Improvement of phylum- and class-specific primers for real-time PCR quantification of bacterial taxa

Journal of Microbiological Methods

Volumn 86, Issue 3, 2011, Pages 351-356

  Bacchetti De Gregoris, Tristano ^a   Aldred, Nick ^a   Clare, Anthony S ^a   Burgess, J Grant ^a  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

Author keywords

Bacteria; Broad taxonomic unit; QPCR; SYBR green

Indexed keywords

RNA 16S;

ACTINOBACTERIA; ALPHAPROTEOBACTERIA; ARTICLE; BACTERIAL GENE; BACTERIUM IDENTIFICATION; BACTEROIDETES; BIOFILM; DENATURING GRADIENT GEL ELECTROPHORESIS; FIRMICUTES; GAMMAPROTEOBACTERIA; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLUM; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA SEQUENCE; TAXONOMIC IDENTIFICATION; TAXONOMY;

ACTINOBACTERIA; AQUATIC ORGANISMS; BACTERIAL TYPING TECHNIQUES; BACTEROIDETES; BASE SEQUENCE; BIOFILMS; DNA PRIMERS; DNA, BACTERIAL; PHYLOGENY; PROTEOBACTERIA; REAL-TIME POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA;

ACTINOBACTERIA; BACTERIA (MICROORGANISMS); BACTEROIDETES; FIRMICUTES; GAMMAPROTEOBACTERIA; PROTEOBACTERIA;

EID: 79960648108     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2011.06.010     Document Type: Article

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