Binding of NAD+and L-threonine induces stepwise structural and flexibility changes in cupriavidus necator L-threonine dehydrogenase

Journal of Biological Chemistry

Volumn 289, Issue 15, 2014, Pages 10445-10454

  Nakano, Shogo ^a,b   Okazaki, Seiji ^a,b   Tokiwa, Hiroaki ^b,c   Asano, Yasuhisa ^a,b  

^a TOYAMA PREFECTURAL UNIVERSITY   (Japan)

^b RIKKYO UNIVERSITY   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CHAINS; ENZYME KINETICS; MOLECULAR DYNAMICS;

ACTIVE SITE; CATALYTIC DOMAINS; COMPUTATIONAL RESULTS; FLEXIBLE CONFORMATION; KINETICS PARAMETER; MOLECULAR DYNAMICS SIMULATIONS; REACTION PROGRESS; STRUCTURAL COMPARISON;

AMINO ACIDS;

MEMBRANE PROTEIN; PROTEIN NAD; THREONINE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CATALYSIS; CONFORMATION; CONTROLLED STUDY; CRYSTAL STRUCTURE; CRYSTALLIZATION; CUPRIAVIDUS NECATOR; ENZYME ACTIVE SITE; ENZYME KINETICS; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN MODIFICATION; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS;

CRYSTAL STRUCTURE; DEHYDROGENASE; ENZYME KINETICS; MOLECULAR DYNAMICS; NAD;

ALCOHOL OXIDOREDUCTASES; BACTERIAL PROTEINS; CATALYSIS; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; CUPRIAVIDUS NECATOR; KINETICS; MOLECULAR DYNAMICS SIMULATION; MUTAGENESIS, SITE-DIRECTED; NAD; PLASMIDS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; SENSITIVITY AND SPECIFICITY; THREONINE;

EID: 84898650507     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M113.540773     Document Type: Article

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