A rapid and efficient immunoenzymatic assay to detect receptor protein interactions: G protein-coupled receptors

International Journal of Molecular Sciences

Volumn 15, Issue 4, 2014, Pages 6252-6264

  Zappelli, Elisa ^a   Daniele, Simona ^a   Abbracchio, Maria P ^b   Martini, Claudia ^a   Trincavelli, Maria Letizia ^a  

^a UNIVERSITY OF PISA   (Italy)

^b UNIVERSITY OF MILAN   (Italy)

Author keywords

G protein coupled receptors; Immunoenzymatic assay; Protein protein interactions

Indexed keywords

G PROTEIN COUPLED RECEPTOR; BETA ARRESTIN; GPR17 PROTEIN, HUMAN; RETINA S ANTIGEN;

ARTICLE; CELL CULTURE; CELL LYSATE; CONTROLLED STUDY; DENSITOMETRY; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME SPECIFICITY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; NONHUMAN; PROTEIN INTERACTION; RAT; SIGNAL TRANSDUCTION; ANIMAL; IMMUNOLOGY; IMMUNOPRECIPITATION; METABOLISM; PROTEIN PROTEIN INTERACTION; SPRAGUE DAWLEY RAT;

ANIMALS; ARRESTINS; CELLS, CULTURED; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; IMMUNOPRECIPITATION; PROTEIN INTERACTION MAPS; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, G-PROTEIN-COUPLED; SIGNAL TRANSDUCTION;

EID: 84898651980     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms15046252     Document Type: Article

References (21)

1. Fredriksson, R.; Lagerstrom, M.C.; Lundin, L.G.; Schioth, H.B. The G protei coupled receptors in the human genom eform five main families. Phylogenetic analysis, paralgon groups, and fingerprints. Mol. Pharmacol. 2003, 63, 1256-1272.
2. Robas, N.; O'Reilly, M.; Katugampola, S.; Fidock, M. Maximizing serendiSitra:t egies for identifying ligands for orphan -protein coupled receptors. Curr. Opin. Pharmacol.2003, 3, 121-126.
3. Bockaert, J.; Roussignol, G.; Bécamel, C.; Gavarini, S.; Joubert, L.; Dumuis, A.; Fagni, L.; Marin, P. GPCR-interacting proteins (GIPs): Nature and functions. Biochem. Soc. Trans. 2004, 32, 851-855.
4. Ferguson, S.S. Evolving concepts in G protein-coupled receptor endocytosis: The role in receptor desensitization and signaling. Pharmacol. Rev. 2001, 53, 1-24.
5. Gurevich, E.V.; Tesmer, J.J.; Mushegian, A.; Gurevich, V.V. G proteincoupled receptor kinases: More than just kinases and not only for GPCRs. Pharmacol. Ther. 2012, 133, 40-69.
6. Premont, R.T.; Gainetdinov, R.R. Physiological roles of G proteicoupled receptor kinases and arrestins. Annu. Rev. Physiol. 2007, 69, 511-534.
7. Pfleger, K.D.; Eidne, K. Monitoring the formation of dynamic G protein coupled receptor/protein complex in living cells. Biochem. J. 2005, 385, 625-637.
8. Wei, H.; Tymiak, A.A.; Chen, G. Higresolution MS for structural characterization of protein therapeutics: Advances and future directions. Bioanalysis 2013, 10, 1299-1313.
9. Von Mering, C.; Krause, R.; Snel, B.; Cornell, M. Comparative assessment of la-scale data sets of protein-protein interactions. Nature 2002, 417, 399-403.
10. Aloy, P.; Russell, R.B. Ten thousand interactions for the molecular biologi Ntat. Biotechnol. 2004, 22, 1317-1321.
11. Lu, L.; Arakaki, A.K.; Lu, H.; Skolnick, J. Multimeric threading-based prediction of protein-protein interactions on a genomic scaAep plication to the Saccharomyces cerevisiae proteome. Genome Res. 2003, 13, 1146-1154.
12. Ciana, P.; Fumagalli, M.; Trincavelli, M.L.; Verderio, C.; Rosa, P.; Lecca, D.; Ferrario, S.; Parravicini, C.; Capra, V.; Gelosa, P. et al. The orphan receptor GPR17 identified as a new dual uracil nucleotides/cysteinyl-leukotrienes receptor. EMBO J. 2006, 25, 4615-4627.
13. Fumagalli, M.; Daniele, S.; Lecca, D.; Le R.; PParravicini, C.; Fields, R.D.; Rosa, P.; Antonucci, F.; Verderio, C.; Trincavelli, M.L. et al. Phenotypic changes, signaling pathway, and functional correlates of GP x pressing neural precursor cells during oligodendrocyte differentiation. J. Biol. Chem. 2011, 286, 10593-10604.
14. Benned-Jensen, T.; Rosenkilde, M.M. Distinct expression and lig-abninding profiles of two constitutively active GPR17 splice variants. Br. J. Pharmacol. 2010, 159, 1092-1105.
15. Buccioni, M.; Marcucci, G.; dal Ben, D.; Giacobbe, D.L.; Lambertucci, C.; Soverchia, L.T.; Thomas, A.; Volpini, R.; Cristalli, GI.nnovative functional cAMP assay for studying G protein-coupled receptors: Application to the pharmacological characterization of GPR17. Purinergic Signal. 2011, 7, 463-468.
16. Lecca, D.; Trincavelli, M.L.; Gelosa, P.; Sironi, L.; Ciana, P.; Fumagalli, M.; Villa, G.; Verderio, C.; Grumelli, C.; Guerrini, U. et al. The recently identified P2Y-like receptor GPR17 is a sensor of brain damage and a new target for brain repair. PLoS One 2008, 3, 3579-3593.
17. Daniele, S.; Trincavelli, M.L; Gabelloni, P.; Lecca, D.; Rosa, P.; Abbracchio M.P.; Martini, C. Agonist-induced desensitization/resensitization of human G pcouep ed receptor 17: A functional cros-talk between purinergic and cystei lelukotriene ligands. J. Pharmacol. Exp. Ther. 2011, 338, 559-567.
18. Fratangeli, A.; Parmigiani, E.; Fumagalli, M.; Lecca, D.; Benfante, R.; Passafaro, M.; Buffo, A.; Abbracchio, M.P.; Rosa, P. The regulated expression, intracellular trafficking, and membrane recycling of the P2Y-like receptor GPR17 in Oli-neu oligodendroglial cells. J. Biol. Chem. 2013, 288, 5241-5256.
19. Daniele, S.; Trincavelli, M.L.; Fumagalli, M.; Zappelli, E.; Lecca, D.; Bonfanti, E.; Campiglia, P.; Abbracchio, M.P.; Martini, C. Does GRKβ arrestin machinery work as a "switch o" for GPR17-mediated activation of intracellular signalling pathw. Cyesll Signal. 2014, doi:10.1016/j.cellsig.2014.02.016.
20. DeWire, S.M.; Ahn, S.; Lefkowitz, R.J.; Shenoy, S. K-arr sttins and cell signaling. Annu. Rev. Physiol. 2007, 69, 483-510.
21. Reiter, E.; Lefkowitz, R.J. GRKs and β-arrestins: Roles in receptor silencing, trafficking and signaling. Trends Endocrinol. Metabol. 2006, 17, 159-165.