Culturing of mouse and human cells on soft substrates promote the expression of stem cell markers

Journal of Bioscience and Bioengineering

Volumn 117, Issue 6, 2014, Pages 749-755

  Higuchi, Sayaka ^a   Watanabe, Tomonobu M ^a,b   Kawauchi, Keiko ^c   Ichimura, Taro ^a   Fujita, Hideaki ^a,b  

^a RIKEN Center for Biosystems Dynamics Research   (Japan)

^b OSAKA UNIVERSITY   (Japan)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Actin filament; Elasticity; Fibroblasts; Induced pluripotent stem cells; Reprogramming

Indexed keywords

CELL CULTURE; ELASTICITY; FIBROBLASTS; GENES; PROTEINS; STEM CELLS;

ACTIN FILAMENT; ARTIFICIAL SUBSTRATES; HUMAN DERMAL FIBROBLASTS; INDUCED PLURIPOTENT STEM CELLS; MOUSE EMBRYONIC FIBROBLASTS; MOUSE EMBRYONIC STEM CELLS; REPROGRAMMING; RETROVIRAL INFECTIONS;

SUBSTRATES;

CELL MARKER; GELATIN; KRUPPEL LIKE FACTOR 4; OCTAMER TRANSCRIPTION FACTOR 4; ORGANIC CATION TRANSPORTER 3; PLASTIC; POLYACRYLAMIDE GEL; RETROVIRUS VECTOR; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR LIN28; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR ROCK2; TRANSCRIPTION FACTOR SOX2; UNCLASSIFIED DRUG; ACRYLAMIDE DERIVATIVE; BIOLOGICAL MARKER; CULTURE MEDIUM; HOMEODOMAIN PROTEIN; NANOG PROTEIN, MOUSE; POU5F1 PROTEIN, MOUSE; REX-1 PROTEIN, MOUSE; RHO KINASE; ROCK2 PROTEIN, MOUSE; SOX2 PROTEIN, MOUSE; TRANSCRIPTION FACTOR SOX;

ADULT; ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; CONTROLLED STUDY; DOWN REGULATION; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IMMUNOFLUORESCENCE MICROSCOPY; MOUSE; NONHUMAN; NUCLEAR REPROGRAMMING; PLURIPOTENT STEM CELL; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SKIN FIBROBLAST; SOMATIC CELL; STRESS FIBER; UPREGULATION; ADULT STEM CELL; ANIMAL; CELL CULTURE; CELL DIFFERENTIATION; CHEMISTRY; CULTURE MEDIUM; CULTURE TECHNIQUE; FIBROBLAST; GENETICS; HARDNESS; METABOLISM; PHYSIOLOGY;

IPS;

ACRYLAMIDES; ADULT STEM CELLS; ANIMALS; BIOLOGICAL MARKERS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELLS, CULTURED; CULTURE MEDIA; FIBROBLASTS; GENE EXPRESSION; HARDNESS; HOMEODOMAIN PROTEINS; HUMANS; INDUCED PLURIPOTENT STEM CELLS; MICE; OCTAMER TRANSCRIPTION FACTOR-3; RHO-ASSOCIATED KINASES; SOXB1 TRANSCRIPTION FACTORS; TRANSCRIPTION FACTORS;

EID: 84899639533     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2013.11.011     Document Type: Article

References (35)

1. Takahashi K., Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006, 126:663-676.
2. Hong H., Takahashi K., Ichisaka T., Aoi T., Kanagawa O., Nakagawa M., Okita K., Yamanaka S. Suppression of induced pluripotent stem cell generation by the p53-p21 pathway. Nature 2009, 460:1132-1135.
3. Yoshida Y., Takahashi K., Okita K., Ichisaka T., Yamanaka S. Hypoxia enhances the generation of induced pluripotent stem cells. Cell Stem Cell 2009, 5:237-241.
4. Tchieu J., Kuoy E., Chin M.H., Trinh H., Patterson M., Sherman S.P., Aimiuwu O., Lindgren A., Hakimian S., Zack J.A., other 4 authors Female human iPSCs retain an inactive X chromosome. Cell Stem Cell 2010, 7:329-342.
5. Marchetto M.C., Carromeu C., Acab A., Yu D., Yeo G.W., Mu Y., Chen G., Gage F.H., Muotri A.R. Amodel for neural development and treatment of Rett syndrome using human induced pluripotent stem cells. Cell 2010, 143:527-539.
6. Okita K., Matsumura Y., Sato Y., Okada A., Morizane A., Okamoto S., Hong H., Nakagawa M., Tanabe K., Tezuka K., other 6 authors Amore efficient method to generate integration-free human iPS cells. Nat. Methods 2011, 8:409-412.
7. Zhou H., Wu S., Joo J.Y., Zhu S., Han D.W., Lin T., Trauger S., Bien G., Yao S., Zhu Y., other 4 authors Generation of induced pluripotent stem cells using recombinant proteins. Cell Stem Cell 2009, 4:381-384.
8. Hayashi Y., Furue M.K., Okamoto T., Ohnuma K., Myoishi Y., Fukuhara Y., Abe T., Sato J.D., Hata R., Asashima M. Integrins regulate mouse embryonic stem cell self-renewal. Stem Cells 2007, 25:3005-3015.
9. Guilak F., Cohen D.M., Estes B.T., Gimble J.M., Liedtke W., Chen C.S. Control of stem cell fate by physical interactions with the extracellular matrix. Cell Stem Cell 2009, 5:17-26.
10. Higuchi S., Lin Q., Wang J., Lim T.K., Joshi S.B., Anand G.S., Chung M.C., Sheetz M.P., Fujita H. Heart extracellular matrix supports cardiomyocyte differentiation of mouse embryonic stem cells. J.Biosci. Bioeng. 2013, 115:320-325.
11. Engler A.J., Sen S., Sweeney H.L., Discher D.E. Matrix elasticity directs stem cell lineage specification. Cell 2006, 126:677-689.
12. Chowdhury F., Li Y., Poh Y.C., Yokohama-Tamaki T., Wang N., Tanaka T.S. Soft substrates promote homogeneous self-renewal of embryonic stem cells via downregulating cell-matrix tractions. PLoS One 2010, 5:e15655.
13. Liao J., Wu Z., Wang Y., Cheng L., Cui C., Gao Y., Chen T., Rao L., Chen S., Jia N., other 5 authors Enhanced efficiency of generating induced pluripotent stem (iPS) cells from human somatic cells by a combination of six transcription factors. Cell Res. 2008, 18:600-603.
14. Takahashi K., Tanabe K., Ohnuki M., Narita M., Ichisaka T., Tomoda K., Yamanaka S. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 2007, 131:861-872.
15. Yu J., Vodyanik M.A., Smuga-Otto K., Antosiewicz-Bourget J., Frane J.L., Tian S., Nie J., Jonsdottir G.A., Ruotti V., Stewart R., Slukvin I.I., Thomson J.A. Induced pluripotent stem cell lines derived from human somatic cells. Science 2007, 318:1917-1920.
16. Gauthaman K., Fong C.Y., Bongso A. Effect of ROCK inhibitor Y-27632 on normal and variant human embryonic stem cells (hESCs) in vitro: its benefits in hESC expansion. Stem Cell Rev. 2010, 6:86-95.
17. Zhang L., Valdez J.M., Zhang B., Wei L., Chang J., Xin L. ROCK inhibitor Y-27632 suppresses dissociation-induced apoptosis of murine prostate stem/progenitor cells and increases their cloning efficiency. PLoS One 2011, 6:e18271.
18. Zhu S., Li W., Zhou H., Wei W., Ambasudhan R., Lin T., Kim J., Zhang K., Ding S. Reprogramming of human primary somatic cells by OCT4 and chemical compounds. Cell Stem Cell 2010, 7:651-655.
19. Tse J.R., Engler A.J. Preparation of hydrogel substrates with tunable mechanical properties. Curr. Protoc. Cell Biol. 2010, 16. Chapter 10, Unit 10.
20. Sawada K., Krantz S.B., Kans J.S., Dessypris E.N., Sawyer S., Glick A.D., Civin C.I. Purification of human erythroid colony-forming units and demonstration of specific binding of erythropoietin. J.Clin. Invest. 1987, 80:357-366.
21. Zhao W., Ji X., Zhang F., Li L., Ma L. Embryonic stem cell markers. Molecules 2012, 17:6196-6236.
22. Shemesh T., Geiger B., Bershadsky A.D., Kozlov M.M. Focal adhesions as mechanosensors: a physical mechanism. Proc. Natl. Acad. Sci. USA 2005, 102:12383-12388.
23. Pelham R.J., Wang Y. Cell locomotion and focal adhesions are regulated by substrate flexibility. Proc. Natl. Acad. Sci. USA 1997, 94:13661-13665.
24. Machesky L.M., Hall A. Role of actin polymerization and adhesion to extracellular matrix in Rac- and Rho-induced cytoskeletal reorganization. J.Cell Biol. 1997, 138:913-926.
25. Etienne-Manneville S., Hall A. Rho GTPases in cell biology. Nature 2002, 420:629-635.
26. Maekawa M., Ishizaki T., Boku S., Watanabe N., Fujita A., Iwamatsu A., Obinata T., Ohashi K., Mizuno K., Narumiya S. Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase. Science 1999, 285:895-898.
27. Fukata Y., Amano M., Kaibuchi K. Rho-Rho-kinase pathway in smooth muscle contraction and cytoskeletal reorganization of non-muscle cells. Trends Pharmacol. Sci. 2001, 22:32-39.
28. Nakagawa O., Fujisawa K., Ishizaki T., Saito Y., Nakao K., Narumiya S. ROCK-I and ROCK-II, two isoforms of Rho-associated coiled-coil forming protein serine/threonine kinase in mice. FEBS Lett. 1996, 392:189-193.
29. Watanabe K., Ueno M., Kamiya D., Nishiyama A., Matsumura M., Wataya T., Takahashi J.B., Nishikawa S., Muguruma K., Sasai Y. AROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat. Biotechnol. 2007, 25:681-686.
30. Li X., Krawetz R., Liu S., Meng G., Rancourt D.E. ROCK inhibitor improves survival of cryopreserved serum/feeder-free single human embryonic stem cells. Hum. Reprod. 2009, 24:580-589.
31. Bhadriraju K., Yang M., Alom Ruiz S., Pirone D., Tan J., Chen C.S. Activation of ROCK by RhoA is regulated by cell adhesion, shape, and cytoskeletal tension. Exp. Cell Res. 2007, 313:3616-3623.
32. Katoh K., Kano Y., Amano M., Onishi H., Kaibuchi K., Fujiwara K. Rho-kinase-mediated contraction of isolated stress fibers. J.Cell Biol. 2001, 153:569-584.
33. McBeath R., Pirone D.M., Nelson C.M., Bhadriraju K., Chen C.S. Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Dev. Cell 2004, 6:483-495.
34. Rodriguez J.P., Gonzalez M., Rios S., Cambiazo V. Cytoskeletal organization of human mesenchymal stem cells (MSC) changes during their osteogenic differentiation. J.Cell. Biochem. 2004, 93:721-731.
35. Tanabe K., Nakamura M., Narita M., Takahashi K., Yamanaka S. Maturation, not initiation, is the major roadblock during reprogramming toward pluripotency from human fibroblasts. Proc. Natl. Acad. Sci. USA 2013, 110:12172-12179.