Characterization of Sulfolobus solfataricus β-galactosidase mutant F441Y expressed in Pichia pastoris

Journal of the Science of Food and Agriculture

Volumn 94, Issue 7, 2014, Pages 1359-1365

  Sun, Xiaojun ^a   Duan, Xuguo ^a   Wu, Dan ^a   Chen, Jian ^a   Wu, Jing ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Characterization; Expression; Pichia pastoris; galactosidase

Indexed keywords

BACTERIA (MICROORGANISMS); PICHIA PASTORIS; SULFOLOBUS SOLFATARICUS;

ARCHAEAL PROTEIN; BETA GALACTOSIDASE; GALACTOSE; LACTOSE; METHANOL; OLIGOSACCHARIDE; RECOMBINANT PROTEIN; SOLVENT;

AMINO ACID SUBSTITUTION; BIOREACTOR; BIOSYNTHESIS; CHEMISTRY; DRUG EFFECTS; ENZYME INDUCTION; ENZYME STABILITY; ENZYMOLOGY; GENETICS; GLYCOSYLATION; HEAT; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MICROBIOLOGY; MOLECULAR WEIGHT; MUTATION; PH; PICHIA; SULFOLOBUS SOLFATARICUS;

AMINO ACID SUBSTITUTION; ARCHAEAL PROTEINS; BETA-GALACTOSIDASE; BIOREACTORS; ENZYME INDUCTION; ENZYME STABILITY; GALACTOSE; GLYCOSYLATION; HOT TEMPERATURE; HYDROGEN-ION CONCENTRATION; KINETICS; LACTOSE; METHANOL; MOLECULAR WEIGHT; MUTATION; OLIGOSACCHARIDES; PICHIA; RECOMBINANT PROTEINS; SOLVENTS; SULFOLOBUS SOLFATARICUS;

EID: 84897962797     PISSN: 00225142     EISSN: 10970010     Source Type: Journal    
DOI: 10.1002/jsfa.6419     Document Type: Article

References (31)

1. Panesar PS, Panesar R, Singh RS, Kennedy JF and Kumar H, Microbial production, immobilization and applications of β-d-galactosidase. J Chem Technol Biotechnol 81:530-543 (2006).
2. Rosenberg ZM-M, Current trends of β-galactosidase application in food technology. J Food Nutr Res 45:47-54 (2006).
3. Mahoney RR, Galactosyl-oligosaccharide formation during lactose hydrolysis: a review. Food Chem 63:147-154 (1998).
4. Davies G and Henrissat B, Structures and mechanisms of glycosyl hydrolases. Structure 3:853-859 (1995).
5. Wu Y, Yuan S, Chen S, Wu D, Chen J and Wu J, Enhancing the production of galacto-oligosaccharides by mutagenesis of Sulfolobus solfataricus β-galactosidase. Food Chem 138:1588-1595 (2013).
6. Torres DP, Gonçalves MdPF, Teixeira JA and Rodrigues LR, Galacto-oligosaccharides: production, properties, applications, and significance as prebiotics. Compr Rev Food Sci Food Saf 9:438-454 (2010).
7. Chen W, Chen H, Xia Y, Zhao J, Tian F and Zhang H, Production, purification, and characterization of a potential thermostable galactosidase for milk lactose hydrolysis from Bacillus stearothermophilus. J Dairy Sci 91:1751-1758 (2008).
8. Chen W, Chen H, Xia Y, Yang J, Zhao J, Tian F, et al., Immobilization of recombinant thermostable β-galactosidase from Bacillus stearothermophilus for lactose hydrolysis in milk. J Dairy Sci 92:491-498 (2009).
9. Bakken AP, Hill CG and Amundson CH, Hydrolysis of lactose in skim milk by immobilized β-galactosidase (Bacillus circulans). Biotechnol Bioeng 39:408-417 (1992).
10. Roberfroid M, Health benefits of non-digestible oligosaccharides. Adv Exp Med Biol 427:211-219 (1997).
11. Barreteau H, Delattre C and Michaud P, Production of oligosaccharides as promising new food additive generation. Food Technol Biotechnol 44:323-333 (2006).
12. Chen X, Cao Y, Ding Y, Lu W and Li D, Cloning, functional expression and characterization of Aspergillus sulphureus β-mannanase in Pichia pastoris. J Biotechnol 128:452-461 (2007).
13. Katrolia P, Yan Q, Jia H, Li Y, Jiang Z and Song C, Molecular cloning and high-level expression of a β-galactosidase gene from Paecilomyces aerugineus in Pichia pastoris. J Mol Catal B 69:112-119 (2011).
14. Balamurugan V, Reddy GR and Suryanarayana VVS, Pichia pastoris: a notable heterologous expression system for the production of foreign proteins - vaccines. Indian J Biotechnol 6:175-186 (2007).
15. Tschopp JF, Brust PF, Cregg JM, Stillman CA and Gingeras TR, Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris. Nucleic Acids Res 15:3859-3876 (1987).
16. Moracci M, La Volpe A, Pulitzer JF, Rossi M and Ciaramella M, Expression of the thermostable beta-galactosidase gene from the archaebacterium Sulfolobus solfataricus in Saccharomyces cerevisiae and characterization of a new inducible promoter for heterologous expression. J Bacteriol 174:873-882 (1992).
17. Yuan T, Yang P, Wang Y, Meng K, Luo H, Zhang W, et al., Heterologous expression of a gene encoding a thermostable β-galactosidase from Alicyclobacillus acidocaldarius. Biotechnol Lett 30:343-348 (2008).
18. Chen D-L, Tong X, Chen S-W, Chen S, Wu D, Fang S-G, et al., Heterologous expression and biochemical characterization of α-glucosidase from Aspergillus niger by Pichia pastroris. J Agric Food Chem 58:4819-4824 (2010).
19. Wu Y, Qian L, Zhang F, Chen S and Wu J, Preliminary study on optimization of β-galactosidase production by recombinant E. coli. J Ind Microbiol 42:47-52 (2012).
20. Haki G and Rakshit S, Developments in industrially important thermostable enzymes: a review. Bioresour Technol 89:17-34 (2003).
21. Bu J, Yun Z and Xing L, Application and immobilization of β-galactosidase. Farm Prod Process 2:4-7 (2006).
22. Santos A, Ladero M and García-Ochoa F, Kinetic modeling of lactose hydrolysis by a β-galactosidase from Kluyveromices fragilis. Enzyme Microb Technol 22:558-567 (1998).
23. De Bales SA and Castillo FJ, Production of lactase by Candida pseudotropicalis grown in whey. Appl Environ Microbiol 37:1201-1205 (1979).
24. Tanaka Y, Kagamiishi A, Kiuchi A and Horiuchi T, Purification and properties of β-galactosidase from Aspergillus oryzae. J Biochem 77:241-247 (1975).
25. Wang HT and Hsu JT, Usage of enzyme substrate to protect the activities of cellulase, protease and α-amylase in simulations of monogastric animal and avian sequential total tract digestion. Asian Australas J Anim Sci 19:1164-1173 (2006).
26. Nasseau M, Boublik Y, Meier W, Winterhalter M and Fournier D, Substrate-permeable encapsulation of enzymes maintains effective activity, stabilizes against denaturation, and protects against proteolytic degradation. Biotechnol Bioeng 75:615-618 (2001).
27. Ladero M, Santos A, García J, Carrascosa A, Pessela B and García-Ochoa F, Studies on the activity and the stability of β-galactosidases from Thermus sp. strain T2 and from Kluyveromyces fragilis. Enzyme Microb Technol 30:392-405 (2002).
28. Boon M, Janssen A and Van't Riet K, Effect of temperature and enzyme origin on the enzymatic synthesis of oligosaccharides. Enzyme Microb Technol 26:271-281 (2000).
29. Gosling A, Stevens GW, Barber AR, Kentish SE and Gras SL, Effect of the substrate concentration and water activity on the yield and rate of the transfer reaction of β-galactosidase from Bacillus circulans. J Agric Food Chem 59:3366-3372 (2011).
30. Choi JJ, Oh EJ, Lee YJ, Suh DS, Lee JH, Lee SW, et al., Enhanced expression of the gene for β-glycosidase of Thermus caldophilus GK24 and synthesis of galacto-oligosaccharides by the enzyme. Biotechnol Appl Biochem 38:131-136 (2003).
31. Hung M-N, Xia Z, Hu N-T and Lee BH, Molecular and biochemical analysis of two β-galactosidases from Bifidobacterium infantis HL96. Appl Environ Microbiol 67:4256-4263 (2001).