Secretory expression of human growth hormone in Saccharomyces cerevisiae using three different leader sequences

Biotechnology and Bioprocess Engineering

Volumn 6, Issue 4, 2001, Pages 306-309

  Hahm, Moon Sun ^a   Chung, Bong Hyun ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

Human growth hormone; Leader sequence; Saccharomyces cerevisiae; Secretion

Indexed keywords

EID: 46849115458     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02931995     Document Type: Article

References (18)

1. Romanos, M. A., C. A. Scorer, and J. J. Clare (1992) Foreign gene expression in yeast: A review. Yeast 8: 423-488.
2. Martial, J. A., R. A. Hallewell, J. D. Baxter, and H. M. Goodman (1979) Human growth hormone: complementary DNA cloning and expression in bacteria. Science 205: 602-607.
3. Goeddel, D. V., H. L. Heyneker, T. Hozumi, R. Arentzen, K. Itakura, D. G. Yansura, G. Miozzari, R. Crea, and P. H. Seeburg (1979) Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone. Nature 281: 544-548.
4. Chang, N. C., M. Rey B. Bochner, H. Heynecker, and G. Grey (1987) High-level secretion of human growth hormone by Escherichia coli. Gene 55: 189-196.
5. Dalboge, H, S. Carlsen, E. B. Jensen, T. Christensen, and H. M. Dahl (1988) Expression of recombinant growth hormone in Escherichia coli: Effect of the region between the Shine-Dalgarno sequence and the ATG initiation codon. DNA 7: 399-405.
6. Gellerfors, P., G. Eketorp, K. Fhulenhag, B. Pavlu, S. Johansson, and L. Fryklund (1989) Characterization of a secreted form of recombinant derived human growth hormone in Escherichia coli cells. J. Pharm. Biomed. Anal. 7: 173-183.
7. K. Glasbrenner (1986) Technology spurt resolves growth hormone problem, ends shortage. J. Am. Med. Assoc. 255: 581-587.
8. Gray, G. L., K. A. McKeown, A. J. S. Jones, P. H. Seeburg, and H. L. Heynecker (1984) Pseudomonas aeruginosa secretes and correctly processes human growth hormone. Biotechnology 2: 161-165.
9. Gray, G. L., J. S. Baldridge, K. S. McKeown, H. L. Heyneker, and C N. Chang (1985) Periplasmic production of correctly processed human growth hormone in Escherichia coli: Natural and bacterial signal sequences are interchangeable. Gene 39: 247-254.
10. Nakayama, A., K. Kawamura, H. Shimada, A. Akaoka, J. Mita, M. Honjo, and Y. Furutani (1987) Extracellular production of human growth hormone by a head portion of the prepropeptide derived from Bacillus amyloliquefaciens neutral protease in Bacillus subtilis. J. Biotechnol. 5: 171-179.
11. Shin, N. K., D. Y. Kim, C. S. Shin, M. S. Hong, J. Lee and H. C. Shin (1998) High-level production of human growth hormone in Escherichia coli by a simple recombinant process. J. Biotechnol. 62: 143-151.
12. Chung, B. H., D. J. Seo, and S. W. Nam (1999) High-level secretory production of recombinant human lipocortin-I by Saccharomyces cerevisiae. Process Biochem. 35: 97-101.
13. Ito, H., Y. Fukuda, K. Murata, and A. Kimura (1983) Transformation of intact yeast cells with alkali cations. J. Bacteriol. 153: 163-168.
14. Chung, B. H., S. W. Nam, B. M. Kim, and Y. H. Park (1996) Highly efficient secretion of heterologous proteins from Saccharomyces cerevisiae using inulinase signal peptides. Biotechnol. Bioeng. 49: 473-479.
15. s1-casein in Saccharomyces cerevisiae. J. Microbiol. Biotechnol. 9: 196-200.
16. Hitzeman, R. A., D. W. Lenny, L. J. Petty, W. J. Kohr, H. L. Levine, and D. V. Goeddel (1983) Secretion of human interferons by yeast. Science 219: 620-625.
17. 1-antitrypsin cDNA in the yeast Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 81:6594-6598.
18. Haguenauer-Tsapis, R. and A. Hinnen (1984) A deletion that includes the signal peptidase cleavage site impairs processing, glycosylation, and secretion of cell surface yeast acid phosphatase. Mol. Cell. Biol. 4: 2668-2675.