High-level expression and purification of recombinant human growth hormone produced in soluble form in Escherichia coli

Protein Expression and Purification

Volumn 100, Issue , 2014, Pages 40-47

  Levarski, Zdenko ^a   Šoltýsová, Andrea ^a   Krahulec, Ján ^a   Stuchlík, Stanislav ^a   Turňa, Ján ^a  

^a Faculty of Natural Sciences   (Slovakia)

Author keywords

Escherichia coli; Human growth hormone; Nb2 assay; Recombinant protein

Indexed keywords

HUMAN GROWTH HORMONE; RECOMBINANT PROTEIN; THIOREDOXIN;

AFFINITY CHROMATOGRAPHY; BACTERIUM TRANSFORMATION; CELL LINE; CELL PROLIFERATION; CHEMISTRY; DRUG EFFECTS; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENETICS; HUMAN; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; MOLECULAR CLONING; PROCEDURES; SOLUBILITY;

CELL LINE; CELL PROLIFERATION; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; CLONING, MOLECULAR; ESCHERICHIA COLI; HUMAN GROWTH HORMONE; HUMANS; RECOMBINANT PROTEINS; SOLUBILITY; THIOREDOXINS; TRANSFORMATION, BACTERIAL;

EID: 84901928431     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2014.05.003     Document Type: Article

References (45)

1. A.M. de Vos, M. Ultsch, and A.A. Kossiakoff Human growth hormone and extracellular domain of its receptor: crystal structure of the complex Science 255 1992 306 312
2. M.H. Ultsch, W. Somers, A.A. Kossiakoff, and A.M. Devos The crystal-structure of affinity-matured human growth-hormone at 2-Angstrom resolution J. Mol. Biol. 236 1994 286 299
3. G.P. Baumann Growth hormone isoforms Growth Horm. IGF Res. 19 2009 333 340
4. J. Lindholm Growth hormone: historical notes Pituitary 9 2006 5 10 (Pubitemid 43733077)
5. H.P. Sorensen, and K.K. Mortensen Advanced genetic strategies for recombinant protein expression in Escherichia coli J. Biotechnol. 115 2005 113 128 (Pubitemid 39656438)
6. M.M. Carrio, and A. Villaverde Construction and deconstruction of bacterial inclusion bodies J. Biotechnol. 96 2002 3 12 (Pubitemid 34876295)
7. D.V. Goeddel, H.L. Heyneker, T. Hozumi, R. Arentzen, K. Itakura, D.G. Yansura, M.J. Ross, G. Miozzari, R. Crea, and P.H. Seeburg Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone Nature 281 1979 544 548 (Pubitemid 10242863)
8. D.S. Waugh Making the most of affinity tags Trends Biotechnol. 23 2005 316 320 (Pubitemid 40726273)
9. R. Mukhija, P. Rupa, D. Pillai, and L.C. Garg High level production and one step purification of biologically active human growth hormone in Escherichia coli Gene 165 1995 303 306
10. A.K. Patra, R. Mukhopadhyay, R. Mukhija, A. Krishnan, L.C. Garg, and A.K. Panda Optimization of inclusion body solubilization and renaturation of recombinant human growth hormone from Escherichia coli Protein Expr. Purif. 18 2000 182 192 (Pubitemid 30172224)
11. H. Schellekens, and N. Casadevall Immunogenicity of recombinant human proteins: causes and consequences J. Neurol. 251 Suppl. 2 2004 II4 II9
12. C.S. Kim, and E.K. Lee Effects of operating parameters in in vitro renaturation of a fusion protein of human growth hormone and glutathione S transferase from inclusion body Process Biochem. 36 2000 111 117
13. N.K. Shin, D.Y. Kim, C.S. Shin, M.S. Hong, J. Lee, and H.C. Shin High-level production of human growth hormone in Escherichia coli by a simple recombinant process J. Biotechnol. 62 1998 143 151 (Pubitemid 28321505)
14. H. Dalbøge, H.-H.M. Dahl, J. Pedersen, J.W. Hansen, and T. Christensen A novel enzymatic method for production of authentic hGH from an Escherichia coli produced hGH-precursor Nat. Biotechnol. 5 1987 161 164 (Pubitemid 17009140)
15. T.Y. Koo, and T.H. Park Expression of recombinant human growth hormone in a soluble form in Escherichia coli by slowing down the protein synthesis rate J. Microbiol. Biotechnol. 17 2007 579 585 (Pubitemid 46739120)
16. S. Roytrakul, L. Eurwilaichitr, C. Suprasongsin, and S. Panyim A rapid and simple method for construction and expression of a synthetic human growth hormone gene in Escherichia coli J. Biochem. Mol. Biol. 34 2001 502 508
17. M.J. Kim, H.S. Park, K.H. Seo, H.J. Yang, S.K. Kim, and J.H. Choi Complete solubilization and purification of recombinant human growth hormone produced in Escherichia coli PLoS One 8 2013 e56168
18. M.T. Nguyen, B.K. Koo, T.T. Thi Vu, J.A. Song, S.H. Chong, B. Jeong, H.B. Ryu, S.H. Moh, and H. Choe Prokaryotic soluble overexpression and purification of bioactive human growth hormone by fusion to thioredoxin, maltose binding protein, and protein disulfide isomerase PLoS One 9 2014 e89038
19. H. Uchida, N. Naito, N. Asada, M. Wada, M. Ikeda, H. Kobayashi, M. Asanagi, K. Mori, Y. Fujita, K. Konda, N. Kusuhara, T. Kamioka, K. Nakashima, and M. Honjo Secretion of authentic 20-kDa human growth hormone (20K hGH) in Escherichia coli and properties of the purified product J. Biotechnol. 55 1997 101 112 (Pubitemid 27376548)
20. J.T. Sockolosky, and F.C. Szoka Periplasmic production via the pET expression system of soluble, bioactive human growth hormone Protein Expr. Purif. 87 2013 129 135
21. C. Kato, T. Kobayashi, T. Kudo, T. Furusato, Y. Murakami, T. Tanaka, H. Baba, T. Oishi, E. Ohtsuka, M. Ikehara, T. Yanagida, H. Kato, S. Moriyama, and K. Horikoshi Construction of an excretion vector and extracellular production of human growth hormone from Escherichia coli Gene 54 1987 197 202 (Pubitemid 17100371)
22. T. Kajino, Y. Saito, O. Asami, Y. Yamada, M. Hirai, and S. Udata Extracellular production of an intact and biologically active human growth hormone by the Bacillus brevis system J. Ind. Microbiol. Biotechnol. 19 1997 227 231 (Pubitemid 28032080)
23. T.H. Ozdamar, B. Senturk, O.D. Yilmaz, G. Calik, E. Celik, and P. Calik Expression system for recombinant human growth hormone production from Bacillus subtilis Biotechnol. Prog. 25 2009 75 84
24. T. Tokunaga, S. Iwai, H. Gomi, K. Kodama, E. Ohtsuka, M. Ikehara, O. Chisaka, and K. Matsubara Expression of a synthetic human growth hormone gene in yeast Gene 39 1985 117 120 (Pubitemid 16177086)
25. M.S. Hahm, and B.H. Chung Secretory expression of human growth hormone in Saccharomyces cerevisiae using three different leader sequences Biotechnol. Bioprocess Eng. 6 2001 306 309 (Pubitemid 33811619)
26. L.L. Ecamilla-Trevino, J.M. Viader-Salvado, H.A. Barrera-Saldana, and M. Guerrero-Olazaran Biosynthesis and secretion of recombinant human growth hormone in Pichia pastoris Biotechnol. Lett. 22 2000 109 114 (Pubitemid 30084556)
27. P. Calik, M.A. Orman, E. Celik, S.M. Halloran, G. Calik, and T.H. Ozdamar Expression system for synthesis and purification of recombinant human growth hormone in Pichia pastoris and structural analysis by MALDI-ToF Mass Spectrometry Biotechnol. Prog. 24 2008 221 226 (Pubitemid 351231271)
28. K. Kadonookuda, M. Yamamoto, Y. Higashino, K. Taniai, Y. Kato, S. Chowdhury, J.H. Xu, S.K. Choi, M. Sugiyama, K. Nakashima, S. Maeda, and M. Yamakawa Baculovirus mediated production of the human growth hormone in larvae of the silkworm, Bombyx mori Biochem. Biophys. Res. Commun. 213 1995 389 396
29. H. Lan, Z. Nie, Y. Liu, Z. Lv, Y. Liu, Y. Quan, J. Chen, Q. Zhen, Q. Chen, D. Wang, Q. Sheng, W. Yu, J. Chen, X. Wu, and Y. Zhang In vivo bioassay of recombinant human growth hormone synthesized in B. mori pupae J. Biomed. Biotechnol. 2010 2010 306462
30. J.H. Lupker, W.G. Roskam, B. Miloux, P. Liauzun, M. Yaniv, and J. Jouannau Abundant excretion of human growth hormone by recombinant plasmid transformed monkey kidney cells Gene 24 1983 281 287 (Pubitemid 13018262)
31. D. Catzel, H. Lalevski, C.P. Marquis, P.P. Gray, D. Van Dyk, and S.M. Mahler Purification of recombinant human growth hormone from CHO cell culture supernatant by Gradiflow preparative electrophoresis technology Protein Expr. Purif. 32 2003 126 134 (Pubitemid 37436146)
32. J. Xu, S. Okada, L. Tan, K.J. Goodrum, J.J. Kopchick, and M.J. Kieliszewski Human growth hormone expressed in tobacco cells as an arabinogalactan-protein fusion glycoprotein has a prolonged serum life Transgenic Res. 19 2010 849 867
33. B. Llompart, I. Llop-Tous, P. Marzabal, M. Torrent, R. Pallissé, M. Bastida, M.D. Ludevid, and F. Walas Protein production from recombinant protein bodies Process Biochem. 45 2010 1816 1820
34. T. Shigemitsu, S. Ozaki, Y. Saito, M. Kuroda, S. Morita, S. Satoh, and T. Masumura Production of human growth hormone in transgenic rice seeds: co-introduction of RNA interference cassette for suppressing the gene expression of endogenous storage proteins Plant Cell Rep. 31 2012 539 549
35. J.S. Archer, W.S. Kennan, M.N. Gould, and R.D. Bremel Human growth-hormone (Hgh) secretion in milk of goats after direct transfer of the Hgh gene into the mammary-gland by using replication-defective retrovirus vectors Proc. Natl. Acad. Sci. U.S.A. 91 1994 6840 6844
36. J.R. Hens, M.D. Amstutz, F.L. Schanbacher, and I.H. Mather Introduction of the human growth hormone gene into the guinea pig mammary gland by in vivo transfection promotes sustained expression of human growth hormone in the milk throughout lactation Biochim. Biophys. Acta 1523 2000 161 171
37. D. Salamone, L. Baranao, C. Santos, L. Bussmann, J. Artuso, C. Werning, A. Prync, C. Carbonetto, S. Dabsys, C. Munar, R. Salaberry, G. Berra, I. Berra, N. Fernandez, M. Papouchado, M. Foti, N. Judewicz, I. Mujica, L. Munoz, S.F. Alvarez, E. Gonzalez, J. Zimmermann, M. Criscuolo, and C. Melo High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow J. Biotechnol. 124 2006 469 472 (Pubitemid 43959825)
38. J. Krahulec, M. Hyrsova, S. Pepeliaev, J. Jilkova, Z. Cerny, and J. Machalkova High level expression and purification of antimicrobial human cathelicidin LL-37 in Escherichia coli Appl. Microbiol. Biotechnol. 88 2010 167 175
39. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
40. Y. Aida, and M.J. Pabst Removal of endotoxin from protein solutions by phase separation using Triton X-114 J. Immunol. Methods 132 1990 191 195 (Pubitemid 20281877)
41. M.E. Gasparian, M.L. Bychkov, D.A. Dolgikh, and M.P. Kirpichnikov Strategy for improvement of enteropeptidase efficiency in tag removal processes Protein Expr. Purif. 79 2011 191 196
42. E.R. LaVallie, E.A. DiBlasio, S. Kovacic, K.L. Grant, P.F. Schendel, and J.M. McCoy A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm Nat. Biotechnol. 11 1993 187 193 (Pubitemid 23056045)
43. M.E. Gasparian, V.G. Ostapchenko, D.A. Dolgikh, and M.P. Kirpichnikov Biochemical characterization of human enteropeptidase light chain Biochemistry (Moscow) 71 2006 113 119
44. H.C. Birnboim, and J. Doly A rapid alkaline extraction procedure for screening recombinant plasmid DNA Nucleic Acids Res. 7 1979 1513 1523
45. P. Malyala, and M. Singh Endotoxin limits in formulations for preclinical research J. Pharm. Sci. 97 2008 2041 2044