Use of Bru-Seq and BruChase-Seq for genome-wide assessment of the synthesis and stability of RNA

Methods

Volumn 67, Issue 1, 2014, Pages 45-54

  Paulsen, Michelle T ^a   Veloso, Artur ^a,b   Prasad, Jayendra ^a   Bedi, Karan ^a,b   Ljungman, Emily A ^a   Magnuson, Brian ^a   Wilson, Thomas E ^c   Ljungman, Mats ^a,b  

^a UNIVERSITY OF MICHIGAN COMPREHENSIVE CANCER CENTER   (United States)

^b UNIVERSITY OF MICHIGAN   (United States)

^c UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

RNA splicing; RNA stability; Transcription

Indexed keywords

BROXURIDINE; URIDINE; 5-BROMOURIDINE; CD44 PROTEIN, HUMAN; COMPLEMENTARY DNA; HERMES ANTIGEN; MESSENGER RNA; PROTEIN P53; RETINOBLASTOMA PROTEIN; STOP CODON; TP53 PROTEIN, HUMAN;

ARTICLE; CELL LABELING; GENE EXPRESSION; GENOME ANALYSIS; HUMAN; HUMAN CELL; METHODOLOGY; PRIORITY JOURNAL; RNA DEGRADATION; RNA ISOLATION; RNA SPLICING; RNA STABILITY; RNA SYNTHESIS; RNA TRANSCRIPTION; STEADY STATE; ANALOGS AND DERIVATIVES; ANIMAL; BIOSYNTHESIS; CHEMISTRY; FRAMESHIFT MUTATION; GENETICS; HELA CELL LINE; HIGH THROUGHPUT SEQUENCING; HUMAN GENOME; ISOLATION AND PURIFICATION; K562 CELL LINE; KINETICS; METABOLISM; MOLECULAR GENETICS; SEQUENCE ANALYSIS; STAINING; STOP CODON;

ANIMALS; ANTIGENS, CD44; CODON, NONSENSE; DNA, COMPLEMENTARY; FRAMESHIFT MUTATION; GENOME, HUMAN; HELA CELLS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; K562 CELLS; KINETICS; MOLECULAR SEQUENCE ANNOTATION; RETINOBLASTOMA PROTEIN; RNA SPLICING; RNA STABILITY; RNA, MESSENGER; SEQUENCE ANALYSIS, RNA; STAINING AND LABELING; TUMOR SUPPRESSOR PROTEIN P53; URIDINE;

EID: 84899059500     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2013.08.015     Document Type: Article

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