Homocysteine induces cytotoxicity and proliferation inhibition in neural stem cells via DNA methylation in vitro

FEBS Journal

Volumn 281, Issue 8, 2014, Pages 2088-2096

  Lin, Ningning ^a   Qin, Shanchun ^a   Luo, Suhui ^a   Cui, Shanshan ^a   Huang, Guowei ^a   Zhang, Xumei ^a  

^a TIANJIN MEDICAL UNIVERSITY   (China)

Author keywords

DNA methylation; DNA methyltransferases; homocysteine; neural stem cells; proliferation

Indexed keywords

CYSTEINE; DNA METHYLTRANSFERASE 1; DNA METHYLTRANSFERASE 3A; DNA METHYLTRANSFERASE 3B; HOMOCYSTEINE; LACTATE DEHYDROGENASE; S ADENOSYLHOMOCYSTEINE; S ADENOSYLMETHIONINE; ZEBULARINE;

ANIMAL CELL; ANTIPROLIFERATIVE ACTIVITY; ARTICLE; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY; DNA METHYLATION; ENZYME ACTIVITY; HIPPOCAMPUS; NEURAL STEM CELL; NEUROTOXICITY; NEWBORN; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT;

DNA METHYLATION; DNA METHYLTRANSFERASES; HOMOCYSTEINE; NEURAL STEM CELLS; PROLIFERATION;

ANIMALS; CELL PROLIFERATION; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA METHYLATION; HOMOCYSTEINE; IMMUNOHISTOCHEMISTRY; NEURAL STEM CELLS; RATS; RATS, SPRAGUE-DAWLEY;

EID: 84899436522     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/febs.12764     Document Type: Article

References (26)

1. Herrmann W, &, Obeid R, (2011) Homocysteine: a biomarker in neurodegenerative diseases. Clin Chem Lab Med 49, 435-441.
2. Schalinske KL, &, Smazal AL, (2012) Homocysteine imbalance: a pathological metabolic marker. Adv Nutr 3, 755-762.
3. Vollset SE, Refsum H, Irgens LM, Emblem BM, Tverdal A, Gjessing HK, Monsen AL, &, Ueland PM, (2000) Plasma total homocysteine, pregnancy complications, and adverse pregnancy outcomes: the Hordaland homocysteine study. Am J Clin Nutr 71, 962-968.
4. den Heijer T, Vermeer SE, Clarke R, Oudkerk M, Koudstaal PJ, Hofman A, &, Breteler MM, (2003) Homocysteine and brain atrophy on MRI of non-demented elderly. Brain 126, 170-175.
5. Lee ES, Chen H, Soliman KF, &, Charlton CG, (2005) Effects of homocysteine on the dopaminergic system and behavior in rodents. Neurotoxicology 26, 361-371.
6. Wang J, Bai X, Chen Y, Zhao Y, &, Liu X, (2012) Homocysteine induces apoptosis of rat hippocampal neurons by inhibiting 14-3-3ε expression and activating calcineurin. PLoS One 7, e48247.
7. Ho PI, Ortiz D, Rogers E, &, Shea TB, (2002) Multiple aspects of homocysteine neurotoxicity: glutamate excitotoxicity, kinase hyperactivation and DNA damage. J Neurosci Res 70, 694-702.
8. Rabaneda LG, Carrasco M, López-Toledano MA, Murillo-Carretero M, Ruiz FA, Estrada C, &, Castro C, (2008) Homocysteine inhibits proliferation of neuronal precursors in the mouse adult brain by impairing the basic fibroblast growth factor signaling cascade and reducing extracellular regulated kinase 1/2-dependent cyclin E expression. FASEB J 22, 3823-3835.
9. Yan H, Zhang X, Luo S, Liu H, Wang X, Gao Y, Wilson JX, &, Huang G, (2013) Effects of homocysteine on ERK signaling and cell proliferation in fetal neural stem cells in vitro. Cell Biochem Biophys 66, 131-137.
10. Namihira M, Kohyama J, Abematsu M, &, Nakashima K, (2008) Epigenetic mechanisms regulating fate specification of neural stem cells. Philos Trans R Soc Lond B Biol Sci 363, 2099-2109.
11. Singh RP, Shiue K, Schomberg D, &, Zhou FC, (2009) Cellular epigenetic modifications of neural stem cell differentiation. Cell Transplant 18, 1197-1211.
12. Zhang XM, Huang GW, Tian ZH, Ren DL, &, X Wilson J, (2009) Folate deficiency induces neural stem cell apoptosis by increasing homocysteine in vitro. J Clin Biochem Nutr 45, 14-19.
13. Zhou L, Cheng X, Connolly BA, Dickman MJ, Hurd PJ, &, Hornby DP, (2002) Zebularine: a novel DNA methylation inhibitor that forms a covalent complex with DNA methyltransferases. J Mol Biol 321, 591-599.
14. Jaenisch R, &, Bird A, (2003) Epigenetic regulation of gene expression: how the genome integrates intrinsic and environmental signals. Nat Genet 33, 245-254.
15. Okano M, Bell DW, Haber DA, &, Li E, (1999) DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell 99, 247-257.
16. Hutnick LK, Golshani P, Namihira M, Xue Z, Matynia A, Yang XW, Silva AJ, Schweizer FE, &, Fan G, (2009) DNA hypomethylation restricted to the murine forebrain induces cortical degeneration and impairs postnatal neuronal maturation. Hum Mol Genet 18, 2875-2888.
17. Wu H, Coskun V, Tao J, Xie W, Ge W, Yoshikawa K, Li E, Zhang Y, &, Sun YE, (2010) Dnmt3a-dependent nonpromoter DNA methylation facilitates transcription of neurogenic genes. Science 329, 444-448.
18. Feng J, Chang H, Li E, &, Fan G, (2005) Dynamic expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the central nervous system. J Neurosci Res 79, 734-746.
19. Martins-Taylor K, Schroeder DI, LaSalle JM, Lalande M, &, Xu RH, (2012) Role of DNMT3B in the regulation of early neural and neural crest specifiers. Epigenetics 7, 71-82.
20. Chiang PK, (1998) Biological effects of inhibitors of S-adenosyl-homocysteine hydrolase. Pharmacol Ther 77, 115-134.
21. Obeid R, &, Herrmann W, (2006) Mechanisms of homocysteine neurotoxicity in neurodegenerative diseases with special reference to dementia. FEBS Lett 580, 2994-3005.
22. Wang Z, Liu DX, Wang FW, Zhang Q, Du ZX, Zhan JM, Yuan QH, Ling EA, &, Hao AJ, (2013) L-Cysteine promotes the proliferation and differentiation of neural stem cells via the CBS/H2S pathway. Neuroscience 237, 106-117.
23. Wang XF, &, Cynader MS, (2001) Pyruvate released by astrocytes protects neurons from copper-catalyzed cysteine neurotoxicity. J Neurosci 21, 3322-3331.
24. Luo S, Zhang X, Yu M, Yan H, Liu H, Wilson JX, &, Huang G, (2013) Folic acid acts through DNA methyltransferases to induce the differentiation of neural stem cells into neurons. Cell Biochem Biophys 66, 559-566.
25. Wang BX, Yin BL, He B, Chen C, Zhao M, Zhang WX, Xia ZK, Pan YZ, Tang JQ, Zhou XM, et al,. (2012) Overexpression of DNA damage-induced 45α gene contributes to esophageal squamous cell cancer by promoter hypomethylation. J Exp Clin Cancer Res 31, 11.
26. Cavallaro RA, Fuso A, D'Anselmi F, Seminara L, &, Scarpa S, (1994) The effect of S-adenosylmethionine on CNS gene expression studied by cDNA microarray analysis. Biochem Biophys Res Commun 205, 1748-1754.