Recombinant production of the amino terminal cytoplasmic region of dengue virus non-structural protein 4A for structural studies

PLoS ONE

Volumn 9, Issue 1, 2014, Pages

  Hung, Yu Fu ^b   Valdau, Olga ^a   Schünke, Sven ^a   Stern, Omer ^c   Koenig, Bernd W ^a,b   Willbold, Dieter ^a,b   Hoffmann, Silke ^a  

^a FORSCHUNGSZENTRUM JÜLICH   (Germany)

^b HEINRICH HEINE UNIVERSITY   (Germany)

^c TEL AVIV UNIVERSITY   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; ESCHERICHIA COLI PROTEIN; GLUTAMIC ACID; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; LEUCINE; METHIONINE; MUTANT PROTEIN; NONSTRUCTURAL PROTEIN 4A; PEPTIDE DERIVATIVE; PROTEIN GB1; PROTEINASE; SYNTHETIC DNA; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CODON; CONTROLLED STUDY; CYTOPLASM; DENGUE VIRUS; ESCHERICHIA COLI; NONHUMAN; PROTEIN CLEAVAGE; PROTEIN DEFECT; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; TOBACCO VIRUS; VIRUS STRAIN;

AMINO ACID SEQUENCE; ANTIVIRAL AGENTS; BASE SEQUENCE; BLOTTING, WESTERN; DENGUE; DENGUE VIRUS; ENDOPEPTIDASES; ESCHERICHIA COLI; GLUTATHIONE TRANSFERASE; HUMANS; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR SEQUENCE DATA; MUTANT PROTEINS; MUTATION; PEPTIDE FRAGMENTS; PLASMIDS; RECOMBINANT FUSION PROTEINS; SEQUENCE HOMOLOGY, NUCLEIC ACID; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; UBIQUITIN; VIRAL NONSTRUCTURAL PROTEINS; VIRUS REPLICATION;

EID: 84899813573     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0086482     Document Type: Article

References (40)

1. Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, et al. (2013) The global distribution and burden of dengue. Nature.
2. Clyde K, Kyle JL, Harris E (2006) Recent advances in deciphering viral and host determinants of dengue virus replication and pathogenesis. J Virol 80: 11418-11431. (Pubitemid 44788858)
3. Miller S, Kastner S, Krijnse-Locker J, Buhler S, Bartenschlager R (2007) The non-structural protein 4A of dengue virus is an integral membrane protein inducing membrane alterations in a 2K-regulated manner. J Biol Chem 282: 8873-8882. (Pubitemid 47093477)
4. Mackenzie JM, Khromykh AA, Jones MK, Westaway EG (1998) Subcellular localization and some biochemical properties of the flavivirus Kunjin nonstructural proteins NS2A and NS4A. Virology 245: 203-215. (Pubitemid 28394183)
5. Roosendaal J, Westaway EG, Khromykh A, Mackenzie JM (2006) Regulated cleavages at the West Nile virus NS4A-2K-NS4B junctions play a major role in rearranging cytoplasmic membranes and Golgi trafficking of the NS4A protein. J Virol 80: 4623-4632.
6. Stern O, Hung YF, Valdau O, Yaffe Y, Harris E, et al. (2013) An N-terminal amphipathic helix in the Dengue virus nonstructural protein 4A mediates oligomerization and is essential for replication. J Virol.
7. Sapay N, Guermeur Y, Deleage G (2006) Prediction of amphipathic in-plane membrane anchors in monotopic proteins using a SVM classifier. BMC Bioinformatics 7: 255.
8. Jao CC, Hegde BG, Gallop JL, Hegde PB, McMahon HT, et al. (2010) Roles of amphipathic helices and the bin/amphiphysin/rvs (BAR) domain of endophilin in membrane curvature generation. J Biol Chem 285: 20164-20170.
9. Lee MC, Orci L, Hamamoto S, Futai E, Ravazzola M, et al. (2005) Sar1p N-terminal helix initiates membrane curvature and completes the fission of a COPII vesicle. Cell 122: 605-617. (Pubitemid 41191158)
10. McMahon HT, Gallop JL (2005) Membrane curvature and mechanisms of dynamic cell membrane remodelling. Nature 438: 590-596. (Pubitemid 41740562)
11. Elazar M, Cheong KH, Liu P, Greenberg HB, Rice CM, et al. (2003) Amphipathic helix-dependent localization of NS5A mediates hepatitis C virus RNA replication. J Virol 77: 6055-6061. (Pubitemid 36546191)
12. Elazar M, Liu P, Rice CM, Glenn JS (2004) An N-terminal amphipathic helix in hepatitis C virus (HCV) NS4B mediates membrane association, correct localization of replication complex proteins, and HCV RNA replication. J Virol 78: 11393-11400. (Pubitemid 39299681)
13. Gouttenoire J, Castet V, Montserret R, Arora N, Raussens V, et al. (2009) Identification of a novel determinant for membrane association in hepatitis C virus nonstructural protein 4B. J Virol 83: 6257-6268.
14. Penin F, Brass V, Appel N, Ramboarina S, Montserret R, et al. (2004) Structure and function of the membrane anchor domain of hepatitis C virus nonstructural protein 5A. J Biol Chem 279: 40835-40843. (Pubitemid 39287681)
15. Spuul P, Salonen A, Merits A, Jokitalo E, Kaariainen L, et al. (2007) Role of the amphipathic peptide of Semliki forest virus replicase protein nsP1 in membrane association and virus replication. J Virol 81: 872-883. (Pubitemid 46067912)
16. Teterina NL, Gorbalenya AE, Egger D, Bienz K, Rinaudo MS, et al. (2006) Testing the modularity of the N-terminal amphipathic helix conserved in picornavirus 2C proteins and hepatitis C NS5A protein. Virology 344: 453-467. (Pubitemid 43049632)
17. Cho NJ, Dvory-Sobol H, Lee C, Cho SJ, Bryson P, et al. (2010) Identification of a class of HCV inhibitors directed against the nonstructural protein NS4B. Sci Transl Med 2: 15ra16.
18. Koenig BW, Rogowski M, Louis JM (2003) A rapid method to attain isotope labeled small soluble peptides for NMR studies. J Biomol NMR 26: 193-202. (Pubitemid 36758440)
19. Kohno T, Xiang L, Inaoka Y, Hayashi K, Suzuki C, et al. (2008) High-Efficiency and Robust Expression System for Stable Isotope-Labeled Peptides. Int J Pept Res Ther 14: 157-165.
20. Kohno T, Kusunoki H, Sato K, Wakamatsu K (1998) A new general method for the biosynthesis of stable isotope-enriched peptides using a decahistidine-tagged ubiquitin fusion system: an application to the production of mastoparan-X uniformly enriched with 15N and 15N/13C. J Biomol NMR 12: 109-121. (Pubitemid 128512837)
21. Fath S, Bauer AP, Liss M, Spriestersbach A, Maertens B, et al. (2011) Multiparameter RNA and codon optimization: a standardized tool to assess and enhance autologous mammalian gene expression. PLoS One 6: e17596.
22. Huth JR, Bewley CA, Jackson BM, Hinnebusch AG, Clore GM, et al. (1997) Design of an expression system for detecting folded protein domains and mapping macromolecular interactions by NMR. Protein Sci 6: 2359-2364. (Pubitemid 27490746)
23. Hemsley A, Arnheim N, Toney MD, Cortopassi G, Galas DJ (1989) A simple method for site-directed mutagenesis using the polymerase chain reaction. Nucleic Acids Res 17: 6545-6551. (Pubitemid 19215577)
24. Kapust RB, Tozser J, Fox JD, Anderson DE, Cherry S, et al. (2001) Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Protein Eng 14: 993-1000. (Pubitemid 34137240)
25. Favier A, Brutscher B (2011) Recovering lost magnetization: polarization enhancement in biomolecular NMR. J Biomol NMR 49: 9-15.
26. Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, et al. (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6: 277-293.
27. Vranken WF, Boucher W, Stevens TJ, Fogh RH, Pajon A, et al. (2005) The CCPN data model for NMR spectroscopy: development of a software pipeline. Proteins 59: 687-696. (Pubitemid 40695845)
28. Kane JF (1995) Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli. Curr Opin Biotechnol 6: 494-500.
29. Bao WJ, Gao YG, Chang YG, Zhang TY, Lin XJ, et al. (2006) Highly efficient expression and purification system of small-size protein domains in Escherichia coli for biochemical characterization. Protein Expr Purif 47: 599-606.
30. Kapust RB, Tozser J, Copeland TD, Waugh DS (2002) The P19 specificity of tobacco etch virus protease. Biochem Biophys Res Commun 294: 949-955. (Pubitemid 34687141)
31. Kaplan W, Husler P, Klump H, Erhardt J, Sluis-Cremer N, et al. (1997) Conformational stability of pGEX-expressed Schistosoma japonicum glutathione S-transferase: a detoxification enzyme and fusion-protein affinity tag. Protein Sci 6: 399-406. (Pubitemid 27079932)
32. Lim K, Ho JX, Keeling K, Gilliland GL, Ji X, et al. (1994) Three-dimensional structure of Schistosoma japonicum glutathione S-transferase fused with a sixamino acid conserved neutralizing epitope of gp41 from HIV. Protein Sci 3: 2233-2244.
33. McTigue MA, Williams DR, Tainer JA (1995) Crystal structures of a schistosomal drug and vaccine target: glutathione S-transferase from Schistosoma japonica and its complex with the leading antischistosomal drug praziquantel. J Mol Biol 246: 21-27.
34. Niedziela-Majka A, Rymarczyk G, Kochman M, Ozyhar A (1998) GST-Induced dimerization of DNA-binding domains alters characteristics of their interaction with DNA. Protein Expr Purif 14: 208-220.
35. Qiu W, Derr ND, Goodman BS, Villa E, Wu D, et al. (2012) Dynein achieves processive motion using both stochastic and coordinated stepping. Nat Struct Mol Biol 19: 193-200.
36. Tudyka T, Skerra A (1997) Glutathione S-transferase can be used as a C-terminal, enzymatically active dimerization module for a recombinant protease inhibitor, and functionally secreted into the periplasm of Escherichia coli. Protein Sci 6: 2180-2187. (Pubitemid 27449297)
37. Sun C, Liang J, Shi R, Gao X, Zhang R, et al. (2012) Tobacco etch virus protease retains its activity in various buffers and in the presence of diverse additives. Protein Expr Purif 82: 226-231.
38. Waugh DS (2011) An overview of enzymatic reagents for the removal of affinity tags. Protein Expr Purif 80: 283-293.
39. Smith DB, Corcoran LM (2001) Expression and purification of glutathione-Stransferase fusion proteins. Curr Protoc Mol Biol Chapter 16: Unit16 17.
40. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, et al. (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947-2948. (Pubitemid 350162903)