Single Cell MicroRNA Analysis Using Microfluidic Flow Cytometry

PLoS ONE

Volumn 8, Issue 1, 2013, Pages

  Wu, Meiye ^a,b   Piccini, Matthew ^a   Koh, Chung Yan ^a   Lam, Kit S ^b,c   Singh, Anup K ^a  

^a SANDIA NATIONAL LABORATORIES   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CD69 ANTIGEN; MICRORNA 155; PHORBOL 13 ACETATE 12 MYRISTATE;

ARTICLE; CELL STIMULATION; FLOW CYTOMETRY; FLUORESCENCE IN SITU HYBRIDIZATION; GENE PRODUCT; HYDRODYNAMICS; LEUKEMIA CELL LINE; MICROFLUIDIC ANALYSIS; PROCESS DEVELOPMENT; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN LOCALIZATION; PROTEIN TARGETING; SINGLE CELL ANALYSIS; UPREGULATION;

ANTIGENS, CD; ANTIGENS, DIFFERENTIATION, T-LYMPHOCYTE; CELL DIFFERENTIATION; CELL PROLIFERATION; EQUIPMENT DESIGN; FLOW CYTOMETRY; HUMANS; IONOMYCIN; JURKAT CELLS; LECTINS, C-TYPE; LYMPHOCYTE ACTIVATION; MICROFLUIDIC ANALYTICAL TECHNIQUES; MICRORNAS; SINGLE-CELL ANALYSIS; T-LYMPHOCYTES; TETRADECANOYLPHORBOL ACETATE; UP-REGULATION;

EID: 84873851245     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0055044     Document Type: Article

References (31)

1. Alvarez-Garcia I, Miska EA, (2005) MicroRNA functions in animal development and human disease. Development 132: 4653-4662.
2. Baltimore D, Boldin MP, O'Connell RM, Rao DS, Taganov KD, (2008) MicroRNAs: new regulators of immune cell development and function. Nat Immunol 9: 839-845.
3. Lodish HF, Zhou B, Liu G, Chen CZ, (2008) Micromanagement of the immune system by microRNAs. Nat Rev Immunol 8: 120-130.
4. Kasinski AL, Slack FJ, (2011) Epigenetics and genetics. MicroRNAs en route to the clinic: progress in validating and targeting microRNAs for cancer therapy. Nat Rev Cancer 11: 849-864.
5. Alevizos I, Illei GG, (2010) MicroRNAs in Sjogren's syndrome as a prototypic autoimmune disease. Autoimmun Rev 9: 618-621.
6. Krutzfeldt J, Stoffel M, (2006) MicroRNAs: a new class of regulatory genes affecting metabolism. Cell Metab 4: 9-12.
7. Griffiths-Jones S, (2004) The microRNA Registry. Nucleic Acids Res 32: D109-111.
8. Valoczi A, Hornyik C, Varga N, Burgyan J, Kauppinen S, et al. (2004) Sensitive and specific detection of microRNAs by northern blot analysis using LNA-modified oligonucleotide probes. Nucleic Acids Res 32: e175.
9. Castoldi M, Schmidt S, Benes V, Noerholm M, Kulozik AE, et al. (2006) A sensitive array for microRNA expression profiling (miChip) based on locked nucleic acids (LNA). RNA 12: 913-920.
10. Thomson JM, Parker J, Perou CM, Hammond SM, (2004) A custom microarray platform for analysis of microRNA gene expression. Nat Methods 1: 47-53.
11. Duncan DD, Eshoo M, Esau C, Freier SM, Lollo BA, (2006) Absolute quantitation of microRNAs with a PCR-based assay. Anal Biochem 359: 268-270.
12. Raymond CK, Roberts BS, Garrett-Engele P, Lim LP, Johnson JM, (2005) Simple, quantitative primer-extension PCR assay for direct monitoring of microRNAs and short-interfering RNAs. RNA 11: 1737-1744.
13. Xu G, Fewell C, Taylor C, Deng N, Hedges D, et al. (2010) Transcriptome and targetome analysis in MIR155 expressing cells using RNA-seq. RNA 16: 1610-1622.
14. Nielsen BS, (2012) MicroRNA in situ hybridization. Methods Mol Biol 822: 67-84.
15. Tang F, Hajkova P, Barton SC, Lao K, Surani MA, (2006) MicroRNA expression profiling of single whole embryonic stem cells. Nucleic Acids Res 34: e9.
16. Tang F, Hajkova P, Barton SC, O'Carroll D, Lee C, et al. (2006) 220-plex microRNA expression profile of a single cell. Nat Protoc 1: 1154-1159.
17. Pena JT, Sohn-Lee C, Rouhanifard SH, Ludwig J, Hafner M, et al. (2009) miRNA in situ hybridization in formaldehyde and EDC-fixed tissues. Nat Methods 6: 139-141.
18. de Planell-Saguer M, Rodicio MC, Mourelatos Z, (2010) Rapid in situ codetection of noncoding RNAs and proteins in cells and formalin-fixed paraffin-embedded tissue sections without protease treatment. Nat Protoc 5: 1061-1073.
19. Wu M, Singh AK, (2012) Single-cell protein analysis. Curr Opin Biotechnol 23: 83-88.
20. Yilmaz S, Singh AK, (2012) Single cell genome sequencing. Curr Opin Biotechnol 23: 437-443.
21. Powell AA, Talasaz AH, Zhang H, Coram MA, Reddy A, et al. (2012) Single cell profiling of circulating tumor cells: transcriptional heterogeneity and diversity from breast cancer cell lines. PLoS One 7: e33788.
22. Liu P, Meagher RJ, Light YK, Yilmaz S, Chakraborty R, et al. (2011) Microfluidic fluorescence in situ hybridization and flow cytometry (muFlowFISH). Lab Chip 11: 2673-2679.
23. Wu M, Perroud TD, Srivastava N, Branda CS, Sale KL, et al. (2012) Microfluidically-unified cell culture, sample preparation, imaging and flow cytometry for measurement of cell signaling pathways with single cell resolution. Lab Chip 12: 2823-2831.
24. Gullberg M, Gustafsdottir SM, Schallmeiner E, Jarvius J, Bjarnegard M, et al. (2004) Cytokine detection by antibody-based proximity ligation. Proc Natl Acad Sci U S A 101: 8420-8424.
25. Leuchowius KJ, Weibrecht I, Soderberg O (2011) In situ proximity ligation assay for microscopy and flow cytometry. Curr Protoc Cytom Chapter 9: Unit 9 36.
26. Gustafsdottir SM, Schallmeiner E, Fredriksson S, Gullberg M, Soderberg O, et al. (2005) Proximity ligation assays for sensitive and specific protein analyses. Anal Biochem 345: 2-9.
27. Weiss A, Wiskocil RL, Stobo JD, (1984) The role of T3 surface molecules in the activation of human T cells: a two-stimulus requirement for IL 2 production reflects events occurring at a pre-translational level. J Immunol 133: 123-128.
28. Marzio R, Mauel J, Betz-Corradin S, (1999) CD69 and regulation of the immune function. Immunopharmacol Immunotoxicol 21: 565-582.
29. Lu LF, Thai TH, Calado DP, Chaudhry A, Kubo M, et al. (2009) Foxp3-dependent microRNA155 confers competitive fitness to regulatory T cells by targeting SOCS1 protein. Immunity 30: 80-91.
30. Martin P, Gomez M, Lamana A, Cruz-Adalia A, Ramirez-Huesca M, et al. (2010) CD69 association with Jak3/Stat5 proteins regulates Th17 cell differentiation. Mol Cell Biol 30: 4877-4889.
31. Thai TH, Calado DP, Casola S, Ansel KM, Xiao C, et al. (2007) Regulation of the germinal center response by microRNA-155. Science 316: 604-608.