A new general method for the biosynthesis of stable isotope-enriched peptides using a decahistidine-tagged ubiquitin fusion system: An application to the production of mastoparan-X uniformly enriched with 15N and 15N/13C

Journal of Biomolecular NMR

Volumn 12, Issue 1, 1998, Pages 109-121

  Kohno, Toshiyuki ^a   Kusunoki, Hideki ^a,b   Sato, Kazuki ^a   Wakamatsu, Kaori ^b,c  

^a MITSUBISHI KASEI INST OF LIFE SCI   (Japan)

^b GUNMA UNIVERSITY   (Japan)

^c INST OF PHYS AND CHEMICAL RESEARCH   (Japan)

Author keywords

Biosynthetic peptide; Mastoparan X; Stable isotope enrichment; Triple resonance NMR; Ubiquitin fusion system; Yeast ubiquitin hydrolase

Indexed keywords

ESCHERICHIA COLI; SACCHAROMYCES CEREVISIAE;

CARBON; HISTIDINE; HYBRID PROTEIN; MASTOPARAN X; NITROGEN; PEPTIDE; PRIMER DNA; UBIQUITIN; WASP VENOM;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CHEMISTRY; CIRCULAR DICHROISM; GENETICS; ISOTOPE LABELING; METHODOLOGY; MOLECULAR CLONING; NUCLEAR MAGNETIC RESONANCE; PEPTIDE SYNTHESIS; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION; SACCHAROMYCES CEREVISIAE;

AMINO ACID SEQUENCE; CARBON ISOTOPES; CIRCULAR DICHROISM; CLONING, MOLECULAR; DNA PRIMERS; HISTIDINE; ISOTOPE LABELING; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PEPTIDE BIOSYNTHESIS; PEPTIDES; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; UBIQUITINS; WASP VENOMS;

EID: 0032111031     PISSN: 09252738     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008254603368     Document Type: Article

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