High-level expression in Corynebacterium glutamicum of nitrile hydratase from Rhodococcus rhodochrous for acrylamide production

Applied Microbiology and Biotechnology

Volumn 98, Issue 10, 2014, Pages 4379-4387

  Kang, Mi Suk ^a   Han, Sang Soo ^b   Kim, Mi Young ^c   Kim, Bu Youn ^c   Huh, Jong Pil ^c   Kim, Hak Sung ^b   Lee, Jin Ho ^a  

^a Kyungsung University   (South Korea)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^c Tong Suh Petrochemical Corp Ltd   (South Korea)

Author keywords

Acrylamide; Corynebacterium glutamicum; Nitrile hydratase; Rhodococcus rhodochrous

Indexed keywords

BATCH CELL CULTURE; CELLS; CLONING; CYANIDES; CYTOLOGY; ENZYME ACTIVITY;

ACRYLAMIDES; CORYNEBACTERIUM GLUTAMICUM; EXPRESSION LEVELS; FED-BATCH CULTURES; HIGH LEVEL EXPRESSION; NITRILE HYDRATASES; RHODOCOCCUS RHODOCHROUS; TRANSLATIONAL INITIATION;

AMIDES;

ACRYLAMIDE; ACRYLONITRILE; NITRILE HYDRATASE; HYDROLYASE; MUTANT PROTEIN; RECOMBINANT PROTEIN;

BACTERIUM; ENZYME ACTIVITY; GENE EXPRESSION; MUTATION; RECOMBINATION;

ARTICLE; BACTERIAL CELL; BACTERIAL GENE; BIOTRANSFORMATION; CELL DENSITY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CORYNEBACTERIUM GLUTAMICUM; DRY WEIGHT; ENZYME ACTIVITY; ENZYME SYNTHESIS; FED BATCH CULTURE; GENE MUTATION; ILVC GENE; MOLECULAR CLONING; NHHBAG GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; RHODOCOCCUS RHODOCHROUS; SEQUENCE ANALYSIS; TRANSLATION INITIATION; WILD TYPE; ENZYMOLOGY; GENE EXPRESSION; GENETICS; METABOLISM; PLASMID; RHODOCOCCUS;

CORYNEBACTERIUM; CORYNEBACTERIUM GLUTAMICUM; RHODOCOCCUS RHODOCHROUS;

ACRYLAMIDE; CLONING, MOLECULAR; CORYNEBACTERIUM GLUTAMICUM; GENE EXPRESSION; HYDRO-LYASES; MUTANT PROTEINS; PLASMIDS; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; RHODOCOCCUS;

EID: 84900789966     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-014-5544-7     Document Type: Article

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