Validation of methylation-sensitive high resolution melting for the detection of DNA methylation in cholangiocarcinoma

Clinical Biochemistry

Volumn 45, Issue 13-14, 2012, Pages 1092-1094

  Amornpisutt, Rattaya ^a   Sriraksa, Ruethairat ^a,b   Limpaiboon, Temduang ^a,b  

^a KHON KAEN UNIVERSITY   (Thailand)

^b KHON KAEN UNIVERSITY   (Thailand)

Author keywords

DNA methylation; Epigenetic; Methylation sensitive high resolution melting; Methylation specific PCR; Pyrosequencing

Indexed keywords

CELL ADHESION MOLECULE; DNA; OPIATE BINDING PROTEIN; TUMOR NECROSIS FACTOR RELATED APOPTOSIS INDUCING LIGAND RECEPTOR 3; UNCLASSIFIED DRUG;

ARTICLE; BILE DUCT CARCINOMA; CONTROLLED STUDY; DNA METHYLATION; HIGH RESOLUTION COMPUTER TOMOGRAPHY; HIGH RESOLUTION MELTING ANALYSIS; HUMAN; HUMAN CELL; HUMAN TISSUE; METHYLATION SENSITIVE HIGH RESOLUTION MELTING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PYROSEQUENCING; RELIABILITY; VALIDATION PROCESS;

BILE DUCT NEOPLASMS; BILE DUCTS, INTRAHEPATIC; CELL ADHESION MOLECULES; CHOLANGIOCARCINOMA; CPG ISLANDS; DNA METHYLATION; GENETIC LOCI; GENOME, HUMAN; GPI-LINKED PROTEINS; HUMANS; LINEAR MODELS; NUCLEIC ACID DENATURATION; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; TUMOR NECROSIS FACTOR DECOY RECEPTORS;

EID: 84866005688     PISSN: 00099120     EISSN: 18732933     Source Type: Journal    
DOI: 10.1016/j.clinbiochem.2012.04.027     Document Type: Article

References (10)

1. Herman J.G., Graff J.R., Myohanen S., Nelkin B.D., Baylin S.B. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci U S A 1996, 93:9821-9826.
2. Kristensen L.S., Hansen L.L. PCR-based methods for detecting single-locus DNA methylation biomarkers in cancer diagnostics, prognostics, and response to treatment. Clin Chem 2009, 55:1471-1483.
3. Wojdacz T.K., Dobrovic A. Methylation-sensitive high resolution melting (MS-HRM): a new approach for sensitive and high-throughput assessment of methylation. Nucleic Acids Res 2007, 35:e41.
4. Sriraksa R., Zeller C., El-Bahrawy M.A., Dai W., Daduang J., Jearanaikoon P., et al. CpG-island methylation study of liver fluke-related cholangiocarcinoma. Br J Cancer 2011, 104:1313-1318.
5. Sriraksa R., Chaopatchayakul P., Jearanaikoon P., Leelayuwat C., Limpaiboon T. Verification of complete bisulfite modification using Calponin-specific primer sets. Clin Biochem 2010, 43:528-530.
6. Tse M.Y., Ashbury J.E., Zwingerman N., King W.D., Taylor S.A., Pang S.C. A refined, rapid and reproducible high resolution melt (HRM)-based method suitable for quantification of global LINE-1 repetitive element methylation. BMC Res Notes 2011, 4:565.
7. Smith E., Jones M.E., Drew P.A. Quantitation of DNA methylation by melt curve analysis. BMC Cancer 2009, 9:123.
8. Candiloro I.L., Mikeska T., Dobrovic A. Assessing combined methylation-sensitive high resolution melting and pyrosequencing for the analysis of heterogeneous DNA methylation. Epigenetics 2011, 6:500-507.
9. Wojdacz T.K. Methylation-sensitive high-resolution melting in the context of legislative requirements for validation of analytical procedures for diagnostic applications. Expert Rev Mol Diagn 2012, 12:39-47.
10. Liu W., Guan M., Su B., Li J., Ma W., Liu C., et al. Rapid determination of AKAP12 promoter methylation levels in peripheral blood using methylation-sensitive high resolution melting (MS-HRM) analysis: application in colorectal cancer. Clin Chim Acta 2010, 411:940-946.