Secretion of authentic 20-kDa human growth hormone (20K hGH) in Escherichia coli and properties of the purified product

Journal of Biotechnology

Volumn 55, Issue 2, 1997, Pages 101-112

  Uchida, H ^a   Naito, N ^a   Asada, N ^a   Wada, M ^a   Ikeda, M ^a   Kobayashi, H ^a   Asanagi, M ^a   Mori, K ^a   Fujita, Y ^a   Konda, K ^a   Kusuhara, N ^a   Kamioka, T ^b   Nakashima, K ^c   Honjo, M ^a  

^a MITSUI CHEMICALS INC   (Japan)

^b Mitsui Pharmaceuticals Inc   (Japan)

^c MIE UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

20K hGH; Authentic structure; Biological activities; E. coli; Glutathione reductase; Secretion

Indexed keywords

BODY FLUIDS; CELL CULTURE; CHEMICAL MODIFICATION; ENZYME KINETICS; HORMONES; MICROORGANISMS; PROTEINS;

BACILLUS AMYLOLIQUEFACIENS; GLUTATHIONE REDUCTASE; HUMAN GROWTH HORMONE; LIPOLYSIS STIMULATION; PHYSICOCHEMICAL PROPERTIES;

GROWTH KINETICS;

GLUTATHIONE REDUCTASE; HUMAN GROWTH HORMONE;

ALTERNATIVE RNA SPLICING; ARTICLE; CELL MEMBRANE; CELL PROLIFERATION; DISULFIDE BOND; ESCHERICHIA COLI; GENETIC RECOMBINATION; HORMONE RELEASE; LIPOLYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN PURIFICATION; RECOMBINANT DNA TECHNOLOGY;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; DNA, RECOMBINANT; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HUMAN GROWTH HORMONE; HUMANS; MOLECULAR SEQUENCE DATA; RATS; RECOMBINANT PROTEINS;

BACILLUS AMYLOLIQUEFACIENS; ESCHERICHIA COLI;

EID: 0343811713     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(97)00062-X     Document Type: Article

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