Improving kinetic or thermodynamic stability of an azoreductase by directed evolution

PLoS ONE

Volumn 9, Issue 1, 2014, Pages

  Brissos, Vânia ^a   Gonçalves, Nádia ^a   Melo, Eduardo P ^b   Martins, Lígia O ^a  

^a INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^b UNIVERSITY OF ALGARVE   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

AZO REDUCTASE;

AMINO ACID SUBSTITUTION; ARTICLE; BIOREMEDIATION; CALORIMETRY; CONTROLLED STUDY; CRYSTAL STRUCTURE; DIFFERENTIAL SCANNING CALORIMETRY; DNA MODIFICATION; ENZYME KINETICS; ENZYME STABILITY; HIGH THROUGHPUT SCREENING; HYDROPHOBICITY; LIGHT SCATTERING; MOLECULAR EVOLUTION; NONHUMAN; PROTEIN AGGREGATION; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN STABILITY; PSEUDOMONAS PUTIDA; SITE DIRECTED MUTAGENESIS; THERMODYNAMICS; THERMOSTABILITY;

AMINO ACIDS; DIRECTED MOLECULAR EVOLUTION; DNA PRIMERS; ENZYME STABILITY; ESCHERICHIA COLI; HALF-LIFE; HIGH-THROUGHPUT SCREENING ASSAYS; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; NADH, NADPH OXIDOREDUCTASES; PROTEIN CONFORMATION; TEMPERATURE; THERMODYNAMICS;

EID: 84900338484     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0087209     Document Type: Article

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