Continuous processing of recombinant proteins: Integration of refolding and purification using simulated moving bed size-exclusion chromatography with buffer recycling

Journal of Chromatography A

Volumn 1337, Issue , 2014, Pages 48-56

  Wellhoefer, Martin ^a   Sprinzl, Wolfgang ^a   Hahn, Rainer ^a,b   Jungbauer, Alois ^a,b  

^a AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

^b UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

Autoprotease fusion protein; Buffer recycling; Continuous purification; Process intensification; Simulated moving bed

Indexed keywords

CONTINUOUS PROCESS; CONTINUOUS PROCESSING; FUSION PROTEINS; PROCESS INTENSIFICATION; RESIDUAL PROTEINS; SIMULATED MOVING BED; SIMULATED MOVING BEDS; TANGENTIAL FLOW FILTRATIONS;

BATCH DATA PROCESSING; COLUMN CHROMATOGRAPHY; PEPTIDES; PRODUCTIVITY; RECOMBINANT PROTEINS; RECYCLING; SIZE EXCLUSION CHROMATOGRAPHY;

PURIFICATION;

AUTOPROTEASE; BUFFER; HISTIDINE; HYBRID PROTEIN; MONOCYTE CHEMOTACTIC PROTEIN 1; PEPTIDE; PROTEINASE; RECOMBINANT PROTEIN; SODIUM HYDROXIDE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; BATCH PROCESS; BUFFER RECYCLING; CELL INCLUSION; ESCHERICHIA COLI; FLOW RATE; GEL PERMEATION CHROMATOGRAPHY; NONHUMAN; PHASE SEPARATION; PRIORITY JOURNAL; PRODUCTIVITY; PROTEIN CLEAVAGE; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEIN REFOLDING; SIMULATED MOVING BED SIZE EXCLUSION CHROMATOGRAPHY; TANGENTIAL FLOW FILTRATION;

AUTOPROTEASE FUSION PROTEIN; BUFFER RECYCLING; CONTINUOUS PURIFICATION; PROCESS INTENSIFICATION; SIMULATED MOVING BED;

BUFFERS; CHROMATOGRAPHY, GEL; FEASIBILITY STUDIES; INCLUSION BODIES; PEPTIDES; PROTEIN REFOLDING; RECOMBINANT FUSION PROTEINS;

EID: 84900589272     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2014.02.016     Document Type: Article

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