of HIV. Taha TE, Hoover DR, Dallabetta GA, Kumwenda NI, Mtimavalye LA, Yang LP, Liomba GN, Broadhead RL, Chiphangwi JD, Miotti PG, AIDS 1998 12 1699 1706 10.1097/00002030-199813000-00019 9764791
3. Disentangling contributions of reproductive tract infections to HIV acquisition in African Women. van de Wijgert JH, Morrison CS, Brown J, Kwok C, Van Der PB, Chipato T, Byamugisha JK, Padian N, Salata RA, Sex Transm Dis 2009 36 357 364 10.1097/OLQ.0b013e3181a4f695 19434010
4. The effects of commensal bacteria on innate immune responses in the female genital tract. Mirmonsef P, Gilbert D, Zariffard MR, Hamaker BR, Kaur A, Landay AL, Spear GT, Am J Reprod Immunol 2011 65 190 195 10.1111/j.1600-0897. 2010.00943.x 21143335
5. The normal vaginal flora, H2O2-producing lactobacilli, and bacterial vaginosis in pregnant women. Hillier SL, Krohn MA, Rabe LK, Klebanoff SJ, Eschenbach DA, Clin Infect Dis 1993 16 Suppl 4 273 S281 8324131
6. Viricidal effect of Lactobacillus acidophilus on human immunodeficiency virus type 1: possible role in heterosexual transmission. Klebanoff SJ, Coombs RW, J Exp Med 1991 174 289 292 10.1084/jem.174.1.289 1647436
7. A delicate balance: risk factors for acquisition of bacterial vaginosis include sexual activity, absence of hydrogen peroxide-producing lactobacilli, black race, and positive herpes simplex virus type 2 serology. Cherpes TL, Hillier SL, Meyn LA, Busch JL, Krohn MA, Sex Transm Dis 2008 35 78 83 10.1097/OLQ.0b013e318156a5d0 17989585
8. Reliability of diagnosing bacterial vaginosis is improved by a standardized method of gram stain interpretation. Nugent R, Krohn M, Hillier S, J Clin Microbiol 1991 29 297 301 1706728
9. Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity. Hugenholtz P, Goebel BM, Pace NR, J Bacteriol 1998 180 4765 4774 9733676
10. Utility of Amsel criteria, Nugent score, and quantitative PCR for Gardnerella vaginalis, Mycoplasma hominis, and Lactobacillus spp. for diagnosis of bacterial vaginosis in human immunodeficiency virus-infected women. Sha BE, Chen HY, Wang QJ, Zariffard MR, Cohen MH, Spear GT, J Clin Microbiol 2005 43 4607 4612 10.1128/JCM.43.9.4607-4612.2005 16145114
11. Cloning of 16S rRNA genes amplified from normal and disturbed vaginal microflora suggests a strong association between Atopobium vaginae, Gardnerella vaginalis and bacterial vaginosis. Verhelst R, Verstraelen H, Claeys G, Verschraegen G, Delanghe J, Van Simaey L, De Ganck C, Temmerman M, Vaneechoutte M, BMC Microbiol 2004 4 16 10.1186/1471-2180-4-16 15102329
12. Targeted PCR for detection of vaginal bacteria associated with bacterial vaginosis. Fredricks DN, Fiedler TL, Thomas KK, Oakley BB, Marrazzo JM, J Clin Microbiol 2007 45 3270 3276 10.1128/JCM.01272-07 17687006
13. Deep sequencing of the vaginal microbiota of women with HIV. Hummelen R, Fernandes AD, Macklaim JM, Dickson RJ, Changalucha J, Gloor GB, Reid G, PLoS One 2010 5 12078 10.1371/journal.pone.0012078 20711427
14. Vaginal microbiome of reproductive-age women. Ravel J, Gajer P, Abdo Z, Schneider GM, Koenig SS, McCulle SL, Karlebach S, Gorle R, Russell J, Tacket CO, Brotman RM, Davis CC, Ault K, Peralta L, Forney LJ, Proc Natl Acad Sci USA 2011 108 Suppl 1 4680 4687 20534435
15. Pyrosequencing of the genital microbiotas of HIV-seropositive and -seronegative women reveals Lactobacillus iners as the predominant Lactobacillus Species. Spear GT, Gilbert D, Landay AL, Zariffard R, French AL, Patel P, Gillevet PM, Appl Environ Microbiol 2011 77 378 381 10.1128/AEM.00973-10 21075899
16. Differences in the composition of vaginal microbial communities found in healthy Caucasian and black women. Zhou X, Brown CJ, Abdo Z, Davis CC, Hansmann MA, Joyce P, Foster JA, Forney LJ, ISME J 2007 1 121 133 10.1038/ismej.2007.12 18043622
17. The vaginal microbiome: new information about genital tract flora using molecular based techniques. Lamont R, Sobel J, Akins R, Hassan S, Chaiworapongsa T, Kusanovic J, Romero R, BJOG 2011 118 533 549 10.1111/j.1471-0528.2010.02840. x 21251190
18. Temporal variability of human vaginal bacteria and relationship with bacterial vaginosis. Srinivasan S, Liu C, Mitchell CM, Fiedler TL, Thomas KK, Agnew KJ, Marrazzo JM, Fredricks DN, PLoS One 2010 5 10197 10.1371/journal.pone. 0010197 20419168
19. Longitudinal analysis of the vaginal microflora in pregnancy suggests that L. crispatus promotes the stability of the normal vaginal microflora and that L. gasseri and/or L. iners are more conducive to the occurrence of abnormal vaginal microflora. Verstraelen H, Verhelst R, Claeys G, De Backer E, Temmerman M, Vaneechoutte M, BMC Microbiol 2009 9 116 10.1186/1471-2180-9-116 19490622
20. Longitudinal study of the dynamics of vaginal microflora during two consecutive menstrual cycles. Santiago GL, Cools P, Verstraelen H, Trog M, Missine G, El Aila N, Verhelst R, Tency I, Claeys G, Temmerman M, Vaneechoutte M, PLoS One 2011 6 28180 10.1371/journal.pone.0028180 22140538
21. Dose-ranging phase 1 study of TMC120, a promising vaginal microbicide, in HIV-negative and HIV-positive female volunteers. Jespers VA, Van Roey JM, Beets GI, Buve AM, J Acquir Immune Defic Syndr 2007 44 154 158 10.1097/QAI. 0b013e31802bb35f 17106275
22. McCutcheon AL, Latent Class Analysis. Quantitative Applications in the Social Sciences Series N° 64 Sage Publication, Thousand Oaks 1987 edition
23. Extended antimicrobial treatment of bacterial vaginosis combined with human lactobacilli to find the best treatment and minimize the risk of relapses. Larsson PG, Brandsborg E, Forsum U, Pendharkar S, Krogh-Andersen K, Nasic S, Hammarstrom L, Marcotte H, BMC Infect Dis 2011 11 223 10.1186/1471-2334-11-223 21854593
24. Molecular quantification of Gardnerella vaginalis and Atopobium vaginae loads to predict bacterial vaginosis. Menard JP, Fenollar F, Henry M, Bretelle F, Raoult D, Clin Infect Dis 2008 47 33 43 10.1086/588661 18513147
25. The prevalence and incidence of bacterial vaginosis in a cohort of young Australian women. Walker J, Hocking J, Fairley C, Tabrizi S, Chen M, Bowden F, Gunn J, Donovan B, Kaldor J, Bradshaw C, Sex Transm Infect 2011 Vol 87 uppl 1
26. The vaginal bacterial communities of Japanese women resemble those of women in other racial groups. Zhou X, Hansmann MA, Davis CC, Suzuki H, Brown CJ, Schutte U, Pierson JD, Forney LJ, FEMS Immunol Med Microbiol 2010 58 169 181 10.1111/j.1574-695X.2009.00618.x 19912342
27. Lactobacillus crispatus colonisation reduces risk of bacterial vaginosis (BV) acquisition. Antonio M, Petrina M, Meyn L, Hillier S, Sex Transm Dis 2011 Vol 87 Suppl 1 304 A305
28. Detection of bacterial vaginosis-related organisms by real-time PCR for Lactobacilli, Gardnerella vaginalis and Mycoplasma hominis. Zariffard MR, Saifuddin M, Sha BE, Spear GT, FEMS Immunol Med Microbiol 2002 34 277 281 10.1111/j.1574-695X.2002.tb00634.x 12443827
29. Quantitative analysis of diverse Lactobacillus species present in advanced dental caries. Byun R, Nadkarni MA, Chhour KL, Martin FE, Jacques NA, Hunter N, J Clin Microbiol 2004 42 3128 3136 10.1128/JCM.42.7.3128-3136.2004 15243071
30. Association between Lactobacillus species and bacterial vaginosis-related bacteria, and bacterial vaginosis scores in pregnant Japanese women. Tamrakar R, Yamada T, Furuta I, Cho K, Morikawa M, Yamada H, Sakuragi N, Minakami H, BMC Infect Dis 2007 7 128 10.1186/1471-2334-7-128 17986357
31. Quantitative determination by real-time PCR of four vaginal Lactobacillus species. Gardnerella vaginalis and Atopobium vaginae indicates an inverse relationship between L. gasseri and L. iners. De Backer E, Verhelst R, Verstraelen H, Alqumber MA, Burton JP, Tagg JR, Temmerman M, Vaneechoutte M, BMC Microbiol 2007 7 115 10.1186/1471-2180-7-115 18093311