Functional expression of nitrile hydratase in Escherichia coli: Requirement of a nitrile hydratase activator and post-translational modification of a ligand cysteine

Journal of Biochemistry

Volumn 125, Issue 4, 1999, Pages 696-704

  Nojiri, Masaki ^a,b   Yohda, Masafumi ^a,d   Odaka, Masafumi ^a   Matsushita, Yusuke ^a,c   Tsujimura, Masanari ^a,b,e   Yoshida, Takao ^a,b,f   Dohmae, Naoshi ^a   Takio, Koji ^a   Endo, Isao ^a  

^a INST OF PHYS AND CHEMICAL RESEARCH   (Japan)

^b SAITAMA UNIVERSITY   (Japan)

^c GAKUSHUIN UNIVERSITY   (Japan)

^d TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

^e UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^f MARINE BIOTECHNOLOGY INSTITUTE   (Japan)

Author keywords

Hydration; Metalloprotein; Nitrile; Overexpression; Post translational modification

Indexed keywords

CYSTEINE; ENZYME ACTIVATOR; IRON; NITRIC OXIDE; NITRILE HYDRATASE; RECOMBINANT ENZYME;

ARTICLE; ESCHERICHIA COLI; EXPRESSION VECTOR; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; PHOTOREACTIVITY; PROTEIN PROCESSING; RHODOCOCCUS;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; RHODOCOCCUS; RHODOCOCCUS SP.; RHODOCOCCUS SP. N-771; UNCULTURED ACTINOMYCETE;

EID: 0032904843     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a022339     Document Type: Article

References (40)

1. Kobayashi, M., Nagasawa, T., and Yamada, H. (1992) Enzymatic synthesis of acrylamide: a success story not yet over. Trends Biotechnol. 10, 402-408
2. Sugiura, Y., Kuwahara, T., Nagasawa, H., and Yamada, H. (1987) Nitrile hydratase: The first non-heme iron enzyme with a typical low-spin Fe(III)-active center. J. Am. Chem. Soc. 109, 5848-5850
3. Brennan, B.A., Alms, G., Nelson, M.J., Durney, L.T., and Scarrow, R.C. (1996) Nitrile hydratase from Rhodococcus rhodochrous J1 contains a non-corrinoid cobalt ion with two sulfur ligands. J. Am. Chem. Soc. 118, 9194-9195
4. Huang, W., Jia, J., Cummings, J., Nelson, M., Schneider, G., and Lindqvist, Y. (1997) Crystal structure of nitrile hydratase reveals. a novel iron centre in a novel fold. Structure 5, 691-699
5. Nagamune, T., Kurata, H., Hirata, M., Honda, J., Hirata, A., and Endo, I. (1990) Photosensitive phenomena of nitrile hydratase of Rhodococcus sp. N-771. Photochem. Photobiol. 51, 87-90
6. Noguchi, T., Honda, J., Nagamune, T., Sasabe, H., Inoue, Y., and Endo, I. (1995) Photosensitive nitrile hydratase intrinsically possesses nitric oxide bound to the non-heme iron center: evidence by fourier transform infrared spectroscopy. FEBS Lett. 358, 9-12
7. Odaka, M., Fujii, K., Hoshino, M., Noguchi, T., Tsujimura, M., Nagashima, S., Yohda, M., Nagamune, T., Inoue, Y., and Endo, I. (1997) Activity regulation of photoreactive nitrile hydratase by nitric oxide. J. Am. Chem. Soc. 119, 3785-3791
8. Noguchi, T., Hoshino, M., Tsujimura, M., Odaka, M., Inoue, Y., and Endo, I. (1996) Resonance Raman evidence that photodissociation of nitric oxide from the non-heme iron center activates nitrile hydratase from Rhodococcus sp. N-771. Biochemistry 35, 16777-16781
9. Bonnet, D., Artaud, I., Moali, C., Petre, D., and Mansuy, D. (1997) Highly efficient control of iron-containing nitrile hydratases by stoichiometric amounts of nitric oxide and light. FEBS Lett. 409, 216-220
10. Nagashima, S., Nakasako, M., Dohmae, N., Tsujimura, M., Takio, K., Odaka, M., Yohda, M., Kamiya, N., and Endo, I. (1998) Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms. Nat. Struct. Biol. 5, 347-351
11. Tsujimura, M., Dohmae, N., Odaka, M., Chijimatsu, M., Takio, K., Yohda, M., Hoshino, M., Nagashima, S., and Endo, I. (1997) Structure of the photoreactive iron center of the nitrile hydratase from Rhodococcus sp. N-771. J. Biol. Chem. 272, 29454-29459
12. Ikehata, O., Nishiyama, M., Horinouchi, S., and Beppu, T. (1989) Primary structure of nitrile hydratase deduced from the nucleotide sequence of a Rhodococcus species and its expression in Escherichia coli. Eur. J. Biochem. 181, 563-570
13. Hashimoto, Y., Nishiyama, M., Horinouchi, S., and Beppu, T. (1994) Nitrile hydratase gene from Rhodococcus sp. N-774 requirement for its downstream region for efficient expression. Biosci. Biotechnol. Biochem. 58, 1859-1865
14. Nishiyama, M., Horinouchi, S., Kobayashi, M., Nagasawa, T., Yamada, H., and Beppu, T. (1991) Cloning and characterization of genes responsible for metabolism of nitrile compounds from Pseudomonas chlororaphis B23. J. Bacteriol. 173, 2465-2472
15. Komeda, H., Kobayashi, M., and Shimizu, S. (1996) Characterization of the gene cluster of high-molecular-mass nitrile hydratase (H-NHase) induced by its reaction product in Rhodococcus rhodochrous J1. Proc. Natl. Acad. Sci. USA 93, 4267-4272
16. Komeda, H., Kobayashi, M., and Shimizu, S. (1997) A novel transporter involved in cobalt uptake. Proc. Natl. Acad. Sci. USA 94, 36-41
17. Wu, S., Fallon, R.D., and Payne, M.S. (1997) Over-production of stereoselective nitrile hydratase from Pseudomonas putida 5B in Escherichia coli: activity requires a novel downstream protein. Appl. Microbiol. Biotechnol. 48, 704-708
18. Yohda, M., Honda, J., Nagamune, T., Endo, I., Yoshida, T., and Miura, K. (1994) Molecular cloning and nucleotide sequence of the gene coding photosensitive nitrile hydratase. Ann. N.Y. Acad. Sci. 721, 158-159
19. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
20. Brennan, B.A., Cummings, J.G., Chase, D.B., Turner, I.M. Jr., and Nelson, M.J. (1996) Resonance raman spectroscopy of nitrile hydratase, a novel iron-sulfur enzyme. Biochemistry 35, 10068-10077
21. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254
22. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685
23. Grzeszik, C., Lubbers, M., Reh, M., and Schlegel, H.G. (1997) Genes encoding the NAD-reducing hydrogenase of Rhodococcus opacus MR11. Microbiology 143, 1271-1286
24. Komeda, H., Kobayashi, M., and Shimizu, S. (1996) A novel gene cluster including the Rhodococcus rhodochrous J1 nhlBA genes encoding a low molecular mass nitrile hydratase (L-NHase) induced by its reaction product. J. Biol. Chem. 271, 15796-15802
25. Wilson, S. and Drew, R. (1991) Cloning and DNA sequence of amiC, a new gene regulating expression of the Pseudomonas aeruginosa aliphatic amidase, and purification of the amiC product. J. Bacteriol. 173, 4914-4921
26. Nagasawa, T., Ryuno, K., and Yamada, H. (1986) Nitrile hydratase of Brevibacterium R312. Biochem. Biophys. Res. Commun. 139, 1305-1312
27. Tsujimura, M., Odaka, M., Nagashima, S., Yohda, M., and Endo, I. (1996) Photoreactive nitrile hydratase: the photoreaction site is located on the a subunit. J. Biochem. 119, 407-413
28. Tourneix, D., Thiery, A., Maestracci, M., Arnaud, A., and Galzy, P. (1986) Regulation of nitrile-hydratase synthesis in a Brevibacterium species. Antonie Van Leeuwenhoek. 52, 173-182
29. Nakamura, C., Burgess, J.G., Sode, K., and Matsunaga, T. (1995) An iron-regulated gene, magA, encoding an iron transport protein of Magnetospirillum sp. strain AMB-1. J. Biol. Chem. 270, 28392-28396
30. Asahi, M., Fujii, J., Suzuki, K., Seo, H.G., Kuzuya, T., Hori, M., Tada, M., Fujii, S., and Taniguchi, N. (1995) Inactivation of glutathione peroxidase by nitric oxide. J. Biol. Chem. 270, 21035-21039
31. Asahi, M., Fujii, J., Takao, T., Kuzuya, T., Hori, M., Shimonishi, Y., and Taniguchi, N. (1997) The oxidation of selenocysteine is involved in the inactivation of glutathione peroxidase by nitric oxide donor. J. Biol. Chem. 272, 19152-19157
32. Radi, R., Bechman, J.S., Bush, K.M., and Freeman, B.A. (1991) Peroxynitrite oxidation of sulfhydryls. J. Biol. Chem. 266, 4244-4250
33. Ischiropoulos, H. and Al-Mehdi, A.B. (1995) Peroxynitrite-mediated oxidative protein modifications. FEBS Lett. 364, 279-282
34. DeMaster, E.G., Quast, B.J., Redfern, B., and Nagasawa, H.T. (1995) Reaction of nitric oxide with the free sulfhydryl group of human serum albumin yields a sulfenic acid and nitrous oxide. Biochemistry 34, 11494-11499
35. Hosokawa, Y., Matsumoto, A., Oka, J., Itakura, H., and Yamaguchi, K. (1990) Isolation and characterization of a cDNA for rat liver cysteine dioxygenase. Biochem. Biophys. Res. Commun. 168, 473-478
36. McCann, K.P., Akbari, M.T., Williams, A.C., and Ramsden, D.B. (1994) Human cysteine dioxygenase type I: primary structure derived from base sequencing of cDNA. Biochim. Biophys. Acta 1209, 107-110
37. Matsuzaki, R., Fukui, T., Sato, H., Ozaki, Y., and Tanizawa, K. (1994) Generation of the topa quinone cofactor in bacterial monoamine oxidase by cupric ion-dependent autooxidation of a specific tyrosyl residue. FEBS Lett. 351, 360-364
38. Matsuzaki, R., Suzuki, S., Yamaguchi, K., Fukui, T., and Tanizawa, K. (1995) Spectroscopic studies on the mechanism of the topa quinone generation in bacterial monoamine oxidase. Biochemistry 34, 4524-4530
39. Nakamura, N., Matsuzaki, R., Choi, Y.H., Tanizawa, K., and Sanders-Loehr, J. (1996) Biosynthesis of topa quinone cofactor in bacterial amine oxidase. J. Biol. Chem. 271, 4718-4724
40. Cai, D. and Klinman, J.P. (1994) Copper amine oxidase: heterologous expression, purification, and characterization of an active enzyme in Saccharomyces cerevisiae. Biochemistry 33, 7647-7653