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Volumn 7, Issue 1, 2012, Pages 118-127

Comprehensive qPCR profiling of gene expression in single neuronal cells

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL ASSAY; GENE EXPRESSION; GENE EXPRESSION PROFILING; HIGH THROUGHPUT SCREENING; INTERMETHOD COMPARISON; MICROFLUIDIC ANALYSIS; NERVE CELL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS;

EID: 84861115379     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2011.430     Document Type: Article
Times cited : (136)

References (35)
  • 1
    • 79958260092 scopus 로고    scopus 로고
    • The human brain in a dish: The promise of iPSC-derived neurons
    • Dolmetsch, R. & Geschwind, D.H. The human brain in a dish: the promise of iPSC-derived neurons. Cell 145, 831-834 (2011).
    • (2011) Cell , vol.145 , pp. 831-834
    • Dolmetsch, R.1    Geschwind, D.H.2
  • 2
    • 0023759044 scopus 로고
    • Amplifcation and analysis of DNA sequences in single human sperm and diploid cells
    • Li, H.H. et al. Amplifcation and analysis of DNA sequences in single human sperm and diploid cells. Nature 335, 414-417 (1988).
    • (1988) Nature , vol.335 , pp. 414-417
    • Li, H.H.1
  • 5
    • 77949458939 scopus 로고    scopus 로고
    • Identifcation of novel glial genes by single-cell transcriptional profling of Bergmann glial cells from mouse cerebellum
    • Koirala, S. & Corfas, G. Identifcation of novel glial genes by single-cell transcriptional profling of Bergmann glial cells from mouse cerebellum. PLoS One 5, e9198 (2011).
    • (2011) PLoS One , vol.5
    • Koirala, S.1    Corfas, G.2
  • 6
    • 0026787858 scopus 로고
    • AMPA receptor subunits expressed by single Purkinje cells
    • Lambolez, B. et al. AMPA receptor subunits expressed by single Purkinje cells. Neuron 9, 247-258 (1992).
    • (1992) Neuron , vol.9 , pp. 247-258
    • Lambolez, B.1
  • 7
    • 0026472487 scopus 로고
    • Dopamine receptor subtypes colocalize in rat striatonigral neurons
    • Surmeier, D.J. et al. Dopamine receptor subtypes colocalize in rat striatonigral neurons. Proc. Natl. Acad. Sci. USA 89, 10178-10182 (1992).
    • (1992) Proc. Natl. Acad. Sci. USA , vol.89 , pp. 10178-10182
    • Surmeier, D.J.1
  • 8
    • 21444454272 scopus 로고    scopus 로고
    • Single-cell transcriptional profiles and spatial patterning of the mammalian olfactory epithelium
    • DOI 10.1387/ijdb.041939it
    • Tietjen, I., Rihel, J. & Dulac, C.G. Single-cell transcriptional profles and spatial patterning of the mammalian olfactory epithelium. Int. J. Dev. Biol. 49, 201-207 (2005). (Pubitemid 40917340)
    • (2005) International Journal of Developmental Biology , vol.49 , Issue.SPEC. ISS. , pp. 201-207
    • Tietjen, I.1    Rihel, J.2    Dulac, C.G.3
  • 9
    • 0038702458 scopus 로고    scopus 로고
    • Single-cell transcriptional analysis of neuronal progenitors
    • DOI 10.1016/S0896-6273(03)00229-0
    • Tietjen, I. et al. Single-cell transcriptional analysis of neuronal progenitors. Neuron 38, 161-175 (2003). (Pubitemid 36555644)
    • (2003) Neuron , vol.38 , Issue.2 , pp. 161-175
    • Tietjen, I.1    Rihel, J.M.2    Cao, Y.3    Koentges, G.4    Zakhary, L.5    Dulac, C.6
  • 10
    • 0026793775 scopus 로고
    • Stimulus-induced coordinate changes in mRNA abundance in single postsynaptic hippocampal CA1 neurons
    • Mackler, S.A., Brooks, B.P. & Eberwine, J.H. Stimulus-induced coordinate changes in mRNA abundance in single postsynaptic hippocampal CA1 neurons. Neuron 9, 539-548 (1992).
    • (1992) Neuron , vol.9 , pp. 539-548
    • MacKler, S.A.1    Brooks, B.P.2    Eberwine, J.H.3
  • 11
    • 0027304782 scopus 로고
    • Diversity of glutamate receptor subunit mRNA expression within live hippocampal CA1 neurons
    • Mackler, S.A. & Eberwine, J.H. Diversity of glutamate receptor subunit mRNA expression within live hippocampal CA1 neurons. Mol. Pharmacol. 44, 308-315 (1993). (Pubitemid 23252235)
    • (1993) Molecular Pharmacology , vol.44 , Issue.2 , pp. 308-315
    • Mackler, S.A.1    Eberwine, J.H.2
  • 13
    • 0029040280 scopus 로고
    • PCR and patch-clamp analysis of single neurons
    • Sucher, N.J. & Deitcher, D.L. PCR and patch-clamp analysis of single neurons. Neuron 14, 1095-1100 (1995).
    • (1995) Neuron , vol.14 , pp. 1095-1100
    • Sucher, N.J.1    Deitcher, D.L.2
  • 14
    • 77952581678 scopus 로고    scopus 로고
    • MRNA-sequencing whole transcriptome analysis of a single cell on the SOLiD system
    • Lao, K.Q. et al. mRNA-sequencing whole transcriptome analysis of a single cell on the SOLiD system. J. Biomol. Tech. 20, 266-271 (2009).
    • (2009) J. Biomol. Tech. , vol.20 , pp. 266-271
    • Lao, K.Q.1
  • 15
    • 67349146589 scopus 로고    scopus 로고
    • MRNA-Seq whole-transcriptome analysis of a single cell
    • Tang, F. et al. mRNA-Seq whole-transcriptome analysis of a single cell. Nat. Methods 6, 377-382 (2009).
    • (2009) Nat. Methods , vol.6 , pp. 377-382
    • Tang, F.1
  • 16
    • 80052174976 scopus 로고    scopus 로고
    • High-throughput microfuidic single-cell RT-qPCR
    • White, A.K. et al. High-throughput microfuidic single-cell RT-qPCR. Proc. Natl. Acad. Sci. 108, 13999-14004 (2011).
    • (2011) Proc. Natl. Acad. Sci. , vol.108 , pp. 13999-14004
    • White, A.K.1
  • 17
    • 45849087630 scopus 로고    scopus 로고
    • High throughput gene expression measurement with real time PCR in a microfuidic dynamic array
    • Spurgeon, S.L., Jones, R.C. & Ramakrishnan, R. High throughput gene expression measurement with real time PCR in a microfuidic dynamic array. PLoS One 3, e1662 (2008).
    • (2008) PLoS One , vol.3
    • Spurgeon, S.L.1    Jones, R.C.2    Ramakrishnan, R.3
  • 18
    • 79960854285 scopus 로고    scopus 로고
    • Induction of human neuronal cells by defned transcription factors
    • Pang, Z.P. et al. Induction of human neuronal cells by defned transcription factors. Nature 476, 220-223 (2011).
    • (2011) Nature , vol.476 , pp. 220-223
    • Pang, Z.P.1
  • 19
    • 80051684096 scopus 로고    scopus 로고
    • MicroRNA-mediated conversion of human fbroblasts to neurons
    • Yoo, A.S. et al. MicroRNA-mediated conversion of human fbroblasts to neurons. Nature 476, 228-231 (2011).
    • (2011) Nature , vol.476 , pp. 228-231
    • Yoo, A.S.1
  • 20
    • 79952219919 scopus 로고    scopus 로고
    • Single cell transcriptional profling reveals heterogeneity of human induced pluripotent stem cells
    • Narsinh, K.H. et al. Single cell transcriptional profling reveals heterogeneity of human induced pluripotent stem cells. J. Clin. Invest. 121, 1217-1221 (2011).
    • (2011) J. Clin. Invest. , vol.121 , pp. 1217-1221
    • Narsinh, K.H.1
  • 21
    • 3242677098 scopus 로고    scopus 로고
    • Correlating function and gene expression of individual basal ganglia neurons
    • DOI 10.1016/j.tins.2004.06.007, PII S0166223604001912
    • Liss, B. & Roeper, J. Correlating function and gene expression of individual basal ganglia neurons. Trends Neurosci. 27, 475-481 (2004). (Pubitemid 38951040)
    • (2004) Trends in Neurosciences , vol.27 , Issue.8 , pp. 475-481
    • Liss, B.1    Roeper, J.2
  • 22
    • 77952382875 scopus 로고    scopus 로고
    • Cell type-specifc expression of acid-sensing ion channels in hippocampal interneurons
    • Weng, J.Y., Lin, Y.C. & Lien, C.C. Cell type-specifc expression of acid-sensing ion channels in hippocampal interneurons. J. Neurosci. 30, 6548-6558 (2010).
    • (2010) J. Neurosci. , vol.30 , pp. 6548-6558
    • Weng, J.Y.1    Lin, Y.C.2    Lien, C.C.3
  • 23
    • 36549064711 scopus 로고    scopus 로고
    • Neuroplasticity mediated by altered gene expression
    • DOI 10.1038/sj.npp.1301544, PII 1301544
    • McClung, C.A. & Nestler, E.J. Neuroplasticity mediated by altered gene expression. Neuropsychopharmacology 33, 3-17 (2008). (Pubitemid 350191310)
    • (2008) Neuropsychopharmacology , vol.33 , Issue.1 , pp. 3-17
    • McClung, C.A.1    Nestler, E.J.2
  • 24
    • 79958055503 scopus 로고    scopus 로고
    • Projection-specifc modulation of dopamine neuron synapses by aversive and rewarding stimuli
    • Lammel, S., Ion, D.I., Roeper, J. & Malenka, R.C. Projection-specifc modulation of dopamine neuron synapses by aversive and rewarding stimuli. Neuron 70, 855-862 (2011).
    • (2011) Neuron , vol.70 , pp. 855-862
    • Lammel, S.1    Ion, D.I.2    Roeper, J.3    Malenka, R.C.4
  • 25
    • 77957938882 scopus 로고    scopus 로고
    • Cell type-specifc loss of BDNF signaling mimics optogenetic control of cocaine reward
    • Lobo, M.K. et al. Cell type-specifc loss of BDNF signaling mimics optogenetic control of cocaine reward. Science 330, 385-390 (2010).
    • (2010) Science , vol.330 , pp. 385-390
    • Lobo, M.K.1
  • 26
    • 78650828094 scopus 로고    scopus 로고
    • The neuronal splicing factor Nova controls alternative splicing in N-type and P-type CaV2 calcium channels
    • Allen, S.E., Darnell, R.B. & Lipscombe, D. The neuronal splicing factor Nova controls alternative splicing in N-type and P-type CaV2 calcium channels. Channels (Austin) 4, 483-489 (2010).
    • (2010) Channels (Austin) , vol.4 , pp. 483-489
    • Allen, S.E.1    Darnell, R.B.2    Lipscombe, D.3
  • 27
    • 33646148029 scopus 로고    scopus 로고
    • Descrambling Dscam diversity
    • Bharadwaj, R. & Kolodkin, A.L. Descrambling Dscam diversity. Cell 125, 421-424 (2006).
    • (2006) Cell , vol.125 , pp. 421-424
    • Bharadwaj, R.1    Kolodkin, A.L.2
  • 28
    • 78149355198 scopus 로고    scopus 로고
    • Single-cell qPCR on dispersed primary pituitary cells-an optimized protocol
    • Hodne, K., Haug, T.M. & Weltzien, F.A. Single-cell qPCR on dispersed primary pituitary cells-an optimized protocol. BMC Mol. Biol. 11, 82 (2010).
    • (2010) BMC Mol. Biol. , vol.11 , pp. 82
    • Hodne, K.1    Haug, T.M.2    Weltzien, F.A.3
  • 29
    • 80355127997 scopus 로고    scopus 로고
    • Transcriptome analysis of single cells
    • published online, doi:10.3791/2634
    • Morris, J., Singh, J.M. & Eberwine, J.H. Transcriptome analysis of single cells. J. Vis. Exp. published online, doi:10.3791/2634 (2011).
    • (2011) J. Vis. Exp
    • Morris, J.1    Singh, J.M.2    Eberwine, J.H.3
  • 30
    • 34248662082 scopus 로고    scopus 로고
    • Split single-cell RT-PCR analysis of Purkinje cells
    • DOI 10.1038/nprot.2006.343, PII NPROT.2006.343
    • Esumi, S., Kaneko, R., Kawamura, Y. & Yagi, T. Split single-cell RT-PCR analysis of Purkinje cells. Nat. Protoc. 1, 2143-2151 (2006). (Pubitemid 46773337)
    • (2006) Nature Protocols , vol.1 , Issue.4 , pp. 2143-2151
    • Esumi, S.1    Kaneko, R.2    Kawamura, Y.3    Yagi, T.4
  • 31
    • 77953811012 scopus 로고    scopus 로고
    • An improved one-tube RT-PCR protocol for analyzing single-cell gene expression in individual mammalian cells
    • Li, Y. et al. An improved one-tube RT-PCR protocol for analyzing single-cell gene expression in individual mammalian cells. Anal. Bioanal. Chem. 397, 1853-1859 (2010).
    • (2010) Anal. Bioanal. Chem. , vol.397 , pp. 1853-1859
    • Li, Y.1
  • 32
    • 79955750055 scopus 로고    scopus 로고
    • Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum
    • Bendall, S.C. et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science 332, 687-696 (2011).
    • (2011) Science , vol.332 , pp. 687-696
    • Bendall, S.C.1
  • 33
    • 64149097786 scopus 로고    scopus 로고
    • The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments
    • Bustin, S.A. et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin. Chem. 55, 611-622 (2009).
    • (2009) Clin. Chem. , vol.55 , pp. 611-622
    • Bustin, S.A.1
  • 34
    • 0033990048 scopus 로고    scopus 로고
    • Primer3 on the WWW for general users and for biologist programmers
    • Rozen, S. & Skaletsky, H. Primer3 on the WWW for general users and for biologist programmers. Methods Mol. Biol. 132, 365-386 (2000).
    • (2000) Methods Mol. Biol. , vol.132 , pp. 365-386
    • Rozen, S.1    Skaletsky, H.2
  • 35
    • 79953047673 scopus 로고    scopus 로고
    • Real-time PCR (qPCR) primer design using free online software
    • Thornton, B. & Basu, C. Real-time PCR (qPCR) primer design using free online software. Biochem. Mol. Biol. Educ. 39, 145-154 (2011).
    • (2011) Biochem. Mol. Biol. Educ. , vol.39 , pp. 145-154
    • Thornton, B.1    Basu, C.2


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