Cx46 hemichannels contribute to the sodium leak conductance in lens fiber cells

American Journal of Physiology - Cell Physiology

Volumn 306, Issue 5, 2014, Pages

  Ebihara, Lisa ^a   Korzyukov, Yegor ^a   Kothari, Sorabh ^a   Tong, Jun Jie ^a  

^a ROSALIND FRANKLIN UNIVERSITY OF MEDICINE AND SCIENCE   (United States)

Author keywords

Connexin; Cx46; Hemichannel; Lens

Indexed keywords

CALCIUM; CONNEXIN 46; CONNEXIN 50; DIVALENT CATION; GAP JUNCTION PROTEIN; LANTHANUM; POTASSIUM CHANNEL; SODIUM; UNCLASSIFIED DRUG; EYE PROTEIN; HEMICHANNEL;

ANIMAL CELL; ARTICLE; IMMUNOLOCALIZATION; LENS FIBER; MEMBRANE CONDUCTANCE; MEMBRANE CURRENT; MEMBRANE POTENTIAL; MEMBRANE STEADY POTENTIAL; MOUSE; NONHUMAN; PH; POTASSIUM CONDUCTANCE; POTASSIUM CURRENT; PRIORITY JOURNAL; SODIUM CONDUCTANCE; STEADY STATE; TRANSGENIC MOUSE; WHOLE CELL PATCH CLAMP; ANIMAL; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; CHANNEL GATING; CX46; CYTOLOGY; GENETICS; ION TRANSPORT; KNOCKOUT MOUSE; LENS; METABOLISM;

CONNEXIN; CX46; HEMICHANNEL; LENS;

ANIMALS; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; CONNEXINS; EYE PROTEINS; ION CHANNEL GATING; ION TRANSPORT; LENS, CRYSTALLINE; MEMBRANE POTENTIALS; MICE; MICE, KNOCKOUT; SODIUM;

EID: 84899422740     PISSN: 03636143     EISSN: 15221563     Source Type: Journal    
DOI: 10.1152/ajpcell.00353.2013     Document Type: Article

References (32)

1. Becker D, Blase C, Bereiter-Hahn J, Jendrach M. TRPV4 exhibits a functional role in cell-volume regulation. J Cell Sci 118: 2435-2440, 2005.
2. Beedle AM, Hamid J, Zamponi GW. Inhibition of transiently expressed low-and high-voltage-activated calcium channels by trivalent metal cations. J Membr Biol 187: 225-238, 2002.
3. Contreras JE, Sanchez HA, Eugenin EA, Speidel D, Thiels M, Willecke K, Bukauskas FF, Bennett MV, Saez JC. Metabolic inhibition induces opening of unapposed connexin 43 gap junction hemichannels and reduces gap junctional communication in cortical astrocytes in culture. Proc Natl Acad Sci USA 99: 495-500, 2002.
4. Dahm R, van MJ, Prescott AR, Quinlan RA. Gap junctions containing alpha8-connexin (MP70) in the adult mammalian lens epithelium suggests a re-evaluation of its role in the lens. Exp Eye Res 69: 45-56, 1999.
5. Ebihara L. New roles for connexons. News Physiol Sci 18: 100-103, 2003.
6. Ebihara L, Steiner E. Properties of a nonjunctional current expressed from a rat connexin46 cDNA in Xenopus oocytes. J Gen Physiol 102: 59-74, 1993.
7. Ebihara L, Tong JJ, Vertel B, White TW, Chen TL. Properties of connexin 46 hemichannels in dissociated lens fiber cells. Invest Ophthalmol Vis Sci 52: 882-889, 2011.
8. Eskandari S, Zampighi GA, Leung DW, Wright EM, Loo DD. Inhibition of gap junction hemichannels by chloride channel blockers. J Membr Biol 185: 93-102, 2002.
9. Gerido DA, DeRosa AM, Richard G, White TW. Aberrant hemichannel properties of Cx26 mutations causing skin disease and deafness. Am J Physiol Cell Physiol 293: C337-C345, 2007.
10. Gunning SJ, Chung KK, Donaldson PJ, Webb KF. Identification of a nonselective cation channel in isolated lens fiber cells that is activated by cell shrinkage. Am J Physiol Cell Physiol 303: C1252-C1259, 2012.
11. John SA, Kondo R, Wang SY, Goldhaber JI, Weiss JN. Connexin-43 hemichannels opened by metabolic inhibition. J Biol Chem 274: 236-240, 1999.
12. Lansman JB. Blockade of current through single calcium channels by trivalent lanthanide cations. Effect of ionic radius on the rates of ion entry and exit. J Gen Physiol 95: 679-696, 1990.
13. Lee JR, DeRosa AM, White TW. Connexin mutations causing skin disease and deafness increase hemichannel activity and cell death when expressed in Xenopus oocytes. J Invest Dermatol 129: 870-878, 2009.
14. Liu HT, Toychiev AH, Takahashi N, Sabirov RZ, Okada Y. Maxianion channel as a candidate pathway for osmosensitive ATP release from mouse astrocytes in primary culture. Cell Res 18: 558-565, 2008.
15. Mathias RT, Kistler J, Donaldson P. The lens circulation. J Membr Biol 216: 1-16, 2007.
16. Mathias RT, White TW, Gong X. Lens gap junctions in growth, differentiation, and homeostasis. Physiol Rev 90: 179-206, 2010.
17. Mese G, Sellitto C, Li L, Wang HZ, Valiunas V, Richard G, Brink PR, White TW. The Cx26-G45E mutation displays increased hemichannel activity in a mouse model of the lethal form of keratitis-ichthyosisdeafness syndrome. Mol Biol Cell 22: 4776-4786, 2011.
18. Mhaske PV, Levit NA, Li L, Wang HZ, Lee JR, Shuja Z, Brink PR, White TW. The human Cx26-D50A and Cx26-A88V mutations causing keratitis-ichthyosis-deafness syndrome display increased hemichannel activity. Am J Physiol Cell Physiol 304: C1150-C1158, 2013.
19. Minogue PJ, Tong JJ, Arora A, Russell-Eggitt I, Hunt DM, Moore AT, Ebihara L, Beyer EC, Berthoud VM. A mutant connexin50 with enhanced hemichannel function leads to cell death. Invest Ophthalmol Vis Sci 50: 5837-5845, 2009.
20. Musil LS, Beyer EC, Goodenough DA. Expression of the gap junction protein connexin43 in embryonic chick lens: molecular cloning, ultrastructural localization, and post-translational phosphorylation. J Membr Biol 116: 163-175, 1990.
21. Paul DL, Ebihara L, Takemoto LJ, Swenson KI, Goodenough DA. Connexin46, a novel lens gap junction protein, induces voltage-gated currents in nonjunctional plasma membrane of Xenopus oocytes. J Cell Biol 115: 1077-1089, 1991.
22. Pfahnl A, Dahl G. Gating of Cx46 gap junction hemichannels by calcium and voltage. Pflügers Arch 437: 345-353, 1999.
23. Ren Q, Riquelme MA, Xu J, Yan X, Nicholson BJ, Gu S, Jiang JX. Cataract-causing mutation of human connexin 46 impairs gap junction, e74732, 2013.
24. Rong P, Wang X, Niesman I, Wu Y, Benedetti LE, Dunia I, Levy E, Gong X. Disruption of Gja8 (alpha8 connexin) in mice leads to microphthalmia associated with retardation of lens growth and lens fiber maturation. Development 129: 167-174, 2002.
25. Saez JC, Schalper KA, Retamal MA, Orellana JA, Shoji KF, Bennett MV. Cell membrane permeabilization via connexin hemichannels in living and dying cells. Exp Cell Res 316: 2377-2389, 2010.
26. Shahidullah M, Mandal A, Delamere NA. TRPV4 in porcine lens epithelium regulates hemichannel-mediated ATP release and Na-KATPase activity. Am J Physiol Cell Physiol 302: C1751-C1761, 2012.
27. Srinivas M, Kronengold J, Bukauskas FF, Bargiello T, Verselis V. Correlative studies of gating in Cx46 and Cx50 hemichannels and gap junction channels. Biophys J 88: 1725-1739, 2005.
28. Stout CE, Costantin JL, Naus CCG, Charles AC. Intercellular calcium signaling in astrocytes via ATP release through connexin hemichannels. J Biol Chem 277: 10482-10488, 2002.
29. Tenbroek EM, Johnson R, Louis CF. Cell-to-cell communication in a differentiating ovine lens culture system. Invest Ophthalmol Vis Sci 35: 215-228, 1994.
30. White TW, Goodenough DA, Paul DL. Targeted ablation of connexin50 in mice results in microphthalmia and zonular pulverulent cataracts. J Cell Biol 143: 815-25, 1998.
31. Willecke K, Heynkes R, Dahl E, Stutenkemper H, Hennemann S, Jungbluth T, Suchyna T, Nicholson BJ. Mouse connexin37: cloning and functional expression of a gap junction gene highly expressed in lung. J Cell Biol 114: 1049-1057, 1991.
32. Xia Ch Cheng C, Huang Q, Cheung D, Li L, Dunia I, Benedetti LE, Horwitz J, Gong X. Absence of alpha3 (Cx46) and alpha8 (Cx50) connexins leads to cataracts by affecting lens inner fiber cells. Exp Eye Res 83: 688-696, 2006.