Assays for determining heparan sulfate and heparin O-sulfotransferase activity and specificity

Analytical and Bioanalytical Chemistry

Volumn 406, Issue 2, 2014, Pages 525-536

  Sterner, Eric ^a   Li, Lingyun ^b   Paul, Priscilla ^a   Beaudet, Julie M ^b   Liu, Jian ^d   Linhardt, Robert J ^a,b,c   Dordick, Jonathan S ^a,c  

^a RENSSELAER POLYTECHNIC INSTITUTE   (United States)

^b Rensselaer Polytechnic Institute   (United States)

^c RENSSELAER POLYTECHNIC INSTITUTE   (United States)

^d University of North Carolina at Chapel Hill   (United States)

Author keywords

Bioassays; Coupled assay; Enzymes; Heparan sulfate; Heparin; Mass spectrometry; Sulfotransferases

Indexed keywords

BIOASSAY; BIOCHEMISTRY; CLONING; ENZYMES; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; POLYSACCHARIDES; SULFUR COMPOUNDS;

BIO-MANUFACTURING; CHEMO-ENZYMATIC SYNTHESIS; COLORIMETRIC ASSAYS; HEPARAN SULFATES; HEPARIN; RADIOISOTOPIC METHODS; SCINTILLATION COUNTING; SULFOTRANSFERASES;

ASSAYS;

BACTERIA (MICROORGANISMS);

4 NITROPHENYL SULFATE; 4-NITROPHENYL SULFATE; HEPARAN SULFATE; HEPARIN; NITROBENZENE DERIVATIVE; SULFOTRANSFERASE; TYROSINE ESTER SULFOTRANSFERASE; TYROSINE-ESTER SULFOTRANSFERASE;

ARTICLE; BIOASSAY; CARBOHYDRATE ANALYSIS; CHEMISTRY; COLORIMETRY; ENZYME SPECIFICITY; KINETICS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR GENETICS;

BIOLOGICAL ASSAY; CARBOHYDRATE SEQUENCE; CHROMATOGRAPHY, LIQUID; COLORIMETRY; HEPARIN; HEPARITIN SULFATE; KINETICS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; NITROBENZENES; SUBSTRATE SPECIFICITY; SULFOTRANSFERASES;

EID: 84899477504     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-013-7470-4     Document Type: Article

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