Nitrile hydratase from Rhodococcus rhodochrous J1 contains a non-corrin cobalt ion with two sulfur ligands

Journal of the American Chemical Society

Volumn 118, Issue 38, 1996, Pages 9194-9195

  Brennan, Bridget A ^a   Alms, Geoffrey ^a   Nelson, Mark J ^a   Durney, Lewellyn T ^b   Scarrow, Robert C ^b  

^a DUPONT COMPANY   (United States)

^b HAVERFORD COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME; HYDROLYASE;

ARTICLE; ELECTRON SPIN RESONANCE; ENZYME ACTIVITY; ENZYME ANALYSIS; INFRARED SPECTROPHOTOMETRY; PHOTOTROPHIC BACTERIUM;

EID: 0029805756     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja961920d     Document Type: Article

References (36)

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12. 10 Samples recovered after irradiation retained 90% of their activity.
13. Samples were added to 1 M nitric acid (Ultrapure nitric acid. J. T. Baker) and heated in closed containers at 90 °C for 24 h. The liquid was allowed to evaporate, and the samples were taken up in 0.5 mL of 1 M nitric acid. The concentration of cobalt in each sample was quantified in triplicate using a Perkin-Elmer 3300 atomic absorption spectrophotometer.
14. Purified nitrile hydratase was hydrolyzed by treatment with 6 N HCl for 24 h at 100 °C, and the total amino acid composition was determined using a Beckman System 6300 amino acid analyzer.
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