Development, validation and application of specific primers for analyzing the clostridial diversity in dark fermentation pit mud by PCR-DGGE

Bioresource Technology

Volumn 163, Issue , 2014, Pages 40-47

  Hu, Xiao Long ^a   Wang, Hai Yan ^a   Wu, Qun ^a   Xu, Yan ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Biodiversity; Clostridia specific primers; Complex microbial ecosystem; Dark fermentation pit mud; PCR DGGE

Indexed keywords

BIODIVERSITY; POLYMERASE CHAIN REACTION; RNA;

ANAEROBIC ENVIRONMENT; CLOSTRIDIA-SPECIFIC PRIMERS; COVERAGE RATE; DARK FERMENTATION; EXPERIMENTAL EVALUATION; MICROBIAL ECO SYSTEM; PCR-DGGE; SPECIFIC PRIMERS;

FERMENTATION;

BACTERIAL DNA; GENOMIC DNA; RNA 16S; PRIMER DNA;

BACTERIOLOGY; BACTERIUM; BIODIVERSITY; BIOTECHNOLOGY; DATABASE; FERMENTATION; MICROBIAL ECOLOGY; POLYMERASE CHAIN REACTION; POPULATION DYNAMICS; SPECIES DIVERSITY;

ALKALIBACTER; ARTICLE; BACILLUS LICHENIFORMIS; BACILLUS THURINGIENSIS; BACTERIUM CULTURE; BIOMASS; CLOSTRIDIA; CLOSTRIDIUM; CLOSTRIDIUM BUTYRICUM; CLOSTRIDIUM KLUYVERI; CLOSTRIDIUM SPOROGENES; CLOSTRIDIUM TYROBUTYRICUM; DARK FERMENTATION PIT MUD; FERMENTATION; FERMENTATION MONITORING; FERMENTATION TECHNIQUE; HALOTHERMOTHRIX; LACTOBACILLUS; LACTOBACILLUS BUCHNERI; LYSINIBACILLUS; MICROBIAL COMMUNITY; MICROBIAL DIVERSITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PEDIOCOCCUS ACIDILACTICI; POPULATION DYNAMICS; PRIORITY JOURNAL; ROSEBURIA; SPORANAEROBACTER; TISSIERELLA; BACTERIAL GENE; CLASSIFICATION; DENATURING GRADIENT GEL ELECTROPHORESIS; GENETICS; PHYLOGENY; POLYMERASE CHAIN REACTION; PROCEDURES; SPECIES DIFFERENCE; VALIDATION STUDY;

BASE SEQUENCE; CLOSTRIDIUM; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA PRIMERS; FERMENTATION; GENES, BACTERIAL; PHYLOGENY; POLYMERASE CHAIN REACTION; SPECIES SPECIFICITY;

EID: 84899500447     PISSN: 09608524     EISSN: 18732976     Source Type: Journal    
DOI: 10.1016/j.biortech.2014.04.008     Document Type: Article

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