Droplet Array Platform for High-Resolution Melt Analysis of DNA Methylation Density

Journal of Laboratory Automation

Volumn 19, Issue 3, 2014, Pages 304-312

  Athamanolap, Pornpat ^a   Shin, Dong Jin ^a   Wang, Tza Huei ^b  

^a Johns Hopkins University   (United States)

^b Johns Hopkins University   (United States)

Author keywords

DNA methylation detection; melt curve analysis; miniaturized system

Indexed keywords

ALKYLATION; DROP FORMATION; MELTING; METHYLATION;

CURVE ANALYSIS; DNA METHYLATION; DROPLET PLATFORMS; METHYLATION-SPECIFIC PCR; MICROFLUIDIC DROPLETS; MINIATURIZED SYSTEMS; POINT-OF-CARE DIAGNOSTICS; SPECIFIC INFORMATION;

POLYMERASE CHAIN REACTION;

BISULFITE; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; HYDROGEN SULFITE; RASSF1 PROTEIN, HUMAN; RECOMBINANT DNA; SULFITE; TUMOR SUPPRESSOR PROTEIN;

ANALYTIC METHOD; ARTICLE; DNA METHYLATION; DNA SEQUENCE; HIGH RESOLUTION MELTING ANALYSIS; MEDICAL DEVICE; MELTING POINT; MICROFLUIDIC DEVICE; POLYMERASE CHAIN REACTION; SIGNAL PROCESSING; THERMAL CONDUCTIVITY; CALIBRATION; CHEMISTRY; COMPARATIVE STUDY; CPG ISLAND; DNA DENATURATION; GENETICS; HUMAN; LABORATORY AUTOMATION; METABOLISM; MICROARRAY ANALYSIS; MICROFLUIDIC ANALYSIS; MINIATURIZATION; POINT OF CARE TESTING; PROMOTER REGION; PROOF OF CONCEPT; REPRODUCIBILITY; TRANSITION TEMPERATURE;

AUTOMATION, LABORATORY; CALIBRATION; CPG ISLANDS; DNA METHYLATION; DNA, RECOMBINANT; HUMANS; INDICATORS AND REAGENTS; MICROARRAY ANALYSIS; MICROFLUIDIC ANALYTICAL TECHNIQUES; MINIATURIZATION; NUCLEIC ACID DENATURATION; POINT-OF-CARE TESTING; POLYMERASE CHAIN REACTION; PROMOTER REGIONS, GENETIC; PROOF OF CONCEPT STUDY; REPRODUCIBILITY OF RESULTS; SULFITES; TRANSITION TEMPERATURE; TUMOR SUPPRESSOR PROTEINS;

EID: 84901034964     PISSN: 22110682     EISSN: 22110690     Source Type: Journal    
DOI: 10.1177/2211068213507923     Document Type: Article

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