Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping

Cancer Research

Volumn 59, Issue 1, 1999, Pages 141-150

  Macville, Merryn ^a,c   Schröck, Evelin ^a   Padilla Nash, Hesed ^a   Keck, Catherine ^b   Ghadimi, B Michael ^a   Zimonjic, Drazen ^b   Popescu, Nicholas ^b   Ried, Thomas ^a  

^a NATIONAL HUMAN GENOME RESEARCH INSTITUTE   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

^c RADBOUD UNIVERSITY NIJMEGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CANCER CYTODIAGNOSIS; CARCINOGENESIS; CHROMOSOME ABERRATION; CHROMOSOME ANALYSIS; CHROMOSOME MAP; COMPARATIVE GENOMIC HYBRIDIZATION; FLUORESCENCE IN SITU HYBRIDIZATION; HELA CELL; HUMAN; HUMAN CELL; KARYOTYPE; PRIORITY JOURNAL; UTERINE CERVIX CANCER;

CHROMOSOME ABERRATIONS; CYTOGENETICS; HELA CELLS; HUMANS;

EID: 0344076342     PISSN: 00085472     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (37)

1. Gey, G. O., Coffman, W. D., and Kubicek, M. T. Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium. Cancer Res., 12: 264-265, 1952.
2. Chen, T. R. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet., 48: 19-24, 1988.
3. Popescu, N. C., DiPaolo, J. A., and Amsbaugh, S. C. Integration sites of human papillomavirus 18 DNA sequences on HeLa cell chromosomes. Cytogenet. Cell Genet., 14: 58-62, 1987.
4. Stanbridge, E. J., Flandermeyer, R. R., Daniels, D. W., and Nelson-Rees, W. A. Specific chromosome loss associated with the expression of tumorigenicity in human cell hybrids. Somatic Cell Genet., 7: 699-712, 1981.
5. Lichy, J. H., Modi, W. S., Seaunez, H. N., and Howell, P. M. Identification of a human chromosome 11 gene which is differentially regulated in tumorigenic and non-tumorigenic somatic cell hybrids of HeLa cells. Cell Growth Differ., 3: 541-548, 1992.
6. Popescu, N. C., and DiPaolo, I. A. Preferential sites for viral integration on mammalian genome. Cancer Genet. Cytogenet., 42: 157-171, 1989.
7. Mincheva, A., Gissman, L., and Zur Hausen, H. Chromosomal integration sites of human papillomavirus DNA in three cervical cell lines mapped by in situ hybridization. Med. Microbiol. Immunol., 176: 245-256, 1987.
8. Heneen, W. K. HeLa cells and their possible contamination of other cells: karyotype studies. Hereditas, 82: 217-248, 1976.
9. Kraemer, P. M., Deaven, L. L., Crissman, H. A., Steinkamp, J. A., and Petersen, D. F. On the nature of heteroploidy. Cold Spring Harb. Symp. Quant. Biol., 38: 133-144, 1973.
10. Nelson-Rees, W. A., Hunter, L., Darlington, G. J., and O'Brien, S. J. Characteristics of HeLa strains: permanent vs. variable features. Cytogenet. Cell Genet., 27: 216-231, 1980.
11. Francke, U., Hammond, D. S., and Schneider, J. A. The band patterns of twelve D98/AH-2 marker chromosomes and their use for identification of intraspecific cell hybrids. Chromosoma (Berl.), 41: 111-121, 1973.
12. Ruess, D., Ye, L., and Grond-Ginsbach, C. HeLa D98/AH-2 studied by chromosome painting and conventional cytogenetic techniques. Chromosoma (Berl.), 102: 473-477, 1993.
13. Speicher, M. R., Ballard, S. G., and Ward, D. C. Karyotyping human chromosomes by combinatorial multi-fluor FISH. Nat. Genet., 12: 368-375, 1996.
14. Schröck, E., du Manoir, S., Veldman, T., Schoell, B., Wienberg, J., Ferguson-Smith, M. A., Ning, Y., Ledbetter, D. H., Bar-Am, I., Soenksen, D., Garini, Y., and Ried, T. Multicolor spectral karyotyping of human chromosomes. Science (Washington DC), 273: 494-497, 1996.
15. Garini, Y., Macville, M., du Manoir, S., Buckwald, R. A., Lavi, M., Katzir, N., Wine, D., Bar-Am, I., Schröck, E., Cabib, D., and Ried, T. Spectral karyotyping. Bioimaging, 4: 65-72, 1996.
16. Kallioniemi, A., Kallioniemi, O., Sudar, D., Rutovitz, D., Gray, J. W., Waldman, F., and Pinkel, D. Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. Science (Washington DC), 258: 818-821, 1992.
17. Zimonjic, D. B., and Popescu, N. C. An improved procedure for chromosome preparations from solid tumors. Cancer Genet. Cytogenet., 72: 161, 1994.
18. Macville, M., Veldman, T., Padilla-Nash, H., Wangsa, D., O'Brien, P., Schröck, E., and Ried, T. Spectral karyotyping, a 24-colour FISH technique for the analysis of chromosomal rearrangements. Histochem. Cell Biol., 108: 299-305, 1997.
19. Telenius, H., Pelmear, A. H., Tunnacliffe, A., Carter, N. P., Behmel, A., Ferguson-Smith, M. A., Nordenskjold, M., Pfragner, R., and Ponder, B. A. J. Cytogenetic analysis by chromosome painting using DOP-PCR amplified flow-sorted chromosomes. Genes Chromosomes Cancer, 4: 267-270, 1992.
20. Malik, Z., Dishi, M., and Garini, Y. Fourier transform multipixel spectroscopy and spectral imaging of protoporphyrin in single melanoma cells. Photochem. Photobiol., 63: 608-614, 1996.
21. Mitelman, F. (ed.). ISCN: An International System for Human Cytogenetic Nomenclature. Basel: S. Karger, 1995.
22. Sambrook, J., Fritsch E. F., and Maniatis, T. (eds.). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 1989.
23. du Manoir, S., Speicher, M. R., Joos, S., Schröck, E., Popp, S., Döhner, H., Kovacs, G., Robert-Nicoud, M., Lichter, P., and Cremer, T. Detection of complete and partial chromosome gains and losses by comparative genomic in situ hybridization. Hum. Genet., 90: 590-610, 1993.
24. Dunham, I., Lengauer, C., Cremer, T., and Featherstone, T. Rapid generation of chromosome specific alphoid DNA probes using the polymerase chain reaction. Hum. Genet., 88: 457-462, 1992.
25. Ried, T., Petersen, I., Holtgreve-Grez, H., Speicher, M. R., Schröck, E., du Manoir, S., and Cremer, T. Mapping of multiple DNA gains and losses in primary small cell lung carcinomas by comparative genomic hybridization. Cancer Res., 54: 1801-1806, 1994.
26. Ohta, M., Inoue, H., Cotticelli, M. G., Katstury, K., Baffa, R., Palazzo, J., Siprashvili, Z., Mori, M., McCue, P., Druck, T., Croce, C., and Huebner, K. The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers. Cell, 84: 587-597, 1996.
27. Zimonjic, D. B., Druck, T., Ohta, M., Kastury, K., Croce, C., Popescu, N. C., and Huebner. K. Position of chromosome 3p14.2 fragile site (FRA3B) within the FHIT gene. Cancer Res., 57: 1166-1170, 1997.
28. Zimonjic, D. B., Simpson, S., Popescu, N. C., and DiPaolo, J. A. Molecular cytogenetics of human papilloma virus-negative cervical carcinoma cell lines. Cancer Genet. Cytogenet., 82: 1-8, 1995.
29. Heselmeyer, K., Macville, M., Schröck, E., Blegen, H., Hellström, A-C., Shah, K., Auer, G., and Ried, T. Advanced stage cervical carcinomas are defined by a recurrent pattern of chromosomal aberrations revealing high genetic instability and a consistent gain of chromosome 3q. Genes Chromosomes & Cancer, 19: 233-240, 1997.
30. Popescu, N. C. Chromosome fragility and instability in human cancer. Crit. Rev. Oncol., 5: 121-140, 1994.
31. De Braekeleer, M., Sreekantaiah, C., and Haas, O. Herpes simplex virus and human papillomavirus sites correlate with chromosomal breakpoints in human cervical cancer Cancer Genet. Cytogenet., 59: 135-137, 1992.
32. Saxon, P. J., Srivatsan, E. S., and Stanbridge, E. J. Introduction of human chromosome 11 via microcell transfer controls tumorigenic expression of HeLa cells. EMBO J., 5: 3461-3466, 1986.
33. Hampton, G. M., Penny, L. A., Baergen, R. N., Larson, A., Brewer, C., Liao, S., and Busby-Earle, R. M. C. Loss of heterozygosity in cervical carcinoma: subchromosomal localization of a putative tumor-suppressor gene to chromosome 11q22-24. Proc. Natl. Acad. Sci. USA, 91: 6953-6957, 1994.
34. Misra, B. S., and Srivatsan, E. S. Localization of HeLa cell tumor-suppressor gene to the long arm of chromosome 11. Am. J. Hum. Genet., 45: 565-577, 1989.
35. Jesudusan, R. A., Slovak, M. L., Sen, S., and Srivatsan, E. S. Rearrangement of chromosome band 11q13 in HeLa cells. Anticancer Res., 14: 1727-1734, 1994.
36. Popescu, N. C., and DiPaolo, J. A. Cytogenetics of cervical neoplasia. Cancer Genet. Cytogenet., 60: 214-215, 1992.
37. Atkin, N. B. Cytogenetics of carcinoma of the cervix uteri: a review. Cancer Genet. Cytogenet., 95: 33-39, 1997.